【摘要】：目的探討短時(shí)間內(nèi)反復(fù)冷空氣吸入刺激對(duì)活體動(dòng)物肺內(nèi)瞬時(shí)受體電位錨蛋白1(TRPA1)相關(guān)炎性因子的影響。方法雄性Wistar大鼠20只,隨機(jī)分為對(duì)照組、冷空氣吸入組、暖空氣吸入組和激動(dòng)劑刺激組,每組5只。冷空氣吸入組在麻醉后經(jīng)口氣管插管,接氣體溫度可控吸入裝置,吸入(2±1)℃冷空氣;暖空氣吸入組予以相同處置下吸入(36±1)℃暖空氣,每組均處置3次,1 h/次,兩次間隔12 h;激動(dòng)劑刺激組為室溫(24±2)℃情況下霧化吸入60 mmol/L丙烯醛,分3次,1 h/次,兩次間隔12 h。在最后一次處置結(jié)束后活殺大鼠,提取氣管及左上肺組織的總RNA,采用PCR方法明確肺TRPA1的表達(dá)后,觀察反復(fù)短時(shí)間冷空氣吸入刺激對(duì)大鼠肺內(nèi)TRPA1通道相關(guān)炎癥細(xì)胞炎性因子白介素1β(IL-1β)、白介素5(IL-5)、中性粒細(xì)胞趨化因子(CXCL-1/KC)mRNA表達(dá)的影響。結(jié)果 TRPA1在大鼠肺組織中有表達(dá),短時(shí)程冷空氣刺激組大鼠肺組織的IL-1β、IL-5、CXCL-1/KC mRNA的表達(dá)量均較暖空氣吸入組及正常對(duì)照組升高,TRPA1通道陽性激動(dòng)劑丙烯醛吸入組也出現(xiàn)相似結(jié)果。結(jié)論在活體動(dòng)物層次上,當(dāng)肺部受到短時(shí)有害低溫(低于17℃)刺激后,通過TRPA1活化途徑使肺內(nèi)一些炎性因子表達(dá)增加。
[Abstract]:Objective to investigate the effects of repeated cold air inhalation on inflammatory cytokines associated with transient receptor potential anchor protein 1 (TRPA1) in vivo. Methods Twenty male Wistar rats were randomly divided into control group, cold air inhalation group, warm air inhalation group and agonist stimulation group with 5 rats in each group. After anesthesia, the cold air inhalation group was intubated by oral tube and was connected with the gas temperature controlled inhalation device, and the cold air was inhaled at (2 鹵1) 鈩，

本文編號(hào)：2310714