鼻氣管鳥桿菌（Omithobacterium rhinotracheale, ORT）是一種與多種禽類,特別是雞的呼吸道疾病相關(guān)的革蘭氏陰性菌,廣泛分布于世界各地,給禽類產(chǎn)業(yè)造成了一定的經(jīng)濟損失。對于該菌的致病機制及流行病學尚缺乏系統(tǒng)的研究,特別是我國禽類的感染和流行情況的研究甚少。本研究從不同地區(qū)321份患有臨床呼吸道疾病的禽類的氣管樣品中分離到27株的ORT,其中21株（77.8%）在首次接種時即分離到小菌落變異株（small colony variant, SCV）,經(jīng)過連續(xù)傳代,從中挑選出5株穩(wěn)定的小菌落變異株及其對應的親代菌株進行了研究。采用API-20NE進行生化鑒定,結(jié)果表明5株ORT小菌落變異株的生化反應均為0020000型,而與之相對應的親代菌株則出現(xiàn)兩種不同生化反應結(jié)果：ORT-WT3、ORT-WT13、ORT-WT21和ORT-WT28為0020004型,ORT-WT16為0220004型。五株小菌落變異株及親代菌株的p-半乳糖苷酶檢測結(jié)果為陽性,親代菌株氧化酶實驗結(jié)果為陽性,而小菌落變異株的結(jié)果為陰性。體外培養(yǎng)試驗表明,小菌落變異株較其和親代菌株生長速度慢,遲緩... 

【文章來源】：中國農(nóng)業(yè)大學北京市 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：93 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
Contents
Abbreviation
Index of figures
Index of tables
Introduction
    Ornithobacterium rhinotracheale
        History
        Etiology Classification,Name and Synonyms
        Isolation and Identification
        Growth Requirements
        Morphology and Stainin
        Biochemical idenitifcaiton
        Strain Classificaiton
        Serological
        Molecular
        Experimental Infection
        Mode of Infection
        Pathogenicity
        O.rhinotracheale infections
        Diagnosis
        Serological Examination for Antibody Detection
        Epidemiological Investigations
        Treatment
        Vaccination
    Small colony variants
        History
        Auxotrophic phenotypes of SCVs
        Electron-transport-defective SCVs
        Thymidine-biosynthetic-defective SCVs
        The clinical presentation of SCV infections
        Isolating and identifying SCVs in clinical microbiology laboratory
        The pathogenesis of SCV infections
Virulence in animal models
    Aim of the studies described in this thesis
Material and methods
    Samples collection
    Isolation and identification of ORT-WTs and ORT-SCVs
    PCR and sequence analysis
    Biochemical characteristics
    Bacterial growth curve and enrichment on broth media
    Hemagglutination assay(HA)
    Auxotrophism assay
    Antimicrobial Susceptibility Testing
    Autolysis Assay
    Preparation of ORTs isolates for the assay
    Intracellular Persistence assay
Results
    Isolation of ORT
    Cultivation and morphological characteristics of ORT-WTs and ORT-SCVs
    Biochemical characteristics
    Bacterial Growth Curves
    Hemagglutination assay
    Antimicrobial Susceptibility Testing
    Autolysis Assay
    Intracellular persistence assay
    Phylogenetic analyzes
Discussion
Summary
Conclusion
References
Acknowledgement
Curriculum vitae
    Education and Qualifications
    Employment History
    Academic Papers
    Interests and Activities



本文編號：3474343