本文關鍵詞： 結(jié)核分枝桿菌 HIV/AIDS 結(jié)核抗原 ELISPOT　出處：《浙江中醫(yī)藥大學》2012年碩士論文　論文類型：學位論文

【摘要】：目的：探討酶聯(lián)免疫斑點試驗聯(lián)合結(jié)核抗原檢測方法對AIDS合并結(jié)核患者的臨床價值。 方法：32例AIDS患者分成結(jié)核組和非結(jié)核組,對兩組患者進行ELISPOT.結(jié)核抗原、血培養(yǎng)、痰涂片、PPD、結(jié)核抗體檢測,分析ELISPOT聯(lián)合結(jié)核抗原檢測方法是否較其他結(jié)核檢測方法更有優(yōu)勢。 結(jié)果：結(jié)核組各種檢測方法的陽性率分別為：血培養(yǎng)：7.1%、痰涂片：7.1%、PPD：14.3%、結(jié)核抗體：21.4%、結(jié)核抗原：28.6%、ELISPOT:85.7%、ELISPOT聯(lián)合結(jié)核抗原檢測(簡稱聯(lián)合檢測)：92.9%,聯(lián)合檢測與血培養(yǎng)、痰涂片、PPD、結(jié)核抗體、結(jié)核抗原檢測方法比較均有統(tǒng)計學意義(P0.005),與ELISPOT檢測法比較無統(tǒng)計學意義(P0.9)。非結(jié)核組各種檢測方法的陽性率分別為：血培養(yǎng)：0、痰涂片：0、PPD：0、結(jié)核抗體：16.7%、結(jié)核抗原：11.1%、ELISPOT:0.聯(lián)合檢測法：11.1%。結(jié)核組與非結(jié)核組各種檢測方法比較,僅有ELISPOT及聯(lián)合檢測方法有統(tǒng)計學意義(P0.0001),其他檢測方法無統(tǒng)計學意義。 結(jié)論： 第一：AIDS合并結(jié)核感染患者,ELISPOT聯(lián)合結(jié)核抗原檢測或單獨ELISPOT檢測具有更高的陽性率和敏感性。AIDS患者懷疑結(jié)核感染無法確診時,可應用ELISPOT聯(lián)合結(jié)核抗原檢測方法,避免臨床漏診,聯(lián)合方法值得在臨床推廣 第二：傳統(tǒng)方法檢測(血培養(yǎng)、痰涂片、PPD、結(jié)核抗體、結(jié)核抗原)在結(jié)核及非結(jié)核組無統(tǒng)計學差異,傳統(tǒng)方法單獨檢測在HIV人群中診斷結(jié)核效果不顯著。
[Abstract]:Objective: to investigate the clinical value of Elisa combined with tuberculosis antigen detection in AIDS patients with tuberculosis. Methods 32 patients with AIDS were divided into tuberculosis group and non-tuberculosis group. The two groups were treated with ElisPOT.TB antigen, blood culture, sputum smear, tuberculosis antibody detection, and whether ELISPOT combined with TB antigen detection method was superior to other TB detection methods. Results: the positive rates of blood culture, sputum smear and sputum smear were as follows: blood culture, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, tuberculosis antibody, tuberculosis antigen: 1. 28. 628. 8. 7. 7. 7. 8. 7. 7. 7. 7. The positive rates were: blood culture, sputum smear, tuberculosis antibody, sputum smear, sputum smear, tuberculosis antibody. The positive rates of various methods in non-tuberculosis group were: blood culture 0, sputum smear 0: 0, tuberculosis antibody: 16.7g, tuberculosis antigen 11.1%, ELISPOT: 0. The positive rates of each method were as follows: blood culture: 0, sputum smear: 0, tuberculosis antibody: 16.7g, tuberculosis antigen 11.1a, ELISPOT: 0.The positive rates of the methods were as follows: blood culture: 0, sputum smear: 0, TB antibody: 16.7. Test method: 11.1. comparison of various methods of detection between tuberculosis group and non-tuberculosis group, Only ELISPOT and combined detection methods had statistical significance (P 0.0001), while other methods had no statistical significance. Conclusion:. No. 1: AIDS patients with tuberculosis infection may use ELISPOT combined with tuberculosis antigen detection method to avoid clinical misdiagnosis if the positive rate and sensitivity of either Elispot combined with tuberculosis antigen detection or ELISPOT alone test are higher and higher. When AIDS patients suspected that tuberculosis infection could not be diagnosed, the method of ELISPOT combined with tuberculosis antigen detection can be used to avoid clinical misdiagnosis. Combined method is worth popularizing in clinic. Second, the traditional methods (blood culture, sputum smear, tuberculosis antibody, tuberculosis antigen) had no statistical difference between tuberculosis and non-tuberculosis group, but the traditional method alone in the diagnosis of tuberculosis in HIV population did not have significant effect.
【學位授予單位】：浙江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R446.6;R512.91;R521

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