本文關(guān)鍵詞：熱休克蛋白70在氣道黏液高分泌中的作用，，由筆耕文化傳播整理發(fā)布。

熱休克蛋白70在氣道黏液高分泌中的作用及機(jī)制

Role and mechanism of heat shock protein 70 in airway hypersecretion

[1] [2] [3] [4] [5]

Zhou Zhiyu, Zhang Ting, Zhou Xiangdong, Li Qi, Liu Chunyi.（Department of Respiratory Medicine, Second Affiliated Hospital, Chongqing University Medical Science, Chongqing 400010

重慶醫(yī)科大學(xué)附屬第二醫(yī)院呼吸內(nèi)科,400010

文章摘要：目的 探討熱休克蛋白70 （HSP70）在氣道黏液高分泌中的作用及機(jī)制.方法 用8％香煙提取物（CSE）處理人氣道A549細(xì)胞后,分別給予HSP70抗體阻斷劑、c-Jun氨基末端激酶（JNK）特異性抑制劑SP600125進(jìn)行干預(yù).實(shí)驗(yàn)共分4組：空白對(duì)照組（無(wú)血清培養(yǎng)基培養(yǎng)）、CSE刺激組（加入8％ CSE培養(yǎng)24 h）、HSP70抗體組（HSP70單抗預(yù)處理30 min,再給予8％ CSE繼續(xù)培養(yǎng)24h）、SP600125組（加入JNK特異性抑制劑SP600125 30 μmol/L預(yù)處理30 min,再給予8％ CSE繼續(xù)培養(yǎng)24 h）.酶聯(lián)免疫吸附（ELISA）法檢測(cè)各組中MUC5AC蛋白的相對(duì)表達(dá)量,逆轉(zhuǎn)錄-PCR檢測(cè)MUC5AC mRNA的相對(duì)表達(dá)量,Western印跡法檢測(cè)HSP70水平,Western印跡法檢測(cè)JNK及激活蛋白-1（主要為c-Jun）磷酸化水平.結(jié)果 CSE刺激組細(xì)胞MUC5AC蛋白、MUC5AC mRNA、p-JNK、c-Jun、p-c-Jun相對(duì)表達(dá)量（0.52±0.07、0.64±0.11、0.73±0.06、0.67±0.10、0.67±0.09）均顯著高于空白對(duì)照組（0.26±0.10、0.28±0.06、0.30±0.05、0.30±0.08、0.36±0.08）、HSP70抗體組（0.30±0.12、0.29±0.09、0.34±0.06、0.47±0.19、0.39±0.13）和SP600125組（0.38±0.06、0.31±0.14、0.39±0.04、0.44±0.12、0.48±0.11）,HSP70 （0.75±0.09）也顯著高于空白對(duì)照組（0.29±0.03）、HSP70抗體組（0.40±0.11）（均P＜0.05）.結(jié)論 HSP70可能通過(guò)JNK/激活蛋白-1信號(hào)通路上調(diào)氣道A549細(xì)胞中MUC5AC的表達(dá).

Abstr：Objective To explore the role and mechanism of heat shock protein 70 （HSP70） in airway hypersecretion.Methods After a stimulation of 8% cigarette smoke extract （CSE）,airway cells A549 were treated with HSP70 antibody and c-Jun N-terminal kinase （JNK） specific inhibitor SP600125 respectively.And the cells were divided into 4 groups of blank contrast （serum-free medium）,CSE stimulation （8% CSE for 24 h）,HSP70 antibody （30-min pre-treatment of HSP70 antibody and culturing in 8% CSE for 24 h） and SP600125 （30-min pre-treatment of SP600125 30 μmol/L and culturing in 8% CSE for 24 h）.The relative expression levels of MUC5AC protein in various groups were determined by enzymelinked immunosorbent assay （ELISA）.And the relative transcription level of MUC5AC mRNA was detected by reverse transcription-polymerase chain reaction （PCR） while the synthesized levels of HS70 as well as the phosphorylation levels of JNK and activated protein-1 （mostly c-Jun） were measured by Western blot.Results As compared with those in blank contrast group （0.26 ± 0.10,0.28 ± 0.06,0.30 ± 0.05,0.30±0.08,0.36±0.08）,HSP70 antibody group （0.30 ±0.12,0.29 ±0.09,0.34 ±0.06,0.47 ± 0.19,0.39 ±0.13） and SP600125 group （0.38 ±0.06,0.31 ±0.14,0.39 ±0.04,0.44 ±0.12,0.48 ± 0.11）,the relative expression levels of MUC5AC protein and mRNA,phosphorylation JNK （p-JNK）,c-Jun and p-c-Jun （0.52 ±0.07,0.64 ±0.11,0.73 ±0.06,0.67 ±0.10,0.67 ±0.09） significantly increased in CSE stimulation group （all P 〈 0.05）.And the synthesis levels of HSP70 （0.75 ± 0.09） in CSE stimulation group increased than blank contrast group （0.29 ± 0.03） and HSP70 antibody group （0.40 ± 0.11） （all P 〈 0.05）.Conclusion HSP70 may enhance the expression of MUC5AC in bronchial epithelial A549 cells via a

文章關(guān)鍵詞：

Keyword：:HSP70 heat-shock proteins Mucins Cigarette smoke extract Activated protein

課題項(xiàng)目：國(guó)家自然科學(xué)基金（81370111）

作者信息：會(huì)員可見

  本文關(guān)鍵詞：熱休克蛋白70在氣道黏液高分泌中的作用，由筆耕文化傳播整理發(fā)布。