本文選題：磁共振成像　切入點：淋巴成像　出處：《放射學(xué)實踐》2015年12期

【摘要】：目的:探討對BALB/c小鼠乳腺采用Gd-DTPA行間質(zhì)性磁共振淋巴成像(MRL)的檢查技術(shù)并評價其可行性;比較BALB/c小鼠和昆明小鼠乳腺間質(zhì)性MRL的差異。方法:在3只BALB/c小鼠右側(cè)第5組乳腺鄰近皮內(nèi)注射10μL(1.0μmol)Gd-DTPA溶液,用同樣方法分別于5只BALB/c小鼠和5只昆明小鼠(雌性,大小約10周)右側(cè)第5組乳腺鄰近皮內(nèi)注射相同劑量Gd-DTPA溶液。每只實驗動物均在注射對比劑前及對比劑注射后40s、2min 10s、5min10s、8min 10s、11min 10s、14min 10s、17min 10s、20min 10s行乳腺MRL掃描,測量增強(qiáng)后淋巴結(jié)的信號強(qiáng)度并計算淋巴結(jié)-肌肉信號強(qiáng)度比(SIR)。繪制時間-信號強(qiáng)度比曲線,比較兩種小鼠淋巴結(jié)增強(qiáng)效果并進(jìn)行統(tǒng)計學(xué)分析。結(jié)果:注射Gd-DTPA后40s,可見BALB/c小鼠右側(cè)腹股溝淋巴結(jié)明顯強(qiáng)化;時間-信號強(qiáng)度比曲線在注射對比劑后40s內(nèi)快速上升,隨后快速下降,20min時恢復(fù)至平掃時的信號強(qiáng)度;對比劑注射前后各時間點BALB/c小鼠與昆明小鼠右側(cè)腹股溝淋巴結(jié)SIR的比較采用F檢驗,差異均無統(tǒng)計學(xué)意義(P0.05)。所有實驗小鼠皮內(nèi)注射Gd-DTPA后未見明顯不良反應(yīng)。結(jié)論:1利用Gd-DTPA進(jìn)行小鼠乳腺間質(zhì)性MR淋巴成像是一種安全、重復(fù)性好的可行性檢查技術(shù);2昆明小鼠可代替BALB/c小鼠進(jìn)行乳腺間質(zhì)MRL研究。
[Abstract]:Objective: to evaluate the feasibility of Gd-DTPA interstitial magnetic resonance lymphography (MRL) in mammary glands of BALB/c mice, and to compare the difference between BALB/c mice and Kunming mice.Methods: 10 渭 L 1. 0 渭 mol)Gd-DTPA solution was injected into the right mammary adjacent skin of 3 BALB/c mice in the right fifth group. The same dose of Gd-DTPA solution was injected into 5 BALB/c mice and 5 Kunming mice (female, about 10 weeks in size) in the right fifth group.Mammary MRL scanning was performed on each animal before and after injection of contrast agent 40 s / 2 min 10 s / 5 min 10 min 10 s / 8 min 10 s / 11 min 10 s / 14 min 10 s / 14 min 10 s / 10 s / 10 min 10 s / 10 s. The signal intensity of enhanced lymph nodes was measured and the signal intensity ratio of lymph node to muscle was calculated.Time-signal intensity ratio curve was drawn to compare the enhancement effect of two kinds of mice lymph nodes and to make statistical analysis.Results: 40 s after Gd-DTPA injection, the right inguinal lymph nodes of BALB/c mice were obviously enhanced, the time-signal intensity ratio curve increased rapidly within 40 s after injection of contrast agent, and then decreased rapidly to the signal intensity of normal scan at 20 min after injection.The SIR of right inguinal lymph nodes of BALB/c mice and Kunming mice were compared with those of Kunming mice before and after injection with F test. There was no significant difference in SIR between BALB/c mice and Kunming mice before and after injection (P 0.05).No significant adverse reactions were observed in all experimental mice after intradermal injection of Gd-DTPA.Conclusion it is safe and reproducible to use Gd-DTPA to perform interstitial Mr lymphography of mammary gland in mice. 2 Kunming mice can replace BALB/c mice in the study of interstitial MRL of mammary glands.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院附屬醫(yī)院放射科;北京老年醫(yī)院放射科;
【基金】：國家自然科學(xué)基金(面上:21575161;青年:21105120)
【分類號】：R445.2;R655.8

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