發(fā)布時(shí)間：2018-09-04 11:09

【摘要】：目的探討醛糖還原酶在哮喘大鼠肺組織中表達(dá)的變化，以及與氣道炎癥、氣道重塑的關(guān)系。 方法健康雄性Wistar大鼠20只，體重210-250克，隨機(jī)分為兩組，即對(duì)照組與哮喘組，每組10只。采用卵清白蛋白致敏、激發(fā)動(dòng)物，復(fù)制大鼠的哮喘模型。對(duì)照組大鼠均以等量生理鹽水代替卵清白蛋白。收集肺泡灌洗液，檢測(cè)白細(xì)胞、嗜酸粒細(xì)胞計(jì)數(shù)。肺組織切片HE染色，觀察氣道形態(tài)并檢測(cè)形態(tài)學(xué)指標(biāo)。免疫組織化學(xué)法檢測(cè)醛糖還原酶（Aldose Reductase，AR）在兩組大鼠肺組織氣道壁的表達(dá)水平，采用圖像分析系統(tǒng)進(jìn)行圖象分析。 結(jié)果哮喘組大鼠肺泡灌洗液中白細(xì)胞（WBC）總數(shù)及嗜酸性粒細(xì)胞（Eos）計(jì)數(shù)高于對(duì)照組（P0.05）；哮喘組氣道壁面積WA/Pi、氣道平滑肌厚度（T1+T2)/Pi×100%較對(duì)照組明顯增加（P0.05）；哮喘組醛糖還原酶的表達(dá)水平顯著高于對(duì)照組(P0.05)；哮喘組大鼠肺組織中醛糖還原酶的表達(dá)水平與肺泡灌洗液中白細(xì)胞總數(shù)及嗜酸性粒細(xì)胞計(jì)數(shù)呈正相關(guān)(分別為r =0.887, r =0.387,P均0.05 )；而且醛糖還原酶的表達(dá)水平與WA/Pi、（T1+T2) /Pi×100%呈正相關(guān)(分別為r =0.889,r =0.983, P均0.05 )。 結(jié)論本實(shí)驗(yàn)采用卵清白蛋白（OVA）激發(fā)、致敏大鼠成功復(fù)制慢性哮喘模型，該模型的主要特征為氣道炎癥、氣道重塑。醛糖還原酶的表達(dá)與哮喘的氣道炎癥、氣道重塑呈正相關(guān)，，并可能參與氣道炎癥、氣道重塑的過(guò)程。
[Abstract]:Objective to investigate the expression of Aldose reductase in lung tissue of asthmatic rats and its relationship with airway inflammation and airway remodeling. Methods Twenty healthy male Wistar rats weighing 210-250g were randomly divided into two groups: control group and asthma group with 10 rats in each group. The asthmatic model of rats was induced by ovalbumin. In the control group, the ovalbumin was replaced by the same amount of normal saline. The alveolar lavage fluid was collected and leukocytes and eosinophils were measured. Lung tissue sections were stained with HE to observe the morphology of the airway and to detect the morphologic indexes. The expression of Aldose reductase (Aldose Reductase,AR) in the airway wall of two groups of rats was detected by immunohistochemical method. Image analysis system was used to analyze the expression of Aldose reductase (Aldose Reductase,AR). Results the total (WBC) count and eosinophil (Eos) count in alveolar lavage fluid of asthmatic rats were higher than those in the control group (P0.05), and the airway smooth muscle thickness (T1T2) / Pi 脳 100% of airway wall area (T1T2) / Pi 脳 100% in asthma group were significantly higher than those in control group (P0.05). The expression of Aldose reductase in asthma group was significantly higher than that in control group (P0.05). The expression of Aldose reductase in lung tissue of asthmatic rats was positively correlated with the total number of leukocytes and eosinophil count in alveolar lavage fluid (r = 0.887, r = 0.387, P 0.05, respectively). The expression level of Aldose reductase was positively correlated with WA/Pi, (T 1T 2) / Pi 脳 100% (r = 0.889r 0.983, P < 0.05). Conclusion the model of chronic asthma was successfully induced by ovalbumin (OVA) in sensitized rats. The main characteristics of the model were airway inflammation and airway remodeling. The expression of Aldose reductase was positively correlated with airway inflammation and airway remodeling, and may be involved in the process of airway inflammation and airway remodeling.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R562.25

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相關(guān)期刊論文 前2條

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本文編號(hào)：2221904