【摘要】：為研究硫酸化銀耳多糖(sTPS_(70c))和硫酸化黨參多糖(sCPPS_(50c))增強免疫作用機理,本試驗以未修飾銀耳多糖(TPStp)為對照,采用噻唑藍(MTT)法和實時熒光定量PCR法測定了sTPS_(70c)和sCPPS_(50c)對雞T淋巴細胞增殖及白細胞介素-2(IL-2)mRNA表達水平的影響。結果表明,多糖單獨加入到外周血淋巴細胞時,sTPS_(70c)和sCPPS_(50c)幾乎所有濃度均可顯著刺激T淋巴細胞的增殖(P0.05),而TPStp僅在濃度為3.125μg/mL時顯著刺激T淋巴細胞增殖(P0.05);多糖與植物血凝素P(PHA-P)同時加入到外周血淋巴細胞時,sCPPS_(50c)在濃度為0.3911.563μg/mL時顯著刺激T淋巴細胞增殖(P0.05);sTPS_(70c)和sCPPS_(50c)在濃度為1.563μg/mL時可提高T淋巴細胞IL-2 mRNA的表達水平(P0.05),其中sTPS_(70c)對IL-2 mRNA的促表達作用顯著強于TPS_(tp)(P0.05),且在濃度為1.563μg/mL時的作用最強。結果提示,硫酸化修飾可以提高多糖的淋巴細胞增殖活性及顯著增強IL-2 mRNA的表達,且以sTPS_(70c)的作用較強,這與取代度有一定的相關性。
[Abstract]:In order to study the immune enhancement mechanism of Tremella sulfated polysaccharide (sTPS_ (70c) and Codonopsis pilosula polysaccharide (sCPPS_ (50c), the effects of sTPS_ (70c) and sCPPS_ (50c) on the proliferation of chicken T lymphocytes and the expression of IL-2 (IL-2) mRNA were measured by thiazolyl blue (MTT) and real-time fluorescence quantitative PCR. The results showed that when polysaccharides were added to peripheral blood lymphocytes alone, almost all concentrations of sTPS_ (70c) and sCPPS_ (50c) could significantly stimulate the proliferation of T lymphocytes (P 0.05), while TPStp only stimulated the proliferation of T lymphocytes at the concentration of 3.125 渭 g / mL (P 0.05). When polysaccharide and plant hemagglutinin P (PHA-P) were added to peripheral blood lymphocytes at the concentration of 0.3911.563 渭 g / mL, sCPPS_ (50c) significantly stimulated the proliferation of T lymphocytes (P 0.05). When the concentration of sTPS_ (70c) and sCPPS_ (50c) was 1.563 渭 g / mL, the expression of IL-2 mRNA in T lymphocytes was increased (P 0.05), and the effect of sTPS_ (70c) on the expression of IL-2 mRNA was significantly stronger than that of TPS_ (tp) (P 0.05), and the effect was the strongest at the concentration of 1.563 渭 g / mL. The results suggest that sulfation modification can increase the proliferation activity of polysaccharide lymphocytes and significantly enhance the expression of IL-2 mRNA, and the effect of sTPS_ (70c) is strong, which is related to the degree of substitution.
【作者單位】： 蕪湖職業(yè)技術學院生物工程學院;南京農業(yè)大學動物醫(yī)學院;
【基金】：安徽省教育廳自然科學重點項目(KJ2017A564);安徽省教育廳自然科學重點項目(KJ2015A401) 校級自然科學研究項目“應用復方中草藥防治豬場常見呼吸道疾病的研究”(wzyzr201619) 校級“教學質量與教學改革工程”項目(2016)
【分類號】：S831.5

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