本文選題：缺氧　切入點(diǎn)：慢性阻塞性肺疾病　出處：《青海大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討不同海拔大鼠慢性阻塞性肺疾�。–OPD）模型全身效應(yīng)的差別及與血漿中腫瘤壞死因子α（TNF-α）、白細(xì)胞介素6（IL-6）及白細(xì)胞介素10（IL-10）的水平的關(guān)系，進(jìn)而了解高原缺氧對COPD大鼠全身炎癥反應(yīng)的影響。 方法：以腹腔注射香煙提取物法建立大鼠COPD模型。50只SD大鼠隨機(jī)分為對照組、中海拔鹽水組、中海拔COPD組（西寧，海拔2260m）和高海拔鹽水組、高海拔COPD組（低壓氧艙，海拔5500m），，觀察大鼠進(jìn)食、飲水、活動(dòng)量、情緒及體重，應(yīng)用雙抗體夾心法（ELISA）方法測定SD大鼠血漿中TNF-α、IL-6及IL-10的水平。 結(jié)果：不同組別大鼠全身表現(xiàn)不同，中海拔COPD組較中海拔鹽水組及對照組活動(dòng)量減少，易激惹；高海拔鹽水組較中海拔鹽水組，高海拔COPD組較中海拔COPD組飲食、飲水量及活動(dòng)量明顯減少，且易激惹。高海拔鹽水組體重低于中海拔鹽水組及對照組（P0.01），高海拔COPD組體重低于中海拔COPD組（P0.01）。高海拔鹽水組TNF-α、IL-6水平明顯高于中海拔鹽水組和對照組（P0.05）；高海拔COPD組TNF-α、IL-6水平高于中海拔COPD組（P0.05），同海拔COPD組高于鹽水組（P0.05）。高海拔鹽水組IL-10高于中海拔鹽水組及對照組（P0.01） 結(jié)論：1.高原缺氧促進(jìn)健康大鼠血漿中TNF-α、IL-6的分泌并可造成全身效應(yīng)；2.高原缺氧加劇了COPD大鼠的全身炎癥反應(yīng)和全身效應(yīng)；3.IL-10在COPD大鼠中減弱了抑制炎癥因子、抑制炎癥反應(yīng)的作用。
[Abstract]:Objective: To investigate the effects of chronic obstructive pulmonary disease in rats at different altitudes (COPD) model and the difference between the systemic effects and plasma tumor necrosis factor alpha (TNF- alpha), interleukin 6 (IL-6) and interleukin 10 (IL-10) the level of the relationship, and to understand the effects of hypoxia on systemic inflammatory reaction COPD rats.
Methods: intraperitoneal injection of cigarette smoke extract method to establish a rat model of COPD.50 SD rats were randomly divided into control group, saline group in altitude, altitude COPD group (Xining, 2260m above sea level) and high altitude saline group, COPD group (high altitude hypobaric chamber, altitude 5500m), rats eating, drinking water that activity, mood and body. The application of double antibody sandwich enzyme-linked immunosorbent assay (ELISA) method for the determination of TNF- alpha SD in rat plasma, IL-6 and IL-10 levels.
Results: all rats in different groups were different in the elevation of COPD group compared with the saline group and control group in the altitude of reduced activity, irritability; high altitude saline group than in saline group at high altitude, COPD group than in COPD group at eating, drinking and activity were significantly decreased, and irritability. High altitude elevation weight lower than the saline group saline group and control group (P0.01), high altitude group COPD COPD group weighed less than in altitude (P0.01). The high altitude control group TNF- alpha, IL-6 level was significantly higher than that in soil saline group and control group (P0.05); high altitude group COPD TNF- alpha, IL-6 level is higher than that in the elevation of COPD group (P0.05), with an elevation of COPD group was higher than that of saline group (P0.05). The high altitude is higher than that of saline group IL-10 elevation saline group and control group (P0.01)
Conclusion: 1.. Hypoxia at high altitude promotes the secretion of TNF- and IL-6 in healthy rats, and can cause systemic effects. 2., hypoxia at high altitude aggravates the systemic inflammatory response and systemic effect in COPD rats. 3.IL-10 weakens the inhibition of inflammatory factors and inhibits inflammatory response in COPD rats.

【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R563

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