本文選題：肺氣腫 + 腫瘤壞死因子α��； 參考：《天津醫(yī)藥》2014年05期

【摘要】：目的建立間歇低氧(IH)合并肺氣腫重疊綜合征(OS)大鼠模型,探討OS大鼠系統(tǒng)及血管內(nèi)皮炎癥狀態(tài),并觀察外周血內(nèi)皮祖細(xì)胞(EPC)水平的變化。方法自制熏箱對(duì)大鼠進(jìn)行16周的熏煙暴露造成大鼠肺氣腫,從13周開(kāi)始,在熏煙暴露同時(shí),通過(guò)程控產(chǎn)生預(yù)制的間歇低氧/再氧合(IH/ROX)環(huán)境對(duì)大鼠進(jìn)行IH暴露4周。60只雄性Wistar大鼠隨機(jī)分為正常組(A組)、IH組(B組)、肺氣腫組(C組)和OS組(D組),暴露結(jié)束后分別以ELISA法測(cè)定血漿及右頸總動(dòng)脈內(nèi)皮細(xì)胞中腫瘤壞死因子(TNF)-α、白介素(IL)-6水平,Real-time PCR法檢測(cè)血管內(nèi)皮細(xì)胞中RhoA mRNA含量,病理標(biāo)本中測(cè)定頸動(dòng)脈內(nèi)中膜厚度(IMT)占全層厚度比值(C-IMT%),流式細(xì)胞儀測(cè)定循環(huán)血中EPC數(shù)量。結(jié)果 D組血漿和血管內(nèi)皮細(xì)胞中TNF-α、IL-6水平以及血管內(nèi)皮細(xì)胞中RhoA mRNA水平、C-IMT%均高于A、B、C組,而EPC數(shù)量均低于其他3組(均P0.05)。結(jié)論 OS大鼠較單純IH或單純肺氣腫大鼠系統(tǒng)及內(nèi)皮損傷更加嚴(yán)重,且內(nèi)皮修復(fù)能力更差,增加了心血管疾病風(fēng)險(xiǎn)。
[Abstract]:Objective to establish a rat model of intermittent hypoxic IH (IH) combined with emphysema overlap syndrome (OS) and to investigate the inflammatory status of systemic and vascular endothelial cells in OS rats and to observe the changes of EPCs in peripheral blood endothelial progenitor cells (EPC). Methods the rats were exposed to fumigation for 16 weeks in a self-made fumigation box, which caused emphysema in the rats, and at the same time, the emphysema was induced at the same time as the smoke exposure at the beginning of 13 weeks. By programmed production of prefabricated intermittent hypoxia / reoxygenation (IH / Rox) environment, rats were exposed to IH for 4 weeks. 60 male Wistar rats were randomly divided into two groups: normal group (group A), IH group (group B), emphysema group (group C) and OS group (group D). Tumor necrosis factor TNF- 偽 in plasma and endothelium of right carotid artery was detected by Elisa, and RHPA mRNA in vascular endothelial cells was detected by real-time PCR at IL-6 level. The ratio of IMT to whole layer thickness of carotid artery was measured in pathological specimens and the quantity of EPC in circulating blood was measured by flow cytometry. Results the levels of TNF- 偽 and IL-6 in plasma and vascular endothelial cells and the level of RhoA mRNA in vascular endothelial cells in group D were higher than those in group A, and the number of EPC in group D was lower than that in group A (P 0.05). Conclusion the injury of system and endothelium in OS rats is more serious than that in simple IH or emphysema rats, and the ability of endothelial repair is worse, which increases the risk of cardiovascular disease.
【作者單位】： 天津醫(yī)科大學(xué)總醫(yī)院呼吸科;天津醫(yī)科大學(xué)研究生院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(項(xiàng)目編號(hào):81270144,30800507,81170071)
【分類(lèi)號(hào)】：R563.3

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