發(fā)布時(shí)間：2018-05-03 18:22

  本文選題：肺纖維化 + 轉(zhuǎn)化生長(zhǎng)因子β(TGF-β)　； 參考：《細(xì)胞與分子免疫學(xué)雜志》2017年04期

【摘要】：目的研究阿爾法骨化醇聯(lián)合地塞米松對(duì)肺纖維化影響。方法 C57BL/6小鼠40只,隨機(jī)分為正常對(duì)照組、肺纖維化組、阿爾法骨化醇組、地塞米松組、地塞米松聯(lián)合阿爾法骨化醇組。正常對(duì)照組予以0.5μL/g生理鹽水一次性氣管注射,同時(shí)每天胃內(nèi)灌注生理鹽水0.2 m L;其他組予以0.5μL/g博來(lái)霉素溶液一次性氣管注射,肺纖維化組每天給予生理鹽水0.2 m L灌胃,地塞米松組予以地塞米松[0.5 mg/(kg·d)]灌胃,阿爾法骨化醇組予以阿爾法骨化醇[0.5μg/(kg·d)]灌胃,地塞米松聯(lián)合阿爾法骨化醇組給予地塞米松[0.5 mg/(kg·d)]聯(lián)合阿爾法骨化醇[0.5μg/(kg·d)]灌胃,21 d后處死。行HE及Masson染色觀察肺組織病理變化,收集血清檢測(cè)各組羥脯氨酸(Hyp)值,收集支氣管肺泡灌洗液(BALF),沉淀重懸行細(xì)胞計(jì)數(shù),上清液行ELISA檢測(cè)白細(xì)胞介素1β(IL-1β)、IL-6、腫瘤壞死因子α(TNF-α)及轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)。結(jié)果阿爾法骨化醇聯(lián)合地塞米松組IL-1β、IL-6、TNF-α及TGF-β1,細(xì)胞計(jì)數(shù)、Sazpiel評(píng)分、Hyp值均低于除對(duì)照組外其他各組,其肺泡炎情況及纖維沉積也均低于除對(duì)照組外其他各組。結(jié)論阿爾法骨化醇聯(lián)合地塞米松對(duì)肺纖維療效優(yōu)于地塞米松及阿爾法骨化醇單藥療效,其機(jī)制可能與抑制TGF-β1、炎性因子相關(guān)。
[Abstract]:Objective to study the effect of Alpha-ositol combined with dexamethasone on pulmonary fibrosis. Methods Forty C57BL/6 mice were randomly divided into normal control group, pulmonary fibrosis group, Alpha-ositol group, dexamethasone group and dexamethasone combined with alpha osciol group. In the control group, 0.5 渭 L / g saline was injected into the trachea and 0.2 mL of saline was injected into the stomach every day, while the other group was given 0.5 渭 L / g bleomycin solution once a day, and the pulmonary fibrosis group was given 0.2 mL of normal saline every day. The dexamethasone group was given dexamethasone [0.5 mg/(kg d], the alpha osciol group [0.5 渭 g/(kg d], the dexamethasone combined with alpha ossification group [0.5 mg/(kg d] and alpha oscisol [0.5 渭 g/(kg d] for 21 days. The pathological changes of lung tissue were observed by HE and Masson staining. The serum levels of hydroxyproline were collected. The bronchoalveolar lavage fluid (BALF) was collected. IL-6, TNF- 偽, TGF- 尾 _ 1 and TGF- 尾 _ 1 of TGF- 尾 _ (1) and TGF- 尾 _ (1) were detected by ELISA in supernatant. Results the levels of IL-1 尾 IL-6 TNF- 偽 and TGF- 尾 1, cell count and Sazpiel score (Hyp) were lower in combination with dexamethasone than those in the control group, and the alveolitis and fiber deposition were also lower than those in other groups except the control group. Conclusion Alpha-ositol combined with dexamethasone is more effective than dexamethasone and alpha-ositol in the treatment of lung fiber, and its mechanism may be related to the inhibition of TGF- 尾 _ 1 and inflammatory factor.
【作者單位】： 西南醫(yī)科大學(xué)呼吸內(nèi)科;四川大學(xué)華西臨床醫(yī)學(xué)院呼吸內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金(81170032) 四川省自然科學(xué)基金(06085) 四川省人事廳資金資助項(xiàng)目(11017)
【分類號(hào)】：R563
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本文編號(hào)：1839577