本文選題：紅霉素 + 氧化應(yīng)激��； 參考：《細胞與分子免疫學(xué)雜志》2014年04期

【摘要】：目的探討紅霉素(EM)對氧化應(yīng)激下人THP-1單核細胞糖皮質(zhì)激素抵抗的作用及機制。方法 EM預(yù)孵育THP-1細胞后予煙草煙霧提取物(CSE)刺激細胞,用質(zhì)粒脂質(zhì)體法將構(gòu)建好的組蛋白去乙酰化酶2沉默RNA(HDAC2-siRNA)轉(zhuǎn)染細胞,ELISA檢測細胞培養(yǎng)上清白細胞介素8(IL-8)的含量,實時熒光定量PCR(qRT-PCR)和Western blot法分析HDAC2表達情況。結(jié)果 EM組的IL-8抑制率明顯高于CSE組,而低于對照組(P0.05),EM組地塞米松半數(shù)抑制濃度(IC50-Dex)低于CSE組,而高于對照組(P0.05)。EM組HDAC2蛋白的表達高于CSE組,而低于對照組(P0.05)。除此之外,應(yīng)用HDAC2-siRNA轉(zhuǎn)染細胞后HDAC2 mRNA及HDAC2蛋白的表達均明顯低于對照組及空轉(zhuǎn)組,而加用EM后其表達明顯升高(P0.05)。結(jié)論 CSE刺激下的THP-1細胞對地塞米松的敏感性明顯降低,EM可通過上調(diào)HDAC2蛋白的表達減輕氧化應(yīng)激下THP-1細胞的糖皮質(zhì)激素抵抗。
[Abstract]:Objective to investigate the effect and mechanism of erythromycin (EM) on glucocorticoid resistance of human THP-1 monocytes under oxidative stress. Methods THP-1 cells were preincubated with EM and stimulated by tobacco smoke extract (CSE). The constructed histone deacetylase 2 silencing RNA (HDAC2-siRNA) transfected cells were transfected with HDAC2-siRNA to detect the interleukin-8 (IL-8) content in the supernatant by plasmid liposome method. The expression of HDAC2 was analyzed by real time quantitative PCR (qRT-PCR) and Western blot. Results the inhibition rate of IL-8 in EM group was significantly higher than that in CSE group, but lower than that in control group (P0.05). IC50-Dex in EM group was lower than that in CSE group, while the expression of HDAC2 protein in EM group was higher than that in CSE group (P0.05), but lower than that in control group (P0.05). In addition, the expression of HDAC2 mRNA and HDAC2 protein in HDAC2-siRNA transfected cells were significantly lower than those in control group and empty group, but the expression of HDAC2 mRNA and HDAC2 protein were significantly increased after treatment with EM (P0.05). Conclusion the sensitivity of THP-1 cells stimulated by CSE to dexamethasone is significantly reduced by up-regulating the expression of HDAC2 protein to alleviate the glucocorticoid resistance of THP-1 cells under oxidative stress.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院呼吸內(nèi)科;廣西醫(yī)科大學(xué)第一附屬醫(yī)院內(nèi)科ICU;
【基金】：國家自然科學(xué)基金(81260012,81160009)
【分類號】：R563.9

【參考文獻】

相關(guān)期刊論文 前2條

1 何志義;梁毅;梁秋麗;李梅華;鐘小寧;;香煙對小鼠C2C12成肌細胞分化的影響[J];中國病理生理雜志;2010年05期

2 孫雪皎;鐘小寧;何志義;覃向林;張揚;;慢性阻塞性肺疾病患者外周血單個核細胞糖皮質(zhì)激素抵抗的體外觀察[J];細胞與分子免疫學(xué)雜志;2013年05期

【共引文獻】

相關(guān)期刊論文 前6條

1 鄭小珍;劉積鋒;鐘蔚;吳文斌;檀月芳;黃玉仕;;小劑量羅紅霉素對吸煙哮喘患者氣道炎癥的影響[J];重慶醫(yī)學(xué);2013年28期

2 陳瑞;陳榮昌;陳新;;慢性阻塞性肺疾病患者深吸氣量與運動能力的關(guān)系[J];中國病理生理雜志;2011年05期

3 黃郁凱;李曉紅;潘宇;林秋雄;朱杰寧;江雪燕;葉力通;余細勇;;成年大鼠骨骼肌干細胞的制備與新型培養(yǎng)方法[J];中國病理生理雜志;2013年01期

4 Isa Jetté-C，

本文編號：2070466