本文選題：慢性間歇性低氧　切入點(diǎn)：模型　出處：《山西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：本研究意在建立慢性間歇性低氧（CIH）兔模型，用二維、血流及組織多普勒超聲心動(dòng)圖觀察CIH早期（0～8周）新西蘭兔右心結(jié)構(gòu)、功能及血流動(dòng)力學(xué)的動(dòng)態(tài)變化。 方法：選用健康雄性新西蘭大白兔24只，建立CIH動(dòng)物模型，實(shí)驗(yàn)持續(xù)8周。于CIH模型制作開始0、1、2、4、6、8周應(yīng)用常規(guī)及組織多普勒超聲心動(dòng)圖測量右室結(jié)構(gòu)、收縮及舒張功能參數(shù)，分析其動(dòng)態(tài)變化趨勢。并于每次超聲檢查后隨機(jī)處死1只兔，分離心臟、右室，進(jìn)行HE染色，觀察右室心肌、肺動(dòng)脈、肺組織結(jié)構(gòu)的變化。 結(jié)果：右心結(jié)構(gòu)參數(shù)變化：與0周相比，RV、RA8周增大（P＜0.05），PA主干、RVWd各周無明顯變化（P＞0.05）；RV收縮功能參數(shù)：與0周相比，RVFAC8周增大（P＜0.05），TAPSE4、6、8周均增大（P＜0.05），RMPI4周減�。≒＜0.05），6、8周恢復(fù)至基礎(chǔ)狀態(tài)，ICT2、4周縮短（P＜0.05），6、8周恢復(fù)至基礎(chǔ)狀態(tài)，肺動(dòng)脈血流頻譜ET1、2周縮短（P＜0.05），4、6、8周恢復(fù)至基礎(chǔ)狀態(tài)，肺動(dòng)脈血流頻譜AT1、2、4周縮短（P＜0.05），6、8周恢復(fù)至基礎(chǔ)狀態(tài)；RV舒張功能參數(shù)變化：與0周相比，IRT于1、2周均縮短（P＜0.05），E/E’1、2周均減�。≒＜0.05），4、6、8周均恢復(fù)至基礎(chǔ)狀態(tài)，E/A4、6、8周增大（P＜0.05），E’/A’8周增大（P＜0.05）；④病理學(xué)變化:心肌細(xì)胞病理變化：細(xì)胞核肥大，染色加深，部分胞漿疏松淡染，呈水腫樣，，肌纖維有灶性壞死及溶解,心肌間質(zhì)增寬,局部細(xì)胞增多,其形態(tài)與成纖維細(xì)胞相似；肺組織病理變化：可見炎性細(xì)胞浸潤，毛細(xì)血管壁擴(kuò)張充血，大量漿液、纖維素滲出，肺小動(dòng)脈橫切面平滑肌細(xì)胞數(shù)增加，肺小動(dòng)脈血管壁略增厚，管腔略變窄。 結(jié)論：CIH早期新西蘭兔右室功能代償性增強(qiáng)，右心功能異常早于結(jié)構(gòu)異常；右室舒張功能代償早于收縮功能，IRT和ICT變化早于其它舒張及收縮功能參數(shù)。
[Abstract]:Objective: to establish a chronic intermittent hypoxia rabbit model and to observe the dynamic changes of the right heart structure, function and hemodynamics in New Zealand rabbits at the early stage of CIH by two-dimensional, flow and tissue Doppler echocardiography. Methods: 24 healthy male New Zealand white rabbits were used to establish CIH animal model for 8 weeks. The right ventricular structure, systolic and diastolic function parameters were measured by routine and tissue Doppler echocardiography at the beginning of CIH model making. After each ultrasound examination, a rabbit was killed at random to separate the heart and the right ventricle and stained with HE to observe the changes of the tissue structure of the right ventricle, pulmonary artery and lung. Results: compared with 0 week, the RV RV RA8 weeks increased P < 0.05 and PA trunk RVWd showed no significant change (P > 0.05). Compared with 0 week, RVFAC 8 weeks increased P < 0.05% TAPSE468 weeks increased P < 0.05% RMPI 4 weeks decreased P < 0.05 weeks, and recovered to the basic state ICT2 / 4 weeks, respectively (P < 0.05), P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05%, P < 0. 05, P > 0. 05%, respectively. Shortened P < 0.05 and restored to the basic state in 8 weeks. The pulmonary artery blood flow spectrum et _ (1) was shortened in 2 weeks, P < 0.05, and recovered to the basic state in 8 weeks. Pulmonary artery blood flow spectrum AT1 / 2 ~ 2 ~ 4 weeks shortened P < 0. 05 ~ 0. 05 ~ 6 ~ 8 weeks to return to basic state and RV diastolic function parameters changed: compared with 0 ~ (th) week, IRT was shortened at 12 ~ 2 weeks (P < 0. 05), P < 0. 05% E / E ~ (1 +) decreased in 2 ~ 2 weeks, P < 0. 05 ~ 0. 05 ~ 0. 05% EA4 / A ~ 6 ~ 8 weeks increased, P < 0. 05% E / A / A ~ (8) week increased, P < 0. 05% P < 0. 05% E / A ~ (8) week increased, P < 0. 05% P < 0. 05% P < 0. 05% P < 0. 05% P < 0. 05%. Cardiac myocyte pathological changes: nuclear hypertrophy, The staining deepened, some of the cytoplasm were loose and light stained, showing edema, focal necrosis and dissolution of muscle fibers, widening of myocardial interstitial cells and increasing of local cells, their morphology was similar to that of fibroblasts, pathological changes of lung tissue: inflammatory cell infiltration was observed. Capillary wall dilatation hyperemia, a large amount of serous, cellulose exudate, pulmonary arteriole cross-sectional smooth muscle cells increased, pulmonary arteriole wall slightly thickened, the lumen slightly narrow. Conclusion right ventricular function compensatory enhancement, right ventricular dysfunction is earlier than structural abnormality, right ventricular diastolic function compensation is earlier than systolic function, IRT and ICT changes are earlier than other parameters of diastolic and systolic function.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R540.45

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相關(guān)期刊論文 前6條

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