發(fā)布時間：2018-05-03 01:15

  本文選題：ALI/ARDS + 消退素D1　； 參考：《華中科技大學(xué)》2013年碩士論文

【摘要】：目的：本研究的目的是探討消退素D1對LPS引起的急性肺損傷小鼠肺部緊密連接蛋白破壞的保護作用及機制。 方法：以小鼠氣管滴注脂多糖（3mg/kg）誘導(dǎo)急性肺損傷模型，采用隨機數(shù)字表法，將SPF級雄性小鼠（8-10w）隨機分為6組（n=10）：（1）空白對照組(Control組),（2）LPS組；（3）LPS+RvD1組,（4）RvD1組,（5）LPS+RvD1+Znpp組，（6）LPS+Znpp組。消退素D1生理鹽水稀釋后5μg/kg，于LPS吸入前30min尾靜脈注射，Znpp溶于二甲基亞砜50mg/kg于消退素30min腹腔注射，其他組給予等體積0.9%無菌生理鹽水，LPS3mg/kg經(jīng)切開的氣管吸入后觀察24h，深麻醉下放血處死小鼠，單肺灌洗，取肺組織。石蠟切片及HE染色制作肺組織病理切片觀察；55℃干烤5d，檢測濕干重比(w/d比)；緊密連接蛋白ZO-1和Occludin蛋白及mRNA表達水平；HO-1mRNA及蛋白表達水平；肺組織細胞凋亡率(TUNEL)；SABC免疫熒光法觀察肺組織細胞間的緊密連接。 結(jié)果：病理切片顯示，LPS+RvD1組肺組織損傷明顯比LPS組減輕。LPS+RvD1組的肺濕干比明顯小于同時間LPS組（P＜0.05）；與正常組比，LPS組緊密連接蛋白ZO-1和Occludin蛋白及mRNA表達水平明顯降低（P＜0.01，P＜0.05），TUNEL示肺組織細胞凋亡率明顯增加，，HO-1mRNA及蛋白表達水平稍有增加具有統(tǒng)計學(xué)差異(P＜0.05)；與LPS組比較，LPS+RvD1組上述指標中肺組織緊密連接蛋白ZO-1和Occludin蛋白及mRNA表達水平均升高（P＜0.05），HO-1mRNA及蛋白表達水平升高明顯(P＜0.01)，肺組織細胞凋亡率明顯降低；LPS+RvD1+Znpp組與LPS組比較僅有HO-1蛋白水平和mRNA表達明顯下降(P＜0.01)，其他指標無統(tǒng)計學(xué)意義(P＞0.05)，與LPS+RvD1組比較，HO-1蛋白水平和mRNA表達下降，ZO-1和Occludin蛋白及mRNA表達水平降低(P＜0.05)，細胞凋亡增加；另外，Control組與RvD1組無統(tǒng)計學(xué)差異，LPS組與LPS+Znpp組無統(tǒng)計學(xué)差異，但LPS+Znpp組HO-1的蛋白和mRNA表達水平明顯降低(P＜0.01)。免疫熒光顯示LPS+RvD1組緊密連接蛋白表達量明顯多于LPS組，細胞屏障破壞明顯減少。 結(jié)論：消退素D1可以降低肺濕干比，減輕肺病理變化，減輕肺水腫；消退素D1預(yù)處理可以上調(diào)HO-1表達，減少緊密連接蛋白破壞，減少肺組織細胞凋亡，從而減輕急性肺損傷引起的肺水腫。
[Abstract]:Aim: to investigate the protective effect and mechanism of vanishing hormone D1 on lung tight junction protein damage induced by LPS in mice. Methods: acute lung injury model was induced by endotracheal instillation of lipopolysaccharide (3 mg / kg) in mice. SPF grade male mice were randomly divided into 6 groups for 8 to 10 weeks) (control group, lipopolysaccharide group, lipopolysaccharide D1 group, lipopolysaccharide group, RvD1 Znpp group, and control group. 5 渭 g / kg of attenuated D1 saline was injected intraperitoneally with 30min dissolved in 50mg/kg of dimethyl sulfoxide (50mg/kg) and intraperitoneally injected with dimethylsulfoxide (50mg/kg) before LPS inhalation. The other groups were treated with 0.9% aseptic saline (LPS 3 mg / kg) for 24 hours after tracheotomy. The mice were sacrificed under deep anesthesia. The mice were perfused with a single lung and the lung tissues were taken out. Paraffin sections and HE staining were used to make pathological sections of lung tissues for 5 days at 55 鈩

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