本文選題：肺結(jié)核 + 結(jié)核分枝桿菌潛伏感染��； 參考：《中國病原生物學(xué)雜志》2017年04期

【摘要】：目的比較活動性肺結(jié)核患者、潛伏感染者與健康對照者外周血結(jié)核分枝桿菌特異性抗原刺激前后lncRNA1和lncRNA2的差異性表達(dá),探討其作為診斷結(jié)核分枝桿菌感染生物標(biāo)志物的可能性。方法收集活動性肺結(jié)核患者、潛伏感染者和健康對照者全血,分別在特異性抗原刺激前后提取RNA,利用實時熒光定量PCR方法檢測lncRNA1和lncRNA2的mRNA表達(dá)水平。應(yīng)用ELISA檢測血漿中IFN-γ和IL-2含量。結(jié)果結(jié)核分枝桿菌特異性抗原刺激前,結(jié)核病組、潛伏感染組全血lncRNA1相對表達(dá)量較健康對照組分別下降32%和64%;lncRNA2結(jié)核病組相對表達(dá)量是健康對照組的6.10倍;抗原刺激后,結(jié)核病組、潛伏感染組lncRNA1相對表達(dá)量較健康對照組分別下降33%和77%,lncRNA2潛伏感染組相對表達(dá)量較健康對照組下降84%。與刺激前相比,抗原刺激后健康對照組、結(jié)核病組lncRNA1相對表達(dá)量分別提高1.80倍和2.80倍,lncRNA2健康對照組、潛伏感染組分別提高3.50倍和1.86倍(P0.05)。健康組與潛伏感染組、結(jié)核病組與潛伏感染組、健康組與結(jié)核病組lncRNA1ROC曲線下面積(AUC)分別為0.700、0.752和0.400,lncRNA2的AUC分別為0.702、0.465和0.762。在結(jié)核特異性蛋白刺激前,結(jié)核病組IFN-γ、IL-2含量均高于健康組(P0.05),刺激后,結(jié)核病組及潛伏感染組與健康組相比較、潛伏感染組與結(jié)核病組相比較IFN-γ均增高(P0.05)。與刺激前相比,刺激后3組IFN-γ、IL-2誘導(dǎo)表達(dá)量均顯著增高(P0.05)。結(jié)論活動性結(jié)核病、潛伏感染和健康對照者全血結(jié)核特異性抗原刺激前后lncRNA1和lncRNA2均出現(xiàn)差異性表達(dá),結(jié)核病及潛伏感染者血漿IFN-γ含量增高。lncRNA1和lncRNA2可能是診斷肺結(jié)核感染的潛在生物標(biāo)志物。
[Abstract]:Objective to compare the differential expression of lncRNA1 and lncRNA2 between the latent infection and the healthy controls before and after the specific antigen stimulation of Mycobacterium tuberculosis in the peripheral blood, and to explore the possibility of it as a biomarker for the diagnosis of Mycobacterium tuberculosis. Methods the methods of collecting active tuberculosis, latent infection and health were collected. In the whole blood, RNA was extracted before and after the specific antigen stimulation, and the mRNA expression level of lncRNA1 and lncRNA2 was detected by real-time fluorescence quantitative PCR. The content of IFN- gamma and IL-2 in plasma was detected by ELISA. The results showed that the relative expression of lncRNA1 in the whole blood of the tuberculosis group and the latent infection group was more healthy before the specific antigen stimulation of Mycobacterium tuberculosis. The relative expression of lncRNA2 in the lncRNA2 group was 6.10 times as high as that in the healthy control group. After the antigen stimulation, the relative expression of lncRNA1 in the tuberculosis group and the latent infection group was 33% and 77%, respectively, and the relative expression of the lncRNA2 latent infection group was lower than that of the healthy control group, 84%. was compared with the pre stimulation, and the antigen stimulation was healthy. In the control group, the relative expression of lncRNA1 in the tuberculosis group was increased by 1.80 times and 2.80 times respectively. The lncRNA2 healthy control group, the latent infection group increased 3.50 times and 1.86 times (P0.05) respectively. The health group and the latent infection group, the tuberculosis group and the latent infection group, the area under the lncRNA1ROC curve of the health group and the tuberculosis group (AUC) were 0.700,0.752 and 0.400, lncRN, respectively. The AUC of A2 was 0.702,0.465 and 0.762. before the tuberculosis specific protein stimulation, and the IFN- gamma and IL-2 content in the tuberculosis group were all higher than that of the healthy group (P0.05). After the stimulation, the tuberculosis group and the latent infection group compared with the healthy group, the latent infection group compared with the tuberculosis group, the IFN- gamma increased (P0.05). Compared with the stimulation, the 3 groups of IFN- gamma, IL-2 induction were compared with the stimulation before the stimulation. The expression of lncRNA1 and lncRNA2 in active tuberculosis, latent infection and healthy controls were all differentially expressed before and after tuberculosis specific antigen stimulation. The increase of IFN- gamma content in the plasma of tuberculosis and latent infection.LncRNA1 and lncRNA2 may be a potential biomarker for the diagnosis of tuberculosis infection.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院病原微生物學(xué)與免疫學(xué)教研室;北京大學(xué)醫(yī)學(xué)部基礎(chǔ)醫(yī)學(xué)院醫(yī)學(xué)信息學(xué)系;
【基金】：國家"十二五"計劃重大傳染病防治專項結(jié)核病專項(No.2013ZX10003003-002-002)
【分類號】：R521
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本文編號：2065905