本文選題：哮喘 + 組胺�。� 參考：《延邊大學》2013年碩士論文

【摘要】：目的： 本研究利用哮喘模型小鼠,觀察知母皂苷(Saponins from Anemarrhena Asphodeloides Bunge, SAAB)對Thl和Th2細胞因子抑制分泌的影響。SAAB具有明顯的抗炎、抗氧化等藥理作用。盡管已證明知母皂苷具有很強的抗炎效果,但是對過敏性哮喘的治療作用及其機制尚未見報道。本實驗將觀察知母皂苷對對肥大細胞脫顆粒的影響,探討知母皂苷抗哮喘作用及其可能的作用機制。 方法： 通過Anti-DNP IgE誘導大鼠被動皮膚過敏反應(yīng)(PCA)實驗,利用伊文思藍染料觀察血管通透性,利用Anti-DNP IgE誘導肥大細胞脫顆粒,體外獲取大鼠腹腔肥大細胞,并分成五組,正常組,脫顆粒組,SAAB低濃度組(SAAB25); SAAB中濃度組(SAAB50); SAAB高濃度組組(SAAB100),用比色法測定SAAB在體內(nèi)對肥大細胞影響,體外實驗觀察SAAB對Anti-DNP IgE誘導組胺釋放、細胞內(nèi)鈣攝入及炎性介質(zhì)TNF-的影響。 結(jié)果： SAAB25～100μg/L濃度對肥大細胞吸光度無明顯影響,表明SAAB對肥大細胞的存活幾乎無影響。Anti-DNP IgE能明顯誘導肥大細胞脫顆粒,給予25μg/LSAAB預處理也可見明顯的肥大細胞脫顆粒,但50和100μg/L預處理肥大細胞脫顆粒不明顯,能明顯的抑制肥大細胞脫顆粒。正常組肥大細胞釋放組胺較少。與正常組比較,肥大細胞釋放組胺明顯增多(P0.05),與脫顆粒組比較SAAB各治療組均能抑制肥大細胞的組胺釋放,其中高、中(100、50μg/L)劑量組與模型組比較,有統(tǒng)計學意義(P0.05)。正常組伊文思藍滲出較少。正常組伊文思藍滲出很少。與正常組比較,模型組伊文思藍滲出明顯增多(P0.05),與模型組比較SAAB各治療組均能減少染料的滲出,其中高、中(300、150mg/kg)劑量組與模型組比較,有統(tǒng)計學意義(P0.05)。正常組肥大細胞鈣攝入量較少。與正常組比較,脫顆粒組肥大細胞鈣攝入量明顯增多(P0.05),與脫顆粒組比較SAAB提取物各治療組均能抑制肥大細胞鈣攝入量,其中高、中(100、50μg/L)劑量組與模型組比較,有統(tǒng)計學意義(P0.05)。 結(jié)論： (1)SAAB明顯抑制肥大細胞脫顆粒和組胺的釋放。 (2)SAAB明細抑制肥大細胞介導的大鼠血漿漏出。 (3)SAAB通過影響鈣離子內(nèi)流抑制肥大細胞脫顆粒。
[Abstract]:Aim: to investigate the effects of saponin Saponins from Anemarrhena Asphodeloides Bunge.Saab on the inhibition of Thl and Th2 cytokines in asthmatic mice. Although it has been proved that Anemarrhenoside has a strong anti-inflammatory effect, the therapeutic effect and its mechanism of allergic asthma have not been reported. In this experiment, we observed the effect of Anemarrhenoside on mast cell degranulation, and discussed the antiasthmatic effect of Aemarrhenoside and its possible mechanism. Methods: Anti-DNP IgE induced passive skin allergic reaction (PCA) in rats. The vascular permeability was observed by Evans blue dye, mast cell degranulation was induced by Anti-DNP IgE, and rat peritoneal mast cells were obtained in vitro and divided into five groups. Normal group, degranulated group, SAAB low concentration group, SAAB medium concentration group, SAAB50, SAAB high concentration group, SAAB100, the effect of Saab on mast cells in vivo was determined by colorimetry, and the effect of Saab on anti-DNP IgE induced histamine release was observed in vitro. Effects of intracellular calcium intake and inflammatory mediator TNF-. Results: SAAB 25 渭 g / L concentration had no significant effect on mast cell absorbance, suggesting that Saab had little effect on mast cell survival. Anti-DNP IgE could induce mast cell degranulation. Pretreatment with 25 渭 g / L SAAB also showed obvious mast cell degranulation, but 50 渭 g / L and 100 渭 g / L pretreatment was not obvious, and could inhibit mast cell degranulation obviously. In normal group, mast cells released less histamine. Compared with the normal group, mast cell released histamine increased significantly (P 0.05). Compared with the degranulation group, Saab could inhibit the histamine release of mast cell. The histamine release of mast cell was inhibited by SAAB. The histamine release of mast cell was significantly higher in the treatment group than that in the model group (P 0.05), and the dose of 50 渭 g / L was higher than that in the model group. In normal group, Evans blue exudate less. In the normal group, Evans blue exudates little. Compared with the normal group, the exudation of Evans blue in the model group was significantly higher than that in the model group. Compared with the model group, Saab treatment group could reduce the dye exudation, especially in the high dose group (300 mg / kg) compared with the model group (P 0.05). The calcium intake of mast cells in normal group was less. Compared with the normal group, the calcium intake of mast cells in the degranulated group was significantly increased (P 0.05). Compared with the degranulated group, Saab extract could inhibit the calcium intake of mast cells in all the treatment groups. The calcium intake of mast cells in the treatment group was higher than that in the model group. Conclusion: Saab can inhibit mast cell degranulation and histamine release. It can inhibit mast cell induced plasma leakage in rats. It can inhibit mast cell degranulation through the influence of calcium influx.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R562.25;R285.5

