本文選題：腦 + 分子探針技術(shù)　； 參考：《北京大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年02期

【摘要】：目的:對比光、磁兩種探針示蹤顯示腦組織間隙(interstitial space,ISS)內(nèi)分子擴散與團流運動的結(jié)果,研究腦組織液(interstitial fluid,ISF)在ISS內(nèi)的分區(qū)引流特征。方法:36只SD大鼠隨機分為熒光檢查組(18只)和磁示蹤組(18只),每組再隨機分為尾狀核(caudate nucleus,Cn)、丘腦(thalamus,T)和黑質(zhì)(substantia nigra,Sn)3個亞組,每亞組6只。熒光檢查組:參考腦立體定位圖譜,選取冠狀位蒼白球為中心層面,以Cn、T和Sn為靶點進行穿刺定位,分別導(dǎo)入2μL 10 mmol/L熒光黃(lucifer yellow,LY)于相應(yīng)腦區(qū)的中心位置,于Cn 3 h、T 2 h和Sn 1 h對大鼠用4%(體積分數(shù))多聚甲醛進行心臟灌注固定后,將取出的鼠腦置于腦切片模具中,沿視交叉向后切片,以進針位點所在的冠狀切片為中心層面,計為1片,前取3片,后取2片,每片厚約1 mm,共6片待檢測,應(yīng)用激光掃描共聚焦顯微鏡(laser scanning confocal microscope,LSCM)對離體切片進行采集,測量離體切片LY擴散的面積;磁示蹤組大鼠也以Cn、T和Sn為靶點進行穿刺定位導(dǎo)入2μL 10 mmol/L釓噴酸葡胺(gadolinium-diethylene triamine pentaacetic acidm,Gd-DTPA),應(yīng)用磁共振(magnetic resonance imaging,MRI)動態(tài)監(jiān)測示蹤劑在大鼠腦內(nèi)的擴散,并利用Radiant軟件測量Gd-DTPA的擴散面積。結(jié)果:LY與Gd-DTPA在Cn、T和Sn的ISS內(nèi)擴散區(qū)域各不相同,Cn亞組LY與Gd-DTPA導(dǎo)入3 h后,比較1~6層LY與Gd-DTPA擴散面積為:(10.95±4.27)mm~2vs.(8.33±2.25)mm~2、(18.16±4.74)mm~2vs.(16.42±2.88)mm~2、(24.57±3.65)mm~2vs.(20.75±2.29)mm~2、(34.81±3.32)mm~2vs.(28.88±1.51)mm~2、(30.53±3.12)mm~2vs.(20.92±2.75)mm~2、(12.15±4.92)mm~2vs.(10.00±1.89)mm~2,對其在每層兩組間擴散面積進行t檢驗,擴散面積差異均無統(tǒng)計學(xué)意義(t=0.940,P=0.400;t=0.546,P=0.614;t=1.534,P=0.200;t=2.809,P=0.480;t=2.693,P=0.055;t=0.707,P=0.518);T亞組中LY與Gd-DTPA導(dǎo)入2 h后,比較1~6層LY與Gd-DTPA擴散面積為:(5.56±4.61)mm~2vs.(3.33±2.25)mm~2、(16.21±3.36)mm~2vs.(11.42±2.88)mm~2、(19.00±5.21)mm~2vs.(15.75±2.29)mm~2、(25.32±5.49)mm~2vs.(22.33±3.25)mm~2、(17.34±5.31)mm~2vs.(15.92±2.75)mm~2、(7.67±6.19)mm~2vs.(5.00±1.89)mm~2,對其在每層兩組間擴散面積進行t檢驗,差異均無統(tǒng)計學(xué)意義(t=0.753,P=0.493;t=1.875,P=0.134;t=0.990,P=0.378;t=0.810,P=0.464;t=0.413,P=0.701;t=0.716,P=0.514);Sn亞組LY與Gd-DTPA導(dǎo)入1 h后,比較1~6層LY與Gd-DTPA擴散面積為:(6.78±4.56)mm~2vs.(4.75±2.00)mm~2、(12.65±5.04)mm~2vs.(10.44±1.13)mm~2、(19.51±6.54)mm~2vs.(17.55±0.30)mm~2、(28.72±5.45)mm~2vs.(24.48±1.32)mm~2、(21.34±4.42)mm~2vs.(17.72±0.25)mm~2、(13.00±5.46)mm~2vs.(12.00±2.88)mm~2,對其在每層間擴散面積進行t檢驗,擴散面面積的差異均無統(tǒng)計學(xué)意義(t=0.705,P=0.519;t=0.743,P=0.499;t=0.517,P=0.656;t=1.310,P=0.260;t=1.416,P=0.292;t=0.281,P=0.793),但LY擴散面積略大于Gd-DTPA。結(jié)論:熒光法證實了磁示蹤法發(fā)現(xiàn)的ISF在ISS內(nèi)的分區(qū)引流特征,并且可為磁示蹤法提供離體驗證的技術(shù)與方法。熒光ISS成像法具有更高的對比度和分辨率,得到了更加精細的ISF引流分區(qū)區(qū)域。
[Abstract]:Aim: to compare the results of molecular diffusion and mass flow motion in interstitial space ISSs (interstitial space ISSs) with two magnetic probes, and to study the regional drainage characteristics of interstitial fluid flow (ISF) in ISS. Methods Thirty six Sprague-Dawley rats were randomly divided into three subgroups: fluorescent inspection group (n = 18) and magnetic tracer group (n = 18). Each group was further divided into three subgroups: caudate nucleusus caudate, thalamus and substantia nigragragragrausin, with 6 rats in each subgroup. Fluorescence inspection group: referring to the stereotaxic map of the brain, the coronal globus pallidus was selected as the central layer, the CNT and Sn were used as the target for puncture localization, and 2 渭 L 10 mmol/L fluorescent lucifer low y) was introduced into the central position of the corresponding brain region, respectively. The rats were fixed with 4% (volume fraction) of paraformaldehyde for 2 h and Sn 1 h respectively. The brain was placed in the brain slice mould and sliced backward along the optic chiasma. The coronal section of the needle was taken as the central layer. There were 1 slice, 3 pieces before and 2 pieces after, each slice was about 1 mm thick, 6 pieces were to be detected. Laser scanning confocal microscope (LSCM) was used to collect the in vitro slice and the area of LY diffusion was measured. In the magnetic tracer group, CNT and Sn were also used as the target point for the puncture localization of 2 渭 L 10 mmol/L Gadolinium-diethylene triamine pentaacetic acidmite Gd-DTPAA. The diffusion of the tracer in the rat brain was dynamically monitored by magnetic resonance imaging technique, and the diffusion area of Gd-DTPA was measured by Radiant software. 緇撴灉:LY涓嶨d-DTPA鍦–n,T鍜孲n鐨処SS鍐呮墿鏁ｅ尯鍩熷悇涓嶇浉鍚，

本文編號：1964718