本文選題：肺結(jié)核 + 維生素D受體（VDR）基因　； 參考：《寧夏醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的：采用病例對(duì)照研究方法，探討維生素D受體（VDR）基因多態(tài)性與寧夏回族自治區(qū)人群肺結(jié)核病易感性之間的關(guān)系。 方法：病例組來自寧夏回族自治區(qū)，2010年經(jīng)各醫(yī)院專家按照WHO肺結(jié)核病診斷標(biāo)準(zhǔn)確診的肺結(jié)核患者，以與病例組性別相同、民族相同、年齡相當(dāng)、居住環(huán)境相匹配的確認(rèn)無肺結(jié)核病者為對(duì)照組。病例對(duì)照組采集2-5mL靜脈血、肺結(jié)核相關(guān)的環(huán)境因素進(jìn)行問卷調(diào)查。采用聚合酶鏈?zhǔn)椒磻?yīng)-限制性片段長度多態(tài)性分析（PCR-RFLP）檢測(cè)VDR-rs731236、rs2228570位點(diǎn)基因多態(tài)性。采用SPSS16.0進(jìn)行數(shù)據(jù)統(tǒng)計(jì)，，用擬合優(yōu)度χ2檢驗(yàn)進(jìn)行Hardy-Weiberg平衡檢測(cè)，基因型、等位基因分布采用χ2檢驗(yàn)，肺結(jié)核環(huán)境因素及其與基因多態(tài)性的相互作用采用單因素分析或多因素Logistic回歸分析，以P＜0.05表示差異具有顯著性意義。 結(jié)果：對(duì)病例組993例，對(duì)照組880例進(jìn)行VDR基因多態(tài)性分型結(jié)果，兩位點(diǎn)均符合Hardy-Weiberg平衡。PCR-RFLP結(jié)果的多因素Logistic回歸分析顯示，VDR-(TT+Tt)、-(FF+ff)基因型在病例對(duì)照組中的分布差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）；男性VDR-TT基因型、回族VDR-(FF+ff)基因型在病例對(duì)照組中的分布具有差異顯著性。結(jié)論：VDR基因多態(tài)性與寧夏回人群肺結(jié)核易感性有關(guān)。VDR-(TT+Tt)、男性 VDR-TT基因型可能是肺結(jié)核病的保護(hù)因素；VDR-(FF+ff)、回族VDR-(FF+ff)基因型可能是肺結(jié)核發(fā)病的危險(xiǎn)因素。
[Abstract]:Objective: To explore the relationship between vitamin D receptor (VDR) gene polymorphism and susceptibility to pulmonary tuberculosis in the Ningxia Hui Autonomous Region population by case-control study.
Methods: the case group was from the Ningxia Hui Autonomous Region. In 2010, the pulmonary tuberculosis patients were confirmed by the WHO pulmonary tuberculosis diagnosis standard by all the experts in the hospital. The patients with the same sex, the same nationality, the same age, the living environment matched the patients with no pulmonary tuberculosis as the control group. The case control group collected 2-5mL venous blood and pulmonary tuberculosis. The environmental factors were investigated by a questionnaire. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of VDR-rs731236 and rs2228570 loci. SPSS16.0 was used for data statistics, Hardy-Weiberg balance test was performed with the goodness of fit chi 2 test. The genotype, allele distribution was tested by chi 2 test, pulmonary tuberculosis The interaction between environmental factors and genetic polymorphisms was analyzed by univariate analysis or multivariate Logistic regression analysis. The difference was significant in P < 0.05.
Results: 993 cases in the case group and 880 cases in the control group were the results of the polymorphism of VDR gene, and the multiple factor Logistic regression analysis of the two loci was consistent with the Hardy-Weiberg balance.PCR-RFLP results. The distribution of VDR- (TT+Tt) and (FF+ff) genotype in the case control group was statistically significant (P < 0.05); the male VDR-TT genotype, the VDR- of the Hui nationality was VDR-. (FF+ff) genotype distribution in the case-control group was significantly different. Conclusion: VDR gene polymorphism is associated with susceptibility to tuberculosis in Ningxia population.VDR- (TT+Tt), male
VDR-TT genotype may be a protective factor for pulmonary tuberculosis; VDR- (FF+ff) and Hui VDR- (FF+ff) genotype may be a risk factor for pulmonary tuberculosis.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R521

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王紀(jì)祥;鮑方進(jìn);張根友;楊建安;汪愛民;張永忠;;安徽省第五次結(jié)核病流行病學(xué)調(diào)查報(bào)告[J];安徽預(yù)防醫(yī)學(xué)雜志;2011年03期

2 郗永義,鄭秀芬,葉健,霍賽虎,張健,陳帥鋒;維生素D受體研究進(jìn)展[J];大連醫(yī)科大學(xué)學(xué)報(bào);2004年03期

3 周文娣;張佩斌;;維生素D受體及維生素D作用進(jìn)展[J];中國兒童保健雜志;2008年05期

4 徐波,王保捷;維生素D受體基因多態(tài)性研究進(jìn)展[J];法醫(yī)學(xué)雜志;2005年01期

5 王欣;翁少凡;祖愛華;郭雁飛;周婷;陳衛(wèi)紅;;肺結(jié)核危險(xiǎn)因素病例對(duì)照研究[J];工業(yè)衛(wèi)生與職業(yè)病;2011年04期

6 李曉鳳;結(jié)核病流行狀況及控制措施[J];中國熱帶醫(yī)學(xué);2005年05期

7 楊本付;韓長磊;;維生素D受體基因多態(tài)性與肺結(jié)核關(guān)系的Meta分析[J];中國熱帶醫(yī)學(xué);2006年08期

8 馮福民;郭梅;郝金奇;陳怡;孫淑豐;鄭國穎;陳剛;;維生素D受體基因多態(tài)性與漢族人肺結(jié)核發(fā)病的關(guān)系[J];山東醫(yī)藥;2009年45期

9 梅建;結(jié)核病流行現(xiàn)狀與控制策略[J];上海預(yù)防醫(yī)學(xué)雜志;2003年03期

10 熊宇;李革;;肺結(jié)核的遺傳易患性研究[J];現(xiàn)代預(yù)防醫(yī)學(xué);2007年21期

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