本文關鍵詞： 雌二醇 哮喘 引喘潛伏期 氣道炎癥　出處：《細胞與分子免疫學雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的觀察雌二醇對哮喘小鼠引喘潛伏期及氣道炎癥的影響。方法雄性BALB/c小鼠隨機分為正常對照組、卵清蛋白(OVA)誘發(fā)哮喘組、400μg/kg雌二醇處理組、400μg/kg雌二醇聯(lián)合7 mg/kg他莫昔芬組,每組10只。OVA誘發(fā)哮喘組、雌二醇處理組、雌二醇聯(lián)合他莫昔芬組通過OVA致敏、激發(fā)建立哮喘模型,正常對照組以生理鹽水替代。雌二醇處理組、雌二醇聯(lián)合他莫昔芬組于每次激發(fā)前4 h給予400μg/kg雌二醇腹腔注射,雌二醇聯(lián)合他莫昔芬組在注射雌二醇前30 min給予7 mg/kg他莫昔芬腹腔注射。正常對照組、OVA誘發(fā)哮喘組腹腔注射生理鹽水作對照。于末次激發(fā)后24 h,測定各組小鼠引喘潛伏期。收集支氣管肺泡灌洗液(BALF)檢測白細胞總數(shù)并進行嗜酸性粒細胞和淋巴細胞分類計數(shù),ELISA檢測BALF中白細胞介素4(IL-4)、IL-13水平。HE染色觀察肺組織病理變化。結果與正常對照組比較,OVA誘發(fā)哮喘組、雌二醇處理組、雌二醇聯(lián)合他莫昔芬組引喘潛伏期均縮短;雌二醇處理組較OVA誘發(fā)哮喘組、雌二醇聯(lián)合他莫昔芬組引喘潛伏期縮短;OVA誘發(fā)哮喘組與雌二醇聯(lián)合他莫昔芬組引喘潛伏期無顯著性差異。與正常對照組比較,OVA誘發(fā)哮喘組、雌二醇處理組、雌二醇聯(lián)合他莫昔芬組IL-4、IL-13水平升高;雌二醇處理組較OVA誘發(fā)哮喘組、雌二醇聯(lián)合他莫昔芬組IL-4、IL-13水平升高;OVA誘發(fā)哮喘組與雌二醇聯(lián)合他莫昔芬組IL-4、IL-13水平相當。與正常對照組比較,OVA誘發(fā)哮喘組、雌二醇處理組、雌二醇聯(lián)合他莫昔芬組BALF白細胞總數(shù)及嗜酸性粒細胞、淋巴細胞分類計數(shù)增加;雌二醇處理組較OVA誘發(fā)哮喘組、雌二醇聯(lián)合他莫昔芬組白細胞總數(shù)及嗜酸性粒細胞、淋巴細胞分類計數(shù)增加;OVA誘發(fā)哮喘組與雌二醇聯(lián)合他莫昔芬組比較,白細胞總數(shù)及嗜酸性粒細胞、淋巴細胞分類計數(shù)無顯著性差異。正常對照組小鼠氣道黏膜無充血腫脹,氣道周圍無炎性細胞浸潤;OVA誘發(fā)哮喘組、雌二醇聯(lián)合他莫昔芬組小鼠氣道黏膜充血水腫,支氣管壁及周圍有較多炎細胞浸潤;雌二醇處理組較OVA誘發(fā)哮喘組、雌二醇聯(lián)合他莫昔芬組上述改變更為嚴重。結論雌二醇能縮短哮喘小鼠引喘潛伏期,促進氣道炎癥細胞浸潤及IL-4、IL-13的表達。
[Abstract]:Objective to observe the effects of estradiol on asthmatic latency and airway inflammation in asthmatic mice. Methods male BALB/c mice were randomly divided into control group and ovalbumin ovalbumin induced asthma group (400 渭 g / kg estradiol plus 7 mg/kg tamoxifen). 10 rats in each group were sensitized with estradiol, estradiol and tamoxifen by OVA, and the normal control group was replaced by normal saline. The estradiol combined with tamoxifen group was injected intraperitoneally with 400 渭 g / kg estradiol 4 hours before each stimulation. The estradiol combined with tamoxifen group was intraperitoneally injected with tamoxifen for 7 mg/kg at 30 min before estradiol injection. The asthmatic group induced by normal control group was treated by intraperitoneal injection of normal saline 30 min before injection of estradiol. 24 hours after the last challenge, the dose of tamoxifen in each group was measured. Bronchoalveolar lavage fluid (BALF) was collected to detect the total number of leukocytes and eosinophilic granulocytes and lymphocytes. Elisa was used to detect IL-13 level in BALF. The pathological changes of lung tissue were observed by HE staining. Normal control group compared with OVA induced asthma group, In estradiol group, estradiol combined with tamoxifen group, the incubation period of asthma induced by estradiol and tamoxifen was shortened, and the asthma induced by estradiol group was higher than that in OVA group. There was no significant difference between estradiol combined with tamoxifen group and estradiol combined with tamoxifen group. The level of IL-4 and IL-13 in estradiol combined with tamoxifen group was higher than that in OVA induced asthma group. The levels of IL-4 and IL-13 in the estradiol combined with tamoxifen group were similar to those in the OVA induced asthma group and the estradiol combined tamoxifen group, and were compared with those in the OVA induced asthma group and the estradiol treated group. The total number of BALF leukocytes and eosinophils and the number of eosinophilic granulocytes in the estradiol combined with tamoxifen group were higher than those in the OVA induced asthma group and the estradiol combined with tamoxifen group. Compared with estradiol combined with tamoxifen group, there was no significant difference in total leukocyte count, eosinophilic granulocyte and lymphocyte classification count between OVA induced asthma group and OVA induced asthma group, but there was no hyperemia and swelling in airway mucosa of normal control group. There were no inflammatory cell infiltration around the airway. OVA-induced asthma group, estradiol combined with tamoxifen group mice airway mucosal congestion and edema, bronchial wall and surrounding inflammatory cell infiltration, estradiol treatment group than OVA induced asthma group, Conclusion estradiol can shorten the latent period of asthma induced asthma and promote the infiltration of airway inflammatory cells and the expression of IL-4 IL-13 in asthmatic mice.
【作者單位】： 徐州醫(yī)科大學附屬醫(yī)院呼吸內科;
【分類號】：R562.25
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本文編號：1512516