本文選題：肺結(jié)核　切入點(diǎn)：肺部炎癥　出處：《中國(guó)人獸共患病學(xué)報(bào)》2014年07期

【摘要】：目的探索應(yīng)用蛋白質(zhì)指紋圖譜技術(shù)于菌陰肺結(jié)核與肺炎的鑒別診斷。方法從本院臨床病例中,選擇菌陰肺結(jié)核和肺炎患者及健康者各60例,應(yīng)用表面加強(qiáng)激光解吸電離飛行時(shí)間質(zhì)譜技術(shù)(SELDI/ToF-Ms)和蛋白芯片技術(shù)檢測(cè)血清蛋白,并應(yīng)用Ciphergen蛋白芯片3.1.1軟件進(jìn)行比較,分析其相關(guān)蛋白峰值并進(jìn)行統(tǒng)計(jì)學(xué)處理。結(jié)果對(duì)180例菌陰肺結(jié)核、肺炎患者、健康者的血清蛋白指紋圖譜數(shù)據(jù)進(jìn)行比較,發(fā)現(xiàn)有5個(gè)蛋白峰(1 028.49、4 796.56、7 564.77、8 048.02、11 526.75m/z)存在顯著的差異,有統(tǒng)計(jì)學(xué)意義(P0.01)。由這5個(gè)蛋白峰組成的診斷模型鑒別診斷菌陰肺結(jié)核與肺炎的總有效率84.2%(101/120),敏感性與特異性分別為82.5%(52/63),85.9%(49/57);陽(yáng)性預(yù)測(cè)值86.7%(52/60),陰性預(yù)測(cè)值為81.7%(49/60)。診斷模型在判別肺炎、菌陰肺結(jié)核患者與健康者之間,總有效率達(dá)89.4%(161/180),特異性為100%(60/60),靈敏度為84.2%(101/120),陽(yáng)性預(yù)測(cè)值100%(101/101),陰性預(yù)測(cè)值75.9%(60/79)。結(jié)論蛋白質(zhì)指紋圖譜技術(shù)具有方法簡(jiǎn)便、檢測(cè)快速,標(biāo)本用量少的優(yōu)點(diǎn),是篩選結(jié)核病特異性標(biāo)志物的有效手段,通過(guò)蛋白質(zhì)指紋圖譜技術(shù)檢測(cè),發(fā)現(xiàn)了具有良好鑒別診斷的"診斷模型"。
[Abstract]:Objective to explore the application of protein fingerprinting in the differential diagnosis of bacterial negative pulmonary tuberculosis and pneumonia. Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI / ToF-Ms) and protein chip technique were used to detect serum protein and compared with Ciphergen protein chip 3.1.1 software. Results the serum protein fingerprint data of 180 patients with pulmonary tuberculosis, pneumonia and healthy persons were compared. It was found that there were significant differences in 5 protein peaks (1 028.49 / 4 7796.56 / 7 / 8 048.02.02 / 11 526.75m/ z). The total effective rate of differential diagnosis of negative pulmonary tuberculosis and pneumonia was 84.2 / 101 / 120, the sensitivity and specificity were 82.5 / 52 / 63 and 85.9 / 57, respectively; the positive predictive value was 86.752 / 60 and the negative predictive value was 81.77.The diagnostic model was discriminating pneumonia. The total effective rate was 89.4% 16.1% 180%, the specificity was 100% 60 / 60%, the sensitivity was 84.2% 101 / 120, the positive predictive value was 100% 101 / 101%, the negative predictive value was 75.9% / 790.Conclusion protein fingerprinting technique has the advantages of simple, rapid detection and low sample consumption. It is an effective method for screening specific markers of tuberculosis. A "diagnostic model" with good differential diagnosis has been found by detecting protein fingerprinting.
【作者單位】： 福建省福州肺科醫(yī)院/福建醫(yī)科大學(xué)臨床教學(xué)醫(yī)院;
【基金】：福建省自然科學(xué)基金(No.2012J01426)資助~~
【分類(lèi)號(hào)】：R521
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本文編號(hào)：1677376