本文選題：熱煙霧吸入性肺損傷　切入點：hMSCs　出處：《遼寧醫(yī)學(xué)院》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 1、探討經(jīng)氣管移植人的骨髓間充質(zhì)干細胞對熱煙霧吸入性肺損傷大鼠的作用； 2、探討經(jīng)尾靜脈注入人骨髓間充質(zhì)干細胞對熱煙霧吸入性肺損傷大鼠的作用； 3、研究不同移植途徑對干細胞發(fā)揮作用的影響。 方法 1、自行研制熱煙霧發(fā)生裝置，模擬火災(zāi)現(xiàn)場熱煙霧環(huán)境。將56只雄性健康Wistar大鼠隨機數(shù)字表法分為正常對照組(8只)、熱煙霧吸入致傷組(24只)和經(jīng)氣管移植hMSCs治療組（24只），后兩組再按照傷后時間點分為3個亞組（2h、4h、8h）。對照組在8h之后活殺，熱煙霧吸入致傷組和經(jīng)氣管移植hMSCs治療組分別于致傷后2h,4h,8h活殺。檢測血清及支氣管肺泡灌洗液（BronchoalveolarLavage Fluid BALF）中腫瘤壞死因子-α（Tumor Necrosis Factor-α TNF-α）、白介素-6（Interleukin-6,IL-6）和白介素-10（Interleukin-10，IL-10）的濃度。計算肺組織含水量濕重/干重比(Wet/Dry ratio W/D)；觀察比較各組動物肺組織及氣管標(biāo)本大體改變、HE染色常規(guī)病理學(xué)改變。 2、48只健康Wistar大鼠同樣按照上述方法制作熱煙吸入性損傷動物模型，隨機數(shù)字表法分為熱煙霧吸入致傷組(24只)和經(jīng)尾靜脈移植hMSCs治療組（24只），各組再按照致傷后時間點分為3個亞組（2h、4h、8h）。熱煙霧吸入致傷組和經(jīng)尾靜脈移植hMSCs治療組分別于致傷后2h,4h,8h活殺。測定血清及支氣管肺泡灌洗液（BALF）中腫瘤壞死因子-α（TNF-α）濃度；白介素-6（,IL-6）和白介素-10（IL-10）的含量。計算肺組織含水量濕重/干重比(W/D)；觀察比較各組動物肺組織及氣管標(biāo)本大體改變、HE染色常規(guī)病理學(xué)改變。 結(jié)果 1、與正常對照組（C組）比較，熱煙霧吸入性肺損傷組（SQ組）各時間點血清促炎、抗炎因子均顯著升高；各時間點支氣管肺泡灌洗液促炎因子顯著升高，抗炎因子無明顯變化。與SQ組比較，經(jīng)氣管移植hMSCs治療組（MQ組）在4h、8h血清促炎因子顯著下降，抗炎因子顯著升高，在2h時炎性因子無明顯變化。各時間點肺泡灌洗液促炎因子顯著降低，而抗炎因子僅在4h、8h顯著升高。與C組比較，SQ組和MQ組肺水質(zhì)量分數(shù)均明顯升高，MQ組肺水質(zhì)量分數(shù)較SQ組顯著降低；大體觀察和組織病理學(xué)顯示：MQ組肺組織損傷程度較SQ組明顯改善； 2、與正常對照組（C組）比較，熱煙霧吸入性肺損傷組（SV組）各時間點血清促炎、抗炎因子均顯著升高；各時間點肺泡灌洗液促炎因子顯著升高，，抗炎因子無明顯變化。與SV組比較，經(jīng)尾靜脈注入hMSCs治療組（MV組）各時間點血清促炎因子顯著下降，抗炎因子顯著升高。各時間點肺泡灌洗液促炎因子顯著降低，而抗炎因子僅在4h、8h顯著升高。與C組比較，SV組和MV組肺水質(zhì)量分數(shù)均明顯升高，MV組肺水質(zhì)量分數(shù)較SV組顯著降低；大體觀察和組織病理學(xué)顯示：MV組肺組織損傷程度較SV組明顯改善。 結(jié)論 1、經(jīng)氣管移植hMSCs后對熱煙霧導(dǎo)致的急性肺損傷有一定的保護作用。 2、經(jīng)尾靜脈注入hMSCs能降低熱煙霧吸入性肺損傷早期促炎因子水平，升高抗炎因子水平，減輕全身炎癥反應(yīng)，對損傷肺組織具有一定的保護作用。 3、干細胞經(jīng)這兩種移植途徑均可對損傷肺組織發(fā)揮保護作用。
[Abstract]:objective
1, the effect of bone marrow mesenchymal stem cells (MSCs) on heat smoke inhalation induced lung injury in rats was investigated.
