超聲心動圖觀察慢性間歇性低氧新西蘭兔左心結(jié)構(gòu)和功能的實(shí)驗(yàn)研究
發(fā)布時間：2019-02-23 15:31
【摘要】：目的：建立慢性間歇性低氧（CIH）新西蘭兔模型，在二維超聲及M型超聲心動圖技術(shù)觀察左心常規(guī)結(jié)構(gòu)和功能的基礎(chǔ)上，進(jìn)一步應(yīng)用頻譜多普勒、組織多普勒成像等彩色多普勒超聲技術(shù)來觀測CIH新西蘭大白兔早期血流動力學(xué)和左心收縮、舒張功能及結(jié)構(gòu)動態(tài)變化過程。 方法：將新西蘭兔置于CIH艙內(nèi)，通過氣體控制系統(tǒng)向CIH艙內(nèi)循環(huán)通入氮?dú)夂脱鯕庖阅M慢性間歇性低氧環(huán)境，1次循環(huán)時間為170s，使艙內(nèi)氧濃度波動于8～21%，即充入氮?dú)馐笴IH艙內(nèi)氧濃度由21%下降至8～12%，約需80s，維持在最低氧濃度，持續(xù)約25s；隨后充入氧氣升高CIH艙內(nèi)氧濃度，使CIH艙內(nèi)氧濃度恢復(fù)到21%，約需35s，并維持約25s，至下一循環(huán)。每天持續(xù)6小時（h），6天/周（d/w），共8周（w）。應(yīng)用GE-Vivid7型彩色超聲診斷儀于造模第0、1、2、4、6、8w進(jìn)行超聲心動圖檢查。心肌及主動脈標(biāo)本送病理學(xué)檢查，HE染色觀察形態(tài)學(xué)變化。 結(jié)果：①左室結(jié)構(gòu)變化：與0w比較，1w、2w、4w時各參數(shù)無統(tǒng)計學(xué)意義（P＞0.05）,6w、8w時LVPW、IVS增厚，差異有統(tǒng)計學(xué)意義（P＜0.05），8w時LVEDd、LV（左右徑）、LA（前后徑）、LA（左右徑）增大，AO增寬，差異有統(tǒng)計學(xué)意義（P＜0.05）。②左室收縮功能參數(shù)：EF（Simpson法）于2w和4w升高（P＜0.05），6w、8w逐漸減低，，但與0周比無統(tǒng)計學(xué)意義（P＞0.05），IVCT于1w、2w和4w時縮短（P＜0.05），ET于4w縮短（P＜0.05），IVS-Sm于2w、4w、6w增高（P＜0.05）。③左室舒張功能參數(shù)：與0w比較， MV E峰、IVS-Em于2w、4w升高，8w減低，IVS-Am于8w增高，IVS-Em/Am于4w升高，8w減低（P＜0.05），IVRT于1w、2w、4w縮短，8w時延長（P＜0.05），DT于8w延長，Vp于8w減低（P＜0.05），Adur在各時間點(diǎn)差別無統(tǒng)計學(xué)意義（P＞0.05）。④左室心肌病理改變：0w時心肌細(xì)胞完整，排列整齊，無血管擴(kuò)張及血管壁增厚等表現(xiàn)；2w、4w時鏡下可見心肌纖維排列略顯紊亂，心肌細(xì)胞輕度腫脹，細(xì)胞核體積輕度增大，間隙略增寬，細(xì)胞間質(zhì)輕度水腫；6w，8w時鏡下可見心肌細(xì)胞肥大，細(xì)胞核增大，心肌纖維增粗且排列紊亂、間隙增寬，部分細(xì)胞可見空泡變性，胞漿溶解，細(xì)胞間質(zhì)充血水腫。 結(jié)論：①IVCT和IVRT是反映CIH新西蘭兔左室收縮和舒張功能代償?shù)拿舾行灾笜?biāo)。②左心對CIH的應(yīng)答呈時間依賴性，先發(fā)生了獨(dú)立于左室壁增厚的收縮舒張功能代償，之后左室功能代償依賴于左室結(jié)構(gòu)的改變。③左室舒張功能受損早于收縮功能。
[Abstract]:Objective: to establish a new Zealand rabbit model of chronic intermittent hypoxic (CIH) and to observe the conventional structure and function of the left heart by two dimensional echocardiography and M-mode echocardiography. Tissue Doppler imaging and other color Doppler ultrasound techniques were used to observe the dynamic changes of hemodynamics and left ventricular systolic diastolic function and structure in CIH New Zealand rabbits. Methods: the New Zealand rabbits were placed in the CIH cabin, and the nitrogen and oxygen were circulated through the gas control system to simulate the chronic intermittent hypoxic environment. The cycle time was 170 s, and the oxygen concentration in the chamber fluctuated at 821 h. The oxygen concentration in the CIH chamber was decreased from 21% to 812 seconds, which was maintained at the lowest oxygen concentration for about 25 s. Then the oxygen is filled to increase the oxygen concentration in the CIH chamber, so that the oxygen concentration in the CIH chamber recovers to 21. It takes about 35 seconds and maintains about 25 seconds to the next cycle. Daily duration of 6 hours (h), 6 days / week (d / w), a total of 8 weeks (w). Echocardiography was performed by using GE-Vivid7 color ultrasound diagnostic instrument at the 0th week of model making. The myocardial and aortic specimens were examined by pathology and the morphological changes were observed by HE staining. Results: 1the changes of left ventricular structure: there was no significant difference in the parameters of the left ventricular structure between the two groups (P > 0. 