發(fā)布時(shí)間：2018-03-08 02:22

  本文選題：二硫代氨基吡咯烷　切入點(diǎn)：核因子-κB　出處：《醫(yī)藥導(dǎo)報(bào)》2014年03期 　論文類型：期刊論文

【摘要】：目的探討二硫代氨基吡咯烷(PDTC)對(duì)內(nèi)毒素(LPS)誘導(dǎo)急性肺損傷(ALI)大鼠肺組織的保護(hù)作用及其機(jī)制。方法將72只Wistar大鼠隨機(jī)分為對(duì)照組(C組),LPS組(L組),PDTC+LPS組(P組)。各組分為1,3,6,12 h亞組,每組6只。L組和P組制作內(nèi)毒素性肺損傷模型。P組腹腔注射LPS前30 min尾靜脈注射PDTC,120 mg·kg-1,L組和C組給予等體積0.9%氯化鈉溶液。觀察大鼠肺組織病理變化,肺組織濕/干質(zhì)量比(W/D)變化,RT-PCR法檢測(cè)大鼠肺組織細(xì)胞間黏附分子-1 mRNA(ICAM-1mRNA)的表達(dá),放射免疫法檢測(cè)大鼠血清腫瘤壞死因子α(TNF-α)、白細(xì)胞介素6(IL-6)含量變化。結(jié)果病理切片顯示,與C組比較,L組肺組織結(jié)構(gòu)破壞嚴(yán)重,W/D明顯增大,ICAM-1mRNA在各時(shí)間點(diǎn)表達(dá)明顯增強(qiáng)(P0.05),血清TNF-α、IL-6含量在1,3,6 h增高(P0.05)。與L組比較,P組肺組織結(jié)構(gòu)破壞明顯減輕,W/D減小(P0.05),ICAM-1mRNA表達(dá)明顯減少(P0.05),血清TNF-α、IL-6含量1,3,6 h降低(P0.05或P0.01)。結(jié)論 PDTC能明顯抑制ICAM-1mRNA表達(dá),降低TNF-α和IL-6含量,PDTC可能對(duì)ALI大鼠有保護(hù)作用。
[Abstract]:Objective to investigate the protective effect of dithioaminopyrrolidine on the lung tissue of rats with acute lung injury induced by lipopolysaccharide (LPS) and its mechanism. Methods 72 Wistar rats were randomly divided into control group (group C) and control group (group C). Endotoxin-induced lung injury model was established in each group (n = 6) and group P (n = 6). Group P was injected intraperitoneally with PDT Con 120mg 路kg ~ (-1) L and group C (n = 30) 30 min before intraperitoneal injection with sodium chloride solution of equal volume (0.9% mg 路kg ~ (-1)). The pathological changes of lung tissue in rats were observed. Lung tissue wet / dry weight ratio: reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of intercellular adhesion molecule-1 mRNA (ICAM-1mRNAs), and radioimmunoassay (RIA) was used to detect the contents of tumor necrosis factor- 偽 (TNF- 偽) and interleukin-6 (IL-6) in rat lung tissue. Compared with group C, the expression of ICAM-1 mRNA and the level of TNF- 偽 IL-6 in serum increased significantly at each time point in group C and group C, respectively. Compared with group L, the destruction of lung tissue structure in group C significantly decreased the expression of ICAM-1 mRNA in lung tissue, and the expression of ICAM-1 mRNA decreased significantly in group A (P 0.05) compared with that in group L (P < 0.05), and the expression of ICAM-1 mRNA was significantly reduced in group C than in group C (P < 0.05), and the level of TNF- 偽 -IL-6 in serum was significantly increased at 6 h after treatment. The level of TNF- 偽 IL-6 in serum decreased by P0.05 or P0.01 for 6 h. Conclusion PDTC can significantly inhibit the expression of ICAM-1mRNA. The decrease of TNF- 偽 and IL-6 may have protective effect on ALI rats.
【作者單位】： 山東省勝利油田中心醫(yī)院麻醉科;蘭州大學(xué)第一醫(yī)院麻醉科;
【分類號(hào)】：R563.8

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本文編號(hào)：1581999