【摘要】：目的：觀察IL-17在脂多糖致大鼠ALI模型的表達(dá),并觀察早期應(yīng)用小劑量地塞米松(1mg/kg)對其影響。 方法：將成年健康雄性SD大鼠48只隨機(jī)分為正常對照組(C組),LPS致ALI組(ALI組),地塞米松組(D組)。腹腔注射脂多糖5mg/kg制作大鼠ALI模型,后立即腹腔注射地塞米松lmg/kg制作D組,C組腹腔注射等劑量的生理鹽水。于2h、6h兩點(diǎn)各組處死8只大鼠,提取左肺BALF和右肺組織。測定各組肺臟濕/干質(zhì)量比值(W/D),以及各組肺組織病理形態(tài)學(xué)變化;ELISA法測定BALF中IL-17、TNF-α水平 結(jié)果：1BALF中IL-17含量與C組比較,ALI組2h點(diǎn)IL-17含量無明顯差異,6h點(diǎn)IL-17含量顯著升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；2h點(diǎn)D組IL-17含量與C組及ALI組無差異,6h點(diǎn)IL-17含量較ALJ組減少,但仍高于C組,其與其他兩組比較均有統(tǒng)計(jì)學(xué)意義(P0.05)2BALF中TNF-α含量與C組比較,ALI組TNF-α含量明顯增加,并隨時(shí)間延長而增加,兩組差異有統(tǒng)計(jì)學(xué)意義(P0.05),D組TNF-α含量較ALI組減少,但仍高于C組,其與其他兩組比較均有統(tǒng)計(jì)學(xué)意義(P0.05)。3肺組織W/D C組兩時(shí)間點(diǎn)差異無統(tǒng)計(jì)學(xué)意義,ALI組明顯升高,各時(shí)點(diǎn)與C組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05),D組較ALI組明顯降低,但仍高于C組。4肺組織病理學(xué)變化：ALI組兩肺出現(xiàn)程度不一的腫脹充血,重者可見散在出血點(diǎn)；光學(xué)顯微鏡可見彌漫性肺泡間隔增厚,部分肺泡萎陷,肺間質(zhì)彌漫性出血,肺泡腔可見大量炎性細(xì)胞浸潤及滲液。D組肺組織腫脹充血、肺泡腔炎性細(xì)胞浸潤及滲液、肺泡間隔增厚及肺泡萎陷程度均較ALI組明顯減輕。 結(jié)論：IL-17參與了LPS致大鼠ALI過程,6h時(shí)顯著表達(dá)。早期應(yīng)用小劑量地塞米松能降低大鼠ALI模型BALF中IL-17的表達(dá),可能是地塞米松減輕ALI的另一機(jī)制。
[Abstract]:Aim: to observe the expression of IL-17 in rat ALI model induced by lipopolysaccharide, and to observe the effect of low dose dexamethasone (1mg/kg) on it. Methods: Forty-eight adult male SD rats were randomly divided into normal control group (C group), LPS induced ALI group (ALI group) and dexamethasone group (D group). Rat ALI model was established by intraperitoneal injection of lipopolysaccharide (5mg/kg), then dexamethasone lmg/kg was injected intraperitoneally to make group D and group C was intraperitoneally injected with saline of the same dose. At 2 h and 6 h, 8 rats were killed in each group. BALF of left lung and right lung tissue were extracted. The wet / dry weight ratio of lung (W / D) and the pathological changes of lung tissue were measured in each group. The results of IL-17,TNF- 偽 determination in BALF by ELISA: compared with C group, the content of IL-17 in 1BALF group was not significantly different from that in group C at 2h point, but IL-17 content in ALI group was significantly increased at 6h point (P0.05). The content of IL-17 in group D was not different from that in group C and group ALI at 2h, but the content of IL-17 in group D was lower than that in group ALJ at 6 h, but still higher than that in group C. there was significant difference between group D and other two groups (P0.05) the content of TNF- 偽 in 2BALF was higher than that in group C (P0.05). The content of TNF- 偽 in ALI group was significantly higher than that in ALI group, and increased with time. The difference between the two groups was statistically significant (P0.05). The content of TNF- 偽 in), D group was lower than that in ALI group, but still higher than that in C group. Compared with the other two groups, there was statistical significance (P0.05). 3 there was no significant difference in lung tissue at two time points in WR / D C group, ALI group was significantly higher than that in C group (P0.05). Group D was significantly lower than group ALI, but still higher than group C. 4 the pathological changes of lung in group D were higher than those in group C. the swelling and congestion of the two lungs in ALI group were different in degree, and the heavy ones could be seen at the bleeding point. Optical microscope showed diffuse alveolar septum thickening, partial alveolar collapse, diffuse hemorrhage of pulmonary interstitial, infiltration and exudation of a large number of inflammatory cells in alveolar cavity, swelling and congestion of lung tissue, infiltration and exudation of inflammatory cells in alveolar cavity in group D. The thickening of alveolar septum and the degree of alveolar collapse were significantly reduced compared with ALI group. Conclusion: IL-17 was involved in the process of ALI induced by LPS in rats, and was significantly expressed at 6 h. Early application of low dose dexamethasone can reduce the expression of IL-17 in BALF of rat ALI model, which may be another mechanism of reducing ALI by dexamethasone.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R563.9

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