05), but there was no significant difference in the thickness of LVPW,IVS at 6 and 8 weeks (P < 0. 05), and the LVEDd,LV (left and right diameter) at 8 weeks. LA (anteroposterior diameter), LA (left and right diameter) increased and AO widened (P < 0. 05). The left ventricular systolic function parameter (: EF (Simpson method) increased at 2 and 4 weeks (P < 0. 05), and gradually decreased at 6 and 8 weeks. But there was no significant difference between 0 and 0 weeks (P > 0. 05), IVCT at 1 and 4 weeks) (P < 0. 05), ET at 4 weeks), IVS-Sm at 2 ws and 4 ws. 3 left ventricular diastolic function parameters: compared with 0 w, IVS-Em increased at 4 weeks, decreased at 8 weeks, IVS-Am increased at 8 weeks, IVS-Em/Am increased at 4 weeks and decreased at 8 weeks (P < 0. 05), IVRT at 1 week, P < 0. 05), and left ventricular diastolic function increased at 6 weeks (P < 0. 05). At 2 weeks, 4 weeks, 8 weeks later (P < 0. 05), Vp decreased at 8 weeks (P < 0. 05), but decreased at 8 weeks (P < 0. 05), and decreased at 8 weeks (P < 0. 05). There was no significant difference in Adur at different time points (P > 0. 05). 4 the pathological changes of left ventricular myocardium were as follows: myocardial cells were intact and arranged at 0 w, without vasodilation and wall thickening. At 2 weeks after 4 weeks, the arrangement of myocardial fibers was slightly disordered, the myocardial cells were slightly swollen, the nuclear volume was slightly enlarged, the gap was slightly widened, and the interstitial cells were slightly edema. At 6 weeks or 8 weeks, myocytes were hypertrophy, nucleus was enlarged, myocardial fibers were thicker and disarranged, gap widened, vacuolar degeneration was observed in some cells, cytoplasm was dissolved, and interstitial hyperemia and edema were observed. Conclusion: 1IVCT and IVRT are sensitive indexes to reflect left ventricular systolic and diastolic function compensation in CIH New Zealand rabbits. 2 the response of left ventricular to CIH is time-dependent, and the systolic and diastolic function compensations are independent of left ventricular wall thickening. After that, left ventricular function compensation depended on the change of left ventricular structure. 3 left ventricular diastolic function was damaged earlier than systolic function.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R540.45
本文編號：2428957
[Abstract]:Objective: to establish a new Zealand rabbit model of chronic intermittent hypoxic (CIH) and to observe the conventional structure and function of the left heart by two dimensional echocardiography and M-mode echocardiography. Tissue Doppler imaging and other color Doppler ultrasound techniques were used to observe the dynamic changes of hemodynamics and left ventricular systolic diastolic function and structure in CIH New Zealand rabbits. Methods: the New Zealand rabbits were placed in the CIH cabin, and the nitrogen and oxygen were circulated through the gas control system to simulate the chronic intermittent hypoxic environment. The cycle time was 170 s, and the oxygen concentration in the chamber fluctuated at 821 h. The oxygen concentration in the CIH chamber was decreased from 21% to 812 seconds, which was maintained