【摘要】：肺組織內(nèi)出血時(shí)從血管中溢出的紅細(xì)胞被巨噬細(xì)胞吞噬，并在其溶酶體內(nèi)降解，，形成光鏡下可見的棕黃色顆粒，即為含鐵血黃素。這種巨噬細(xì)胞稱為含鐵血黃素細(xì)胞(hemosiderin-ladenmacrophage,HLM)。通常用普魯士藍(lán)染色涂片檢測。為了鑒定支氣管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）內(nèi)HLM對(duì)于肺部常見疾病診斷的指導(dǎo)意義，我們對(duì)2012年4月到10月期間在上海長海醫(yī)院進(jìn)行支氣管肺泡灌洗術(shù)檢查的102例住院患者進(jìn)行了回顧性的調(diào)查研究。將這些患者根據(jù)疾病診斷及各項(xiàng)臨床及生物學(xué)指標(biāo)進(jìn)行分組，同時(shí)將2012年4月到10月進(jìn)行BALF檢查的12例體檢正常者作為對(duì)照組。對(duì)送檢的BALF進(jìn)行涂片、普魯士藍(lán)法染色后，由兩名經(jīng)過專業(yè)培訓(xùn)的人員進(jìn)行HLM指數(shù)的計(jì)數(shù)。整理實(shí)驗(yàn)組與對(duì)照組數(shù)據(jù)，最后用SPSS軟件進(jìn)行HLM相關(guān)的統(tǒng)計(jì)分析。主要結(jié)果：（1）BALF中HLM與2項(xiàng)指標(biāo)密切相關(guān)：腎功能不全（Cr177μmoI/L）；吸煙（10包年）。（2）咯血患者的HLM較未出現(xiàn)咯血癥狀的患者多。（3）HLM在肺部真菌感染（尤其是侵襲性肺曲霉菌病）比肺部細(xì)菌、結(jié)核或病毒感染更易出現(xiàn)。（4）肺膿腫、支氣管擴(kuò)張患者BALF的HLM指數(shù)較高�？偟膩碚f，BALF中HLM對(duì)肺部疾病的診斷有一定指導(dǎo)作用。
[Abstract]:The red blood cells which overflow from the blood vessels during the hemorrhage of lung tissue were swallowed by macrophages and degraded in their lysosomes to form brown granules visible under light microscope, that is, hemosiderin. These macrophages are called hemosiderin cells (hemosiderin-ladenmacrophage,HLM). Prussian blue stains are usually used for detection. To identify the guiding significance of HLM in bronchoalveolar lavage fluid (bronchoalveolar lavage fluid,BALF) for the diagnosis of common lung diseases. A retrospective study was conducted on 102 inpatients who underwent bronchoalveolar lavage in Changhai Hospital from April to October 2012. These patients were divided into two groups according to disease diagnosis and clinical and biological indexes, and 12 normal persons who underwent BALF from April to October 2012 were used as control group. The BALF was smeared and stained by Prussian blue, and the HLM index was counted by two trained personnel. The data of the experimental group and the control group were collected. Finally, the HLM related statistical analysis was carried out with SPSS software. The main results were as follows: (1) HLM in BALF was closely related to two indexes: Cr177 渭 moI/L, HLM in patients with hemoptysis (10 packets of). (2) was higher than that in patients without hemoptysis. (3) HLM was more common in pulmonary fungal infection than in invasive pulmonary aspergillosis. Tuberculosis or viral infection is more likely to occur. (4) Pulmonary abscess, bronchiectasis patients with higher HLM index. In general, HLM in BALF has a guiding role in the diagnosis of pulmonary diseases.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R563

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相關(guān)期刊論文 前2條

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