木霉菌是一類常見的真菌,廣泛分布于世界各地,在工業(yè)和農(nóng)業(yè)中具有顯著的經(jīng)濟(jì)價值。從內(nèi)蒙古自治區(qū)草原生態(tài)系統(tǒng)和森林生態(tài)系統(tǒng)采集的土壤中分離出木霉菌,然后對木霉菌種進(jìn)行鑒定,并對其區(qū)域分布、生物多樣性及潛在生物防治能力進(jìn)行評價。主要研究結(jié)果如下:1、內(nèi)蒙木霉菌資源多樣性。2014-2016年,從內(nèi)蒙古草原和森林生態(tài)系統(tǒng)共采集土樣711份,共分離得到417個木霉菌株,采用形態(tài)學(xué)和分子鑒定相結(jié)合的方法,共鑒定出21個已知木霉種:T.cf.harzianum（205）,T.longibrachiatum（112）,T.afroharzianum（27）,T.koningiopsis（16）,T.asperelloides（13）,T.hamatum（9）,T.rossicum（7）,T.citrinoviride（7）,T.alni（3）,T.konilangbra（2）,T.harzianum（3）,T.saturnisporum（1）,T.crassum（2）,T.velutinum（2）,T.guizhouense（1）,T.gamsii（2）,T.sinuosum,T.atroviridi... 

【文章頁數(shù)】：231 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 GENERAL INTRODUCTION
    1.1 RESEARCH BACKGROUND
    1.2 LITERATURE REVIEW
        1.2.1 Trichoderma
        1.2.2 Taxonomy of Trichoderma
        1.2.3 Morphology of Trichoderma
        1.2.4 Identification of species based on the analysis of DNA sequences
        1.2.5 Internal Transcribed Spacer (ITS)
        1.2.6 Roles of Trichoderma as Biological Control Agent
        1.2.7 Trichoderma-plant Interactions
    1.3 RESEARCH AIM AND OBJECTIVES
        1.3.1 Aim
    1.4 FLOW CHART OF OUR STUDY
CHAPTER 2 BIODIVERSITY OF THE GENUS HYPOCREA / TRICHODERMA IN DIFFERENT GRASSLAND AND FOREST SOIL ECOSYSTEMS OF INNER MONGOLIA, NORTHERN CHINA
    2.1 INTRODUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Sample sites and sampling procedure
        2.2.2 Isolation of Trichoderma spp
        2.2.3 Stock Culture
        2.2.4 Morphological Characterization and Growth Rate
        2.2.5 Molecular Analysis
    2.3 RESULTS
        2.3.1 Species identification
        2.3.2 Morphological Characterization
        2.3.3 Growth rate
        2.3.4 Molecular Analysis
        2.3.5 Phylogenetic analysis
        2.3.6 New species Descriptions
        2.3.7 Diversity of Trichoderma/ Hypocrea isolates in Inner Mongolia
    2.4 DISCUSSION
CHAPTER 3 DIVERSITY OF TRICHODERMA ISOLATES FROM INNER MONGOLIA SOIL ON BIOLOGICAL CONTROL POTENTIAL
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Trichoderma Strains Used
        3.2.2 Collection of pathogens
        3.2.3 In vitro antagonistic tests
        3.2.4 Evaluation on biocontrol and plant growth promotion in vivo
        3.2.5 Statistical analysis
    3.3 RESULT
        3.3.1 In vitro Screening for Antagonistic Potential of Twenty-one Species of Trichoderma against Different Plant Pathogenic Fungi
        3.3.2 Screening of indigenous potential antagonistic Trichoderma strains from Inner Mongolia soil against selected phytopathogenic fungi
        3.3.3 Analysis of the Antagonistic effect for T.cf harzianum on selected phytopathogenic fungi
        3.3.4 Biocontrol efficacy of Trichoderma spp. against wilt of cucumber caused by Fusarium oxysporum f.sp. cucumerinum under In vivo conditions
        3.3.5 Effect of treating cucumber seeds with Trichoderma isolates on the severity of Fusarium wilt disease
        3.3.6 Biocontrol Potential of Local Trichoderma isolates from Inner Mongolia based on their isolation regions and ecosystem
        3.3.7 Biocontrol efficacy of T.cf. harzianum against wilt disease of Cucumber caused by F. oxysporum f.sp. cucumerinum
    3.4 DISCUSSION
        3.4.1 Biocontrol potential of the isolated strains
CHAPTER 4 DIVERSITY OF LYTIC ENZYMATIC ACTION OF TRICHODERMA ISOLATES SELECTED BASED ON BIOLOGICAL CONTROL PERFORMANCE
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Qualitative screening of Extra Cellular Enzyme
        4.2.2 Quantitative estimations of Extra Cellular Enzyme
    4.3 RESULT
        4.3.1 In vitro antifungal activity
        4.3.2 Determination of different biocontrol enzymatic activity
        4.3.3 Spectrophotometric determination of Enzyme activity of Trichoderma isolates
    4.4 DISCUSSIONS
CHAPTER 5 OVERALL CONCLUSION AND RECOMMENDATIONS
    5.1 CONCLUSION
    5.2 RECOMMENDATION
ACKNOWLEDGEMENTS
RESUME
Publications
REFERENCES
Appendix



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