at the lowest oxygen concentration for about 25 s. Then the oxygen is filled to increase the oxygen concentration in the CIH chamber, so that the oxygen concentration in the CIH chamber recovers to 21. It takes about 35 seconds and maintains about 25 seconds to the next cycle. Daily duration of 6 hours (h), 6 days / week (d / w), a total of 8 weeks (w). Echocardiography was performed by using GE-Vivid7 color ultrasound diagnostic instrument at the 0th week of model making. The myocardial and aortic specimens were examined by pathology and the morphological changes were observed by HE staining. Results: 1the changes of left ventricular structure: there was no significant difference in the parameters of the left ventricular structure between the two groups (P > 0. 05), but there was no significant difference in the thickness of LVPW,IVS at 6 and 8 weeks (P < 0. 05), and the LVEDd,LV (left and right diameter) at 8 weeks. LA (anteroposterior diameter), LA (left and right diameter) increased and AO widened (P < 0. 05). The left ventricular systolic function parameter (: EF (Simpson method) increased at 2 and 4 weeks (P < 0. 05), and gradually decreased at 6 and 8 weeks. But there was no significant difference between 0 and 0 weeks (P > 0. 05), IVCT at 1 and 4 weeks) (P < 0. 05), ET at 4 weeks), IVS-Sm at 2 ws and 4 ws. 3 left ventricular diastolic function parameters: compared with 0 w, IVS-Em increased at 4 weeks, decreased at 8 weeks, IVS-Am increased at 8 weeks, IVS-Em/Am increased at 4 weeks and decreased at 8 weeks (P < 0. 05), IVRT at 1 week, P < 0. 05), and left ventricular diastolic function increased at 6 weeks (P < 0. 05). At 2 weeks, 4 weeks, 8 weeks later (P < 0. 05), Vp decreased at 8 weeks (P < 0. 05), but decreased at 8 weeks (P < 0. 05), and decreased at 8 weeks (P < 0. 05). There was no significant difference in Adur at different time points (P > 0. 05). 4 the pathological changes of left ventricular myocardium were as follows: myocardial cells were intact and arranged at 0 w, without vasodilation and wall thickening. At 2 weeks after 4 weeks, the arrangement of myocardial fibers was slightly disordered, the myocardial cells were slightly swollen, the nuclear volume was slightly enlarged, the gap was slightly widened, and the interstitial cells were slightly edema. At 6 weeks or 8 weeks, myocytes were hypertrophy, nucleus was enlarged, myocardial fibers were thicker and disarranged, gap widened, vacuolar degeneration was observed in some cells, cytoplasm was dissolved, and interstitial hyperemia and edema were observed. Conclusion: 1IVCT and IVRT are sensitive indexes to reflect left ventricular systolic and diastolic function compensation in CIH New Zealand rabbits. 2 the response of left ventricular to CIH is time-dependent, and the systolic and diastolic function compensations are independent of left ventricular wall thickening. After that, left ventricular function compensation depended on the change of left ventricular structure. 3 left ventricular diastolic function was damaged earlier than systolic function.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R540.45
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