本文選題：機(jī)械通氣 + 呼吸機(jī)所致肺損傷��； 參考：《山西醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的通過觀察不同時間段機(jī)械通氣大鼠肺組織中小窩蛋白-1(caveolin-1, cav-1)的表達(dá)水平,探討cav-1在呼吸機(jī)所致肺損傷(VILI)發(fā)病中的作用。 方法雄性SD大鼠32只,被隨機(jī)分為對照組、大潮氣量機(jī)械通氣半小時組(H-VT0.5h)、大潮氣量機(jī)械通氣1小時組(H-VTlh)和大潮氣量機(jī)械通氣2小時組(H-VT2h)。于光鏡下觀察各組大鼠肺組織的病理學(xué)改變,測定其肺濕／干重比值(W/D)及BALF中總蛋白(TP)含量。各組大鼠肺組織cav-1的表達(dá)水平采用免疫組織化學(xué)法(SABC法)測定。 結(jié)果 1.肺組織cav-1表達(dá)結(jié)果顯示：H-VT0.5h組(0.456±0.055)和對照組(0.394±0.057)均明顯高于H-VT2h組(0.297±0.046)和H-VTlh組(0.318±0.051)(P值均0.001),H-VT0.5h組明顯高于對照組(P0.001),H-VTlh組明顯高于H-VT2h組(P0.009)。 2.肺組織W/D比值結(jié)果顯示：H-VT2h組(7.172±0.200)和H-VTlh組(5.598±0.227)均明顯高于對照組(4.139±0.263)和H-VT0.5h組(4.273±0.197)(P值均0.001),H-VT2h組明顯高于H-VTlh組(P0.001),H-VT0.5h組與對照組比較無統(tǒng)計學(xué)意義(P0.05)。 3. BALF總蛋白含量測定結(jié)果顯示：H-VT2h組(0.654±0.0444)和H-VTlh組(0.440±0.0434)均明顯高于對照組(0.233±0.0274)和H-VT0.5h組(0.243±0.0416)(P值均0.001),H-VT2h組明顯高于H-VTlh組(P0.001),H-VT0.5h組與對照組比較無統(tǒng)計學(xué)意義(P0.05)。 4.肺組織HE病理學(xué)結(jié)果顯示：隨著機(jī)械通氣時間延長(尤以1小時后明顯),大鼠肺組織損傷程度呈逐漸加重趨勢。 結(jié)論 1.肺組織cav-1在大潮氣量機(jī)械通氣不同時間段表達(dá)水平明顯不同,其含量表現(xiàn)為先增高后降低。提示肺組織cav-1高表達(dá)對VILI起一定保護(hù)作用。 2.大潮氣量機(jī)械通氣可通過抑制肺組織cav-1表達(dá),誘發(fā)和加重肺組織損傷。
[Abstract]:Objective to investigate the role of Cav-1 in the pathogenesis of lung injury (VILI) induced by ventilator by observing the expression level of caveolin-1 (Cav-1) in the lung tissue of rats with mechanical ventilation at different time periods.
Methods 32 male SD rats were randomly divided into control group, high tidal volume mechanical ventilation group (H-VT0.5h), large tidal volume mechanical ventilation (H-VTlh) and large tidal volume mechanical ventilation (H-VT2h). The pathological changes of lung tissue in each group were observed under light microscope, and the ratio of lung wet / dry weight (W/D) and total protein in BALF (W/D) and BALF were measured. Content of TP). The expression level of Cav-1 in lung tissue of each group was determined by immunohistochemical method (SABC).
Result
1. Cav-1 expression in lung tissue showed that group H-VT0.5h (0.456 + 0.055) and control group (0.394 + 0.057) were significantly higher than group H-VT2h (0.297 + 0.046) and H-VTlh (0.318 + 0.051) (P value 0.001), and H-VT0.5h group was significantly higher than that of control group (P0.001), and H-VTlh group was significantly higher than group H-VT2h (P0.009).
2. W/D ratio of lung tissue showed that group H-VT2h (7.172 + 0.200) and H-VTlh group (5.598 + 0.227) were significantly higher than that of control group (4.139 + 0.263) and H-VT0.5h group (4.273 + 0.197) (P value 0.001), H-VT2h group was significantly higher than that of H-VTlh group (P0.001), and there was no significant difference between the H-VT0.5h group and the control group (P0.05).
The total protein content of 3. BALF showed that group H-VT2h (0.654 + 0.0444) and H-VTlh group (0.440 + 0.0434) were significantly higher than that of control group (0.233 + 0.0274) and H-VT0.5h group (0.243 + 0.0416) (P value was 0.001), and H-VT2h group was significantly higher than that of H-VTlh group (P0.001), and there was no significant difference between the H-VT0.5h group and the control group (P0.05).
4. the pathological findings of lung tissue HE showed that with the prolongation of mechanical ventilation time (especially after 1 hours), the degree of lung tissue injury gradually increased.
conclusion
The expression level of Cav-1 in 1. lung tissues was significantly different in different time periods of large tidal volume mechanical ventilation, and its content was increased first and then decreased. It suggested that the high expression of Cav-1 in lung tissue has a certain protective effect on VILI.
2. high tidal volume ventilation can induce and aggravate lung injury by inhibiting the expression of Cav-1 in lung tissue.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R563

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本文編號：2112327