PGD2調(diào)控TGF-β1在慢性阻塞性肺疾病氣道重塑中的作用
發(fā)布時間：2018-10-20 18:45
【摘要】：目的探討前列腺素D2(PGD2)對L-929小鼠肺成纖維細胞生物學特性的影響,利用其受體特異性抑制劑Laropiprant調(diào)控TGF-β1/Smads信號通路。方法按Laropiprant濃度梯度將細胞分為0.3μmoL組、1.0μmoL組、3.0μmoL組、10.0μmoL組、30.0μmoL,每組分別加入TGF-β2(2.5ng/mL)培養(yǎng)24h后使用相應(yīng)濃度的Laropiprant刺激24h,并分別用PCR法和Western blot法檢測細胞TGF-β1、Smad3以及Smad4的表達。分別采用不同濃度的Laropiprant用于細胞不同時間(12、24、48、72h),通過MTT法檢測Laropiprant對細胞生長的抑制作用。結(jié)果隨著Laropiprant的濃度增加,細胞TGF-β1、Smad3以及Smad4的mRNA和蛋白的表達呈下降趨勢,與對照組比較差異有統(tǒng)計學意義(P0.05)。不同濃度的Laropiprant作用于細胞不同時間后,細胞抑制率隨濃度增高和作用時間增長呈下降趨勢。結(jié)論 L-929小鼠肺成纖維細胞PGD2-DP1的表達可能與TGF-β1/Smads的調(diào)節(jié)相關(guān)。Laropiprant作用于細胞后,細胞抑制率隨濃度增高和作用時間增長呈下降趨勢。
[Abstract]:Objective to investigate the effects of prostaglandin D 2 (PGD2) on the biological characteristics of lung fibroblasts in L-929 mice, and to regulate the TGF- 尾 1/Smads signaling pathway by Laropiprant, a receptor specific inhibitor of prostaglandin D 2 (PGD2). Methods the cells were divided into 0. 3 渭 moL group, 1. 0 渭 moL group, 3. 0 渭 moL group, 10. 0 渭 moL group and 30. 0 渭 moL, group according to the Laropiprant concentration gradient. The cells were incubated with TGF- 尾 2 (2.5ng/mL) for 24 h. The expression of TGF- 尾 1 Smad3 and Smad4 were detected by PCR and Western blot methods. The inhibitory effects of Laropiprant on cell growth were detected by MTT assay at different concentrations of Laropiprant for different time (12 ~ (24) O ~ (48) ~ (72) h). Results with the increase of Laropiprant concentration, the expression of mRNA and protein of TGF- 尾 1, Smad3 and Smad4 decreased significantly compared with the control group (P0.05). After different concentrations of Laropiprant were treated for different time, the inhibition rate of cells decreased with the increase of concentration and time of action. Conclusion the expression of PGD2-DP1 in lung fibroblasts of L-929 mice may be related to the regulation of TGF- 尾 1/Smads. After treated with Laropiprant, the inhibitory rate of cells decreased with the increase of concentration and time of action.
【作者單位】： 貴州省遵義市第一人民醫(yī)院呼吸內(nèi)科;
【基金】：遵義市2010年科學技術(shù)基金項目[遵市科合字(2010)27號]
【分類號】：R563.9
[Abstract]:Objective to investigate the effects of prostaglandin D 2 (PGD2) on the biological characteristics of lung fibroblasts in L-929 mice, and to regulate the TGF- 尾 1/Smads signaling pathway by Laropiprant, a receptor specific inhibitor of prostaglandin D 2 (PGD2). Methods the cells were divided into 0. 3 渭 moL group, 1. 0 渭 moL group, 3. 0 渭 moL group, 10. 0 渭 moL group and 30. 0 渭 moL, group according to the Laropiprant concentration gradient. The cells were incubated with TGF- 尾 2 (2.5ng/mL) for 24 h. The expression of TGF- 尾 1 Smad3 and Smad4 were detected by PCR and Western blot methods. The inhibitory effects of Laropiprant on cell growth were detected by MTT assay at different concentrations of Laropiprant for different time (12 ~ (24) O ~ (48) ~ (72) h). Results with the increase of Laropiprant concentration, the expression of mRNA and protein of TGF- 尾 1, Smad3 and Smad4 decreased significantly compared with the control group (P0.05). After different concentrations of Laropiprant were treated for different time, the inhibition rate of cells decreased with the increase of concentration and time of action. Conclusion the expression of PGD2-DP1 in lung fibroblasts of L-929 mice may be related to the regulation of TGF- 尾 1/Smads. After treated with Laropiprant, the inhibitory rate of cells decreased with the increase of concentration and time of action.
【作者單位】： 貴州省遵義市第一人民醫(yī)院呼吸內(nèi)科;
【基金】：遵義市2010年科學技術(shù)基金項目[遵市科合字(2010)27號]
【分類號】：R563.9
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