本文選題：樹突細胞 + 哮喘　； 參考：《中國全科醫(yī)學》2014年24期

【摘要】：目的探討負載卵清蛋白(OVA)的樹突細胞回輸至小鼠體內對過敏性哮喘的影響。方法將30只雄性BALB/c小鼠采用隨機數字表法分為3組,每組10只。健康對照組不做任何處理;哮喘組給予磷酸鹽緩沖液(PBS),然后致敏和激發(fā);OVA-DC干預組尾靜脈注射負載OVA的樹突細胞1×106個,然后致敏和激發(fā)。將小鼠骨髓細胞用重組小鼠粒系-巨核系集落刺激因子(rmGM-CSF)和重組白介素4(rmIL-4)誘導分化為樹突細胞,并通過形態(tài)學和流式細胞術檢測細胞表面的CD11c予以鑒定。用OVA和脂多糖(LPS)刺激后,流式細胞術檢測細胞表面的主要組織相容性復合體Ⅱ類分子(MHCⅡ)和CD86,以此判斷樹突細胞的成熟狀態(tài);混合淋巴細胞增殖試驗(MLR)檢測不同狀態(tài)的樹突細胞刺激淋巴細胞增殖的能力。通過尾靜脈注射樹突細胞至小鼠體內,然后在特定時間進行致敏和激發(fā),觀察小鼠的生存狀態(tài)、測定脾臟質量;激發(fā)后24 h收集小鼠肺泡灌洗液檢測組胺水平;取肺組織行蘇木素-伊紅(HE)染色,鏡檢觀察肺部炎癥嚴重程度。結果小鼠骨髓細胞經rmGM-CSF和IL-4誘導分化為樹突細胞,樹突細胞在負載OVA后免疫表型未發(fā)生明顯的變化,仍處于未成熟狀態(tài),刺激淋巴細胞增殖的能力也很微弱。3組脾臟質量和肺泡灌洗液組胺水平比較,差異有統計學意義(P0.05),OVA-DC干預組較健康對照組和哮喘組升高,哮喘組較健康對照組升高(P0.05)。HE染色顯示OVA-DC干預組小鼠肺組織氣道管壁增厚,周圍有大量炎性細胞浸潤;哮喘組小鼠炎性反應較輕;健康對照組小鼠未發(fā)現炎性反應的特征。結論負載OVA后的樹突細胞通過尾靜脈回輸至小鼠體內可以促進過敏性哮喘的發(fā)生,樹突細胞在過敏性哮喘的發(fā)生機制方面發(fā)揮重要的作用。
[Abstract]:Objective to investigate the effect of dendritic cells loaded with ovalbumin (OVA) on allergic asthma in mice. Methods 30 male BALB / c mice were randomly divided into 3 groups with 10 mice in each group. The asthmatic group was treated with phosphate buffer (PBSN), then sensitized and stimulated by OVA-DC injection of OVA loaded dendritic cells (1 脳 106), then sensitized and stimulated. Mouse bone marrow cells were induced to differentiate into dendritic cells by recombinant granulocyte-megakaryocyte colony stimulating factor rmGM-CSF and recombinant interleukin-4rmIL-4. CD11c on the cell surface was detected by morphological and flow cytometry. After stimulation with OVA and lipopolysaccharide (LPS), the main histocompatibility complex 鈪，

本文編號：1988061