本文選題：抗菌肽 + 耐甲氧西林金黃色葡萄球菌　； 參考：《中國(guó)藥房》2017年23期

【摘要】：目的:了解抗菌肽對(duì)耐甲氧西林金黃色葡萄球菌(MRSA)的抗菌機(jī)制,以期為抗菌肽臨床治療MRSA肺炎提供參考。方法:查閱近年來國(guó)內(nèi)外相關(guān)文獻(xiàn),就各種抗菌肽對(duì)MRSA的抗菌機(jī)制的相關(guān)研究進(jìn)行歸納和總結(jié)。結(jié)果與結(jié)論:抗菌肽相對(duì)于抗菌藥物擁有較多優(yōu)勢(shì)——(1)抗菌肽是生物天然免疫系統(tǒng)的組成部分,容易獲得;抗菌肽氨基酸數(shù)較少、肽鏈較短,減小了合成抗菌肽的難度,為大量人工合成抗菌肽提供了可能性。(2)抗菌肽表面富含正電荷,YD1、Melittin和Bac8c均通過其表面的正電荷與MRSA表面的負(fù)電荷結(jié)合并黏附于細(xì)菌表面,進(jìn)一步破壞細(xì)胞膜從而殺滅細(xì)菌;LL-37能抑制MRSA生物膜的形成并破壞已經(jīng)形成的MRSA生物膜;h BD3-CBD通過在MRSA周圍聚集進(jìn)而發(fā)揮殺菌作用;J-AA、J-RR和J-AR利用其結(jié)構(gòu)特殊性,通過內(nèi)/外膜透化機(jī)制,破壞MRSA細(xì)胞膜,從而殺傷細(xì)菌。上述機(jī)制皆不涉及受體與配體之間的結(jié)合,避免了MRSA對(duì)抗菌肽產(chǎn)生耐藥性。(3)大部分抗菌肽在極低的MIC下即已對(duì)MRSA展示出了強(qiáng)大的殺菌作用�？咕牡氖褂靡泊嬖谝欢ǖ木窒扌浴�(1)抗菌肽的肽鏈較短,增加了提取難度,人工合成抗菌肽則提高了藥物成本。(2)抗菌肽的短肽鏈和簡(jiǎn)單結(jié)構(gòu),使其穩(wěn)定性較差。(3)抗菌肽是一種異種蛋白,可能誘發(fā)患者產(chǎn)生一系列的免疫反應(yīng)和毒性作用。
[Abstract]:Objective: to investigate the antibacterial mechanism of antimicrobial peptides against methicillin-resistant Staphylococcus aureus (MRSA) and to provide reference for clinical treatment of MRSA pneumonia with antimicrobial peptides. Methods: the research on antibacterial mechanism of various antimicrobial peptides against MRSA was summarized by referring to the related literatures at home and abroad in recent years. Results & conclusion: antimicrobial peptides have more advantages than antimicrobial agents. Antimicrobial peptides are an integral part of biological innate immune system and are easy to obtain, and antimicrobial peptides have fewer amino acids and shorter peptide chains, thus reducing the difficulty of synthesizing antimicrobial peptides. It provides a possibility for a large number of synthetic antimicrobial peptides.) the surface of antimicrobial peptides is rich in positive charge. Both YD1 and Bac8c bind to and adhere to the bacterial surface through the positive charge on the surface of MRSA and negative charge on the surface of MRSA. Further destroying cell membrane and killing bacteria LL-37 can inhibit the formation of MRSA biofilm and destroy the formed MRSA biofilm, BD3-CBD can play a bactericidal effect by aggregating around MRSA. Through the internal / outer membrane permeation mechanism, the MRSA cell membrane is destroyed, thereby killing bacteria. These mechanisms do not involve binding between receptors and ligands, and avoid the resistance of MRSA to antimicrobial peptides. (3) most antimicrobial peptides have shown a strong bactericidal effect on MRSA at very low MIC. The use of antimicrobial peptides also has some limitations: (1) the peptide chain of antimicrobial peptide is relatively short, which increases the difficulty of extraction, while the synthetic antibacterial peptide increases the cost of the drug, and the short peptide chain and simple structure of antimicrobial peptide. The antimicrobial peptide is a kind of heterologous protein, which may induce a series of immune reaction and toxicity in patients.
【作者單位】： 南方醫(yī)科大學(xué)珠江醫(yī)院呼吸內(nèi)科;南方醫(yī)科大學(xué)第二臨床醫(yī)學(xué)院;
【基金】：廣東省自然科學(xué)基金項(xiàng)目(No.2016A030313620) 南方醫(yī)科大學(xué)國(guó)家級(jí)、省級(jí)、校級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(No.201612121002)
【分類號(hào)】：R563.1
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本文編號(hào)：1964621