本文選題：呼吸機(jī)相關(guān)肺炎 + 機(jī)械通氣��； 參考：《實(shí)用醫(yī)學(xué)雜志》2017年09期

【摘要】：目的:探討TNF-α刺激不同VT通氣小鼠對細(xì)胞因子表達(dá)影響。方法:36只SPF級雄性BALB/c小鼠,隨機(jī)分三組:A組:自主呼吸4 h,n=12;S組:VT=6 m L/kg,機(jī)械通氣4 h,n=12;L組:VT=20 m L/kg,機(jī)械通氣4 h,n=12。各組再分2亞組:對照組(C組,尾靜脈注射生理鹽水,n=6)、實(shí)驗(yàn)組(T組,以20μg/kg尾靜脈注射TNF-α,n=6)。觀察肺組織形態(tài)學(xué)及細(xì)胞超微結(jié)構(gòu)變化;檢測血清和BALF炎性因子的表達(dá)、NF-κBm RNA及蛋白的表達(dá)。結(jié)果:L組肺呈水腫、部分實(shí)變,炎性細(xì)胞積聚,巨噬細(xì)胞膜、核染色質(zhì)、板層小體、微絨毛等損傷性改變,且T組較C組明顯;與A組及S組比較,L組血清、BALF中TNF-α,IL-1β,IL-6,IL-10表達(dá)及NF-κB m RNA及蛋白表達(dá)均明顯升高,有統(tǒng)計(jì)學(xué)意義(P0.05)。T組與C組比較,TNF-α,IL-1β,IL-6,IL-10表達(dá)及NF-κb m RNA及蛋白表達(dá)均明顯升高,有統(tǒng)計(jì)學(xué)意義(P0.05),且NF-κB表達(dá)與TNF-α、IL-1β,IL-6呈正相關(guān)。結(jié)論:TNF-α是VAP發(fā)病的關(guān)鍵因子,可能為臨床預(yù)防VAP提供新方法。
[Abstract]:Objective: to investigate the effect of TNF- 偽 stimulation on cytokine expression in VT ventilated mice. Methods Thirty six SPF male BALB / c mice were randomly divided into three groups: group A: group 1: 6 ml / kg, group 1: 6 ml / kg; group 1: VT 20 mL / kg; group 1: 20 ml / kg; Each group was subdivided into 2 subgroups: control group (group C, intravenous injection of normal saline 6) and experimental group (group T, 20 渭 g/kg intravenous injection of TNF- 偽 ntidine 6). The expression of serum and BALF inflammatory factors and the expression of NF- 魏 Bm mRNA and protein were observed. Results there were edema, partial consolidation, inflammatory cell accumulation, macrophage cell membrane, nuclear chromatin, lamellar body, microvilli and other injurious changes in group W L, and the changes in group T were more obvious than those in group C. Compared with group A and group S, the expression of TNF- 偽, IL-1 尾, IL-6, IL-10, NF- 魏 B mRNA and protein in serum of group A and group S were significantly higher than those in group A (P0.05). The expression of IL-6IL-10 and the expression of NF- 魏 b mRNA and protein in group T were significantly higher than those in group C (P0.05). There was significant difference (P0.05), and the expression of NF- 魏 B was positively correlated with TNF- 偽 and IL-1 尾 -IL-6. Conclusion: TNF- 偽 is a key factor in the pathogenesis of VAP, which may provide a new method for clinical prevention of VAP.
【作者單位】： 廣西婦幼保健院麻醉科;
【基金】：廣西自然科學(xué)基金項(xiàng)目(編號:2014GXNSFAA118181) 廣西醫(yī)療衛(wèi)生適宜技術(shù)研究與開發(fā)項(xiàng)目(編號:S201540)
【分類號】：R563.1

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