發(fā)布時(shí)間：2018-04-22 22:15

  本文選題：地塞米松 + 支氣管哮喘��； 參考：《山西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的： 探討支氣管哮喘大鼠氣道重塑與其血清白介素-8（interleukin-8）、肺組織表皮生長因子（Epidermal Growth Factor,EGF）表達(dá)的關(guān)系及地塞米松早、晚期的干預(yù)效果。 方法： 40只清潔級(jí)wistar雄性大鼠，隨機(jī)分為4組：模型組（N組）、對(duì)照組（C組）、地塞米松早期干預(yù)組(E組)、地塞米松晚期干預(yù)組(L組)，每組各10只，用10%卵蛋白(Ovalbumin，，OVA)抗原混懸液腹腔注射致敏，1%卵清白蛋白霧化誘喘，以制備大鼠哮喘氣道重塑模型。其中地塞米松早、晚期干預(yù)組在不同時(shí)期給予地塞米松進(jìn)行干預(yù)。而對(duì)照組大鼠用生理鹽水代替卵蛋白致敏和誘喘。ELISA法測(cè)血清中IL-8含量，肺組織切片HE染色，計(jì)算機(jī)圖像分析氣道形態(tài)學(xué)參數(shù)，衡量氣道重塑程度，免疫組化法測(cè)定肺組織中EGF含量。數(shù)據(jù)分析用SPSS17.0統(tǒng)計(jì)軟件處理。 結(jié)果： 除C組外，其余各組均有氣道重塑的病理改變；與C組大鼠相比較，各組大鼠氣道重塑程度較重、血清IL-8濃度升高、肺組織EGF表達(dá)上調(diào)(P0.05)；與N組大鼠相比較，各組大鼠均有氣道重塑程度較輕、血清IL-8濃度下降、肺組織EGF表達(dá)下調(diào)(P0.05)；與E組相比較，L組和N組大鼠氣道重塑程度較重、血清IL-8濃度升高、肺組織EGF表達(dá)上調(diào)(P0.05)；另外，各組大鼠血清IL-8濃度、肺組織EGF表達(dá)與氣道重塑程度相關(guān)(P0.001)。 結(jié)論： 在大鼠支氣管哮喘氣道重塑模型中證實(shí)地塞米松可以有效抑制氣道重塑，且早期應(yīng)用更能盡早阻斷其病程的進(jìn)展；同時(shí)地塞米松可以下調(diào)IL-8、EGF的表達(dá)，而且IL-8、EGF含量與氣道重塑程度呈正相關(guān)，由此推測(cè)地塞米松可能是通過抑制IL-8、EGF表達(dá)的途徑來干預(yù)氣道重塑，另外IL-8、EGF也可能作為衡量氣道重塑程度重要指標(biāo)之一。
[Abstract]:Objective: To investigate the relationship between airway remodeling and the expression of interleukin-8 in serum and epidermal Growth factor-EGFin in lung tissue of asthmatic rats and the effect of dexamethasone on the early and late stage of dexamethasone. Methods: Forty clean grade wistar male rats were randomly divided into four groups: model group (n group), control group (group C), dexamethasone early intervention group (group E) and dexamethasone late intervention group (group L) with 10 rats in each group. The airway remodeling model of asthmatic rats was established by injecting 10% ovalbumin OVA antigen suspension intraperitoneally with 1% ovalbumin and 1% ovalbumin to induce asthma. Dexamethasone in early and late intervention group was given dexamethasone in different periods. In the control group, the rats were sensitized by normal saline instead of ovalbumin and panting. Elisa was used to measure the content of IL-8 in serum, HE staining in lung tissue sections, morphological parameters of airway in computer image analysis, and the degree of airway remodeling. The content of EGF in lung tissue was determined by immunohistochemical method. Data analysis is processed by SPSS17.0 statistical software. Results: There were pathological changes of airway remodeling in all groups except group C. compared with group C, the degree of airway remodeling was heavier, the concentration of serum IL-8 was higher, the expression of EGF in lung tissue was up-regulated (P0.05), and compared with group N, the degree of airway remodeling in each group was higher than that in group C. Compared with group E, the degree of airway remodeling in group L and group N was more severe, the concentration of serum IL-8 was higher, and the expression of EGF in lung tissue was up-regulated (P 0.05). The concentration of serum IL-8 and the expression of EGF in lung tissue were correlated with the degree of airway remodeling in each group (P 0.001). Conclusion: In rat bronchial asthma airway remodeling model, dexamethasone can effectively inhibit airway remodeling, and early application of dexamethasone can block the progression of airway remodeling, and dexamethasone can down-regulate the expression of IL-8 and EGF. Furthermore, the content of IL-8 EGF was positively correlated with the degree of airway remodeling, which suggested that dexamethasone might interfere with airway remodeling by inhibiting the expression of IL-8 EGF, and IL-8 EGF might also be one of the important indexes to measure the degree of airway remodeling.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R562.25

【共引文獻(xiàn)】

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