【摘要】：目的探討稀土氧化釹顆粒物(Nd2O3)致大鼠肺炎性損傷過(guò)程中,NF-κB炎性通路作用及其機(jī)制。方法選用SPF級(jí)健康成年雄性SD大鼠70只(體重200±10g),采用氣管非暴露插管灌注法進(jìn)行染塵,建立肺炎性損傷模型。采用稀土氧化釹粉塵懸液100mg/kg,0.8 ml和0.9%生理鹽水0.8ml一次性染塵,分別于染塵后第3、7、14、21、28d各處死7只實(shí)驗(yàn)組和7只對(duì)照組大鼠。肺組織HE染色,同時(shí)采用天狼猩紅染色,偏光顯微鏡下觀察Ⅰ、Ⅲ型膠原纖維。EMSA法測(cè)定大鼠新鮮肺組織中NF-κB的含量。Western blot法測(cè)定大鼠新鮮肺組織中p65、p-p65、PIKKβ蛋白的表達(dá)。取大鼠支氣管肺泡灌洗液(BALF),ELISA法分別測(cè)定實(shí)驗(yàn)組和對(duì)照組大鼠BALF中細(xì)胞因子的含量。結(jié)果(1)肺HE染色結(jié)果顯示肺炎性損傷模型造模成功。稀土氧化釹顆粒物致實(shí)驗(yàn)大鼠肺組織纖維化過(guò)程中可見(jiàn)Ⅰ、Ⅲ型膠原纖維的大量增生,染塵后早期主要以Ⅰ型膠原纖維增生為主。其Ⅰ、Ⅲ型膠原纖維明顯增加與對(duì)照組比較差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。(2)EMSA結(jié)果顯示實(shí)驗(yàn)組大鼠NF-κB活性增加;在14天達(dá)到最高值后又呈降低的變化趨勢(shì),但均高于對(duì)照組(P0.05);(3)實(shí)驗(yàn)組大鼠肺組織中p-p65、PIKKβ蛋白的表達(dá)均出現(xiàn)先增加后減少,在14天達(dá)到最高值后又呈下降的變化趨勢(shì),但均高于對(duì)照組(P0.05)。P65與對(duì)照組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);(4)實(shí)驗(yàn)組大鼠較對(duì)照組大鼠BALF中促炎性和抗炎性細(xì)胞因子均升高(P0.05),且在實(shí)驗(yàn)組中促炎性和抗炎性細(xì)胞因子均出現(xiàn)先增加后減少。在14天達(dá)到最高值后又呈下降的變化趨勢(shì),但均高于對(duì)照組(P0.05)。結(jié)論稀土氧化釹粉塵進(jìn)入大鼠肺組織后,啟動(dòng)了NF-κB炎性通路,導(dǎo)致肺組織中促炎性細(xì)胞因子和抗炎性細(xì)胞因子以及肺組織中蛋白含量均發(fā)生了明顯的變化,且Ⅰ、Ⅲ型膠原纖維大量增生,最終導(dǎo)致大鼠肺損傷。
[Abstract]:Objective to investigate the role and mechanism of NF- 魏 B inflammatory pathway in neodymium oxide particles (ND _ 2O _ 3) induced pneumonia injury in rats. Methods 70 healthy male Sprague-Dawley rats of SPF grade (body weight 200 鹵10 g) were used to establish pneumonic injury model. Neodymium oxide dust suspension (100mg 路kg ~ (-1) and 0.9% normal saline (0.8ml) were used to dye the dust at one time. Seven rats in the experimental group and seven in the control group were killed on the 28th day after exposure to neodymium oxide dust. Lung tissue was stained with HE and Sirius red staining. The content of NF- 魏 B in fresh lung tissue of rats was determined by polarizing microscope. The expression of p65- p-p65- PIKK 尾 in fresh lung tissue was detected by Western blot method. Bronchoalveolar lavage fluid (BALF) was used to detect cytokines in BALF of experimental group and control group. Results (1) Lung HE staining showed that the model of pneumonic injury was successful. During the process of pulmonary fibrosis induced by neodymium oxide particles, a large number of type 鈪，

本文編號(hào)：2119551