牛心樸子屬蘿藦科植物,廣泛分布于中國(guó)北方牧區(qū)。作為中草藥,臨床上主要用于抗炎。其活性成分包括半邊蓮屬堿或類生物堿類、黃酮類、多醣、萜烯、脂肪酸、磷脂（類）、氨基酸和維生素等,其中生物堿類是目前研究的主要內(nèi)容,并且牛心樸子中已明確的生物堿有13種。本研究對(duì)牛心樸子中生物堿類粗提物進(jìn)行了提取,并對(duì)其方法進(jìn)行了探討；就牛心樸子全草粉碎物不同劑量（12.5%、25%和50%飼料）對(duì)家兔的毒性進(jìn)行了研究,對(duì)其體征變化、存活率、血液生化指標(biāo)、組織器官病理變化進(jìn)行了觀察；以牛心樸子主要生物堿TMPD灌胃大鼠,研究了TMPD不同劑量（38.5mg/kg、77mg/kg、115.5mg/kg、154.8mg/kg和231.8mg/kg體重）對(duì)大鼠組織學(xué)、血液學(xué)及生物化學(xué)等生理生化功能的影響。結(jié)果顯示,通過(guò)研究,建立了以鹽酸-水-酒精為溶媒的牛心樸子生物堿的提取方法,生物堿的提取率可達(dá)0.23%。家兔出現(xiàn)運(yùn)動(dòng)障礙、采食量減少、體重減輕,甚至機(jī)體衰竭而死亡。血液白細(xì)胞增多,貧血,白蛋白減少。肝臟匯管區(qū)小葉間隔纖維化、膽管瘀血。腎臟皮質(zhì)小管空泡化增加。心臟出現(xiàn)退行性空泡狀心肌細(xì)胞,肺泡擴(kuò)張,在231.8mg/... 

【文章來(lái)源】：中國(guó)農(nóng)業(yè)科學(xué)院北京市

【文章頁(yè)數(shù)】：111 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
博士學(xué)位論文評(píng)閱人、答辯委員會(huì)簽名表
Abstract
摘要
Abbreviation
CHAPTER 1 INTRODUCTION
    1.1 GENERAL INTRODUCTION
    1.2 Biological activities of phenanthroindolizidine alkaloids
    1.3 Biological activities of piperidine alkaloids
    1.4 Methods of extraction alkaloids
    1.5 Histological and biochemical effects of plants containing alkaloids
    1.6 Genotoxic effects of alkaloids
    1.7 Aim of study
CHAPTER 2 EXTRATION OF ALKALOIDS FROM C KOMAROVII
    2.1 INTRODUCTION
    2.2 MATTERIALS AND METHODS
        2.2.1 plant matterial
        2.2.2 solvents
        2.2.3 method of extraction
        2.2.4 method of detection alkaloid in extracting matterial
    2.3 RESULTS AND DISCUSSION
    2.4 CONCLUSION
CHAPTER 3 BIOCHEMICAL, HEMATOLOGICAL ANDHISTOPATHOLOGICAL EFFECT OF C KOMAROVIIAL.ILJINSKI ON RABBITS
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Collection,identification and preperation of plants materials
        3.2.2 Animal and experimental design
        3.2.3 Blood and tissue collection
        3.2.4 Biochemical and hematological analysis
        3.2.5 Histopathological analysis
            3.2.5.1 Paraffin section techniques
            3.2.5.2 Hematoxylin and eosin stain technique
        3.2.6 special stain
            3.2.6.1 Trichrmoe stain (Masson)
            3.2.6.2 Gomori(Gordon and Sweat)stains
        3.2.7 Histopathological examination
        3.2.8 Statistical analysis
    3.3 RESULTS
        3.3.1 Clinical signs
        3.3.2 Effects of plants on rabbit food consumption
        3.3.3 Effects of plants on weight of animals
        3.3.4 Biochemical analysis results
        3.3.5 Hematological analysis results
        3.3.6 Histopathological results
    3.4 DISSCUSION
    3.5 CONCLUSION
CHAPTER 4 BIOCHEMICAL,HEMATOLOGICAL ANDHISTOPATHOLOGICAL EFFECT OF 2,2,6,6-TETRAMETHYL-4-PIPERIDONE ON RATS
    4.1 INTRODUCTION
    4.2 MATERIALSAND METHODS
        4.2.1 Chemicals matterial
        4.2.2 Collection and maintenance of animals
        4.2.3 Determination of median lethal dose(LD50)
        4.2.4 Experimental design
        4.2.5 Biochemical and hematological analysis
        4.2.6 Histopathological examination
        4.2.7 Molecular analysis
            4.2.7.1 Animal and sample collection
            4.2.7.2 RNA extraction and RT-PCR
            4.2.7.3 Real time RT-PCR of rats mRNA
        4.2.8 Statistical analysis
    4.3 RESULTS
        4.3.1 Clinical signs
        4.3.2 Effects of TMPD on weight of treated rats
        4.3.3 Biochemical and hematological assay
            4.3.3.1 Effects of TMPD on rats treated for one week
            4.3.3.2 Effects of TMPD on rats treated for two week
        4.3.4 Histopathological results
        4.3.5 Bcl211,Bcl2,cytochrome C and FAAD protein expression
    4.4 DISSCUSION
    4.5 CONCLUSION
CHAPTER 5 GENERAL CONCLUSION
REFERENCE
ACKNOWLEDGEMENT
RESUME



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