2, the effect of human bone marrow mesenchymal stem cells injected into the tail vein on hot smoke inhalation lung injury in rats was investigated.
3, the effect of different transplantation approaches on stem cells was studied.
Method
1, developed hot smoke generating device, simulating fire scene hot smoke environment. 56 healthy male Wistar rats were randomly divided into normal control group (8 rats), injury group (24 rats) inhalation of hot smoke and trachea transplantation hMSCs treatment group (24), after the two groups according to the time points after injury were divided into 3 subgroups (2h, 4h, 8h). The control group after 8h killed, hot smoke inhalation injury group and hMSCs treatment group were transplanted trachea after injury in 2H, 4h, 8h were killed. The serum and bronchoalveolar lavage fluid (BronchoalveolarLavage Fluid BALF) in tumor necrosis factor alpha (Tumor alpha TNF- alpha Necrosis Factor-), interleukin -6 (Interleukin-6, IL-6) and interleukin -10 (Interleukin-10, IL-10). The concentration of calculating the water content of lung tissue wet weight / dry weight ratio (Wet/Dry ratio W/D); observed in lung tissue of animal and gas samples general HE staining pathological change Learn to change.
2,48 healthy Wistar rats were also produced in accordance with the above method of hot smoke inhalation injury animal model, randomly divided into hot smoke inhalation injury group (24 rats) and intravenous transplantation of hMSCs treatment group (24 rats), each group according to the time point after injury were divided into 3 subgroups (2h, 4h, 8h). The injury group and intravenous transplantation of hMSCs treatment group respectively at 2h after injury, inhalation of hot smoke 4H killed 8h. Determination of serum and bronchoalveolar lavage fluid (BALF) in tumor necrosis factor alpha (TNF- alpha) concentration; interleukin (-6, IL-6) and white interleukin -10 (IL-10). The contents of calculating the water content of lung tissue wet weight / dry weight ratio (W/D); observed lung and trachea animal specimens in general change, HE staining pathological changes.
Result
1, with the normal control group (group C), hot smoke inhalation injury group (SQ group) each time point serum proinflammatory and anti-inflammatory cytokines were significantly increased at each time point; bronchoalveolar lavage inflammatory cytokines increased significantly, anti-inflammatory factor has no obvious change. Compared with SQ group, trachea transplantation hMSCs the treatment group (MQ group) in 4h, 8h of serum proinflammatory cytokines decreased significantly, anti-inflammatory factor increased significantly in 2H and inflammatory cytokines had no obvious change. At each time point in bronchoalveolar lavage fluid of pro-inflammatory cytokines and anti-inflammatory cytokines decreased significantly only in 4h, 8h was significantly increased. Compared with C group, SQ group and MQ group of lung water content were significantly increased in MQ group, lung water mass fraction was significantly lower than that of SQ group; morphology and histopathology showed that the degree of lung injury in group MQ was significantly better than those in SQ group;
2, with the normal control group (group C), hot smoke inhalation injury group (SV group) each time point serum proinflammatory and anti-inflammatory cytokines were significantly increased at each time point; bronchoalveolar lavage fluid of proinflammatory cytokines increased significantly, anti-inflammatory factor has no obvious change. Compared with SV group, intravenous injection hMSCs treatment group (group MV) at different time points of serum proinflammatory cytokines decreased significantly, anti-inflammatory factor significantly increased. At each time point in bronchoalveolar lavage fluid of pro-inflammatory cytokines and anti-inflammatory cytokines decreased significantly only in 4h, 8h was significantly increased. Compared with C group, SV group and MV group of lung water content were significantly increased, MV group of lung water content was significantly lower than that of SV group; morphology and histopathology showed: in MV group the degree of lung injury was significantly better than those in SV group.
conclusion
1, lead to acute lung heat smoke trachea transplantation hMSCs after injury has a protective effect.
2, injection of hMSCs through the caudal vein can reduce the level of proinflammatory factors in early stage, increase the level of anti-inflammatory factors, relieve systemic inflammatory response and protect lung tissue.
3, the stem cells can play a protective role in the injury of lung tissue through these two kinds of transplantation.

【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R563.8

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