本文選題：N-乙酰半胱氨酸 + 慢性阻塞性肺疾病��； 參考：《動物醫(yī)學(xué)進展》2014年06期

【摘要】：為觀察N-乙酰半胱氨酸(NAC)抗氧化作用對慢性阻塞性肺疾病(COPD)模型大鼠血管活性物質(zhì)的影響,將100只清潔級Wistar大鼠隨機分為對照組、模型組、NAC低劑量組、中劑量、高劑量5組,每組20只。模型組采用氣管注入脂多糖(LPS)和香煙染毒的復(fù)合方法造模。在模型組基礎(chǔ)上,低劑量組、中劑量、高劑量組分別按26.32、52.64、105.28mg/kg NAC灌胃,連續(xù)2周。檢測丙二醛(MDA)、超氧化物歧化酶(SOD)、血管緊張素Ⅱ(AngⅡ)、活性氧(ROS)、內(nèi)皮素(ET)、一氧化氮(NO)及肺組織核轉(zhuǎn)錄因子κB(NF-κB)的變化,分析AngⅡ與ROS、NF-κB的相關(guān)性。結(jié)果顯示,與對照組比較,模型組AngⅡ、ROS、ET、MDA水平均顯著升高而NO、SOD水平極顯著下降(P0.01),NF-κB活性極顯著升高(P0.01)。與模型組比較,低劑量組NO水平升高而ET水平顯著下降(P0.05);中劑量組AngⅡ、ROS、ET、MDA水平下降或顯著下降而NO、SOD水平均升高(P0.05或P0.01),NF-κB活性顯著下降(P0.05);高劑量組AngⅡ、ROS、ET、MDA水平均顯著下降而NO、SOD水平均極顯著升高(P0.01),NF-κB活性極顯著下降(P0.01)。AngⅡ水平與NF-κB活性,AngⅡ水平與ROS水平均呈顯著正相關(guān)關(guān)系(P0.01)。表明NAC劑量依賴性改善COPD病理變化,推測其可能是通過降低AngⅡ水平,從而減少ROS的產(chǎn)生,進而抑制NF-κB活化過程而起效。
[Abstract]:In order to observe the effect of N-acetylcysteine (NAC) on vasoactive substances in rats with chronic obstructive pulmonary disease (COPD) model, 100 clean grade Wistar rats were randomly divided into control group, low dose group, middle dose group and high dose group. There were 20 rats in each group. The model group was made by endotracheal injection of lipopolysaccharide (LPS) and cigarette exposure. On the basis of the model group, the low dose group, middle dose group and high dose group were perfused with 26.32 渭 g / kg 52.64105.28 mg / kg NAC for 2 weeks respectively. The changes of malondialdehyde (MDA), superoxide dismutase (SOD), angiotensin 鈪，

本文編號：1916933