【參考文獻】

相關(guān)期刊論文 前10條

1 潘瑞;潘家華;;T效應(yīng)細胞通路在哮喘發(fā)病機制中的研究進展及靶向治療前景[J];安徽醫(yī)藥;2011年04期

2 張玉英;盛剛;秦怡;陰智敏;田正良;惠朋利;賀鵬;何云義;;止喘貼對哮喘大鼠血清中IFN-γ、IL-4表達的影響[J];光明中醫(yī);2009年01期

3 湯葳;鄧偉吾;;支氣管哮喘的特異性免疫治療[J];世界臨床藥物;2010年01期

4 唐丹;梁萍;蘇培媛;;支氣管哮喘患兒Th1/Th2細胞免疫平衡變化研究[J];中國醫(yī)藥科學;2011年10期

5 鄭伯強;王桂蘭;楊賽;;粉塵螨滴劑舌下特異性免疫治療對兒童咳嗽變異性哮喘的有效性[J];中國當代兒科雜志;2012年08期

6 徐湛;羅琿;;BCG-CpG-DNA對哮喘小鼠Th1/Th2平衡調(diào)節(jié)影響的實驗研究[J];貴陽中醫(yī)學院學報;2012年05期

7 張雪;潘家華;;IL-17在哮喘發(fā)病機制中免疫作用的研究進展[J];安徽醫(yī)藥;2012年12期

8 陳志軍;錢惠江;;吸入糖皮質(zhì)激素對哮喘患者血清白介素-10和免疫球蛋白E的影響[J];河北醫(yī)藥;2012年03期

9 叢悅;柳曉蘭;余祖胤;康利平;馬百平;從玉文;;知母皂苷抑制血小板聚集的活性成分篩選及構(gòu)效關(guān)系分析[J];解放軍醫(yī)學雜志;2010年11期

10 鄧云,徐秋萍,劉振權(quán),馬百平,熊呈琦,趙陽;知母皂苷化合物對腦缺血再灌注大鼠的保護作用[J];北京中醫(yī)藥大學學報;2005年02期

，

本文編號：2015146