【摘要】：旨在探討長期飼喂高脂飼料對大鼠血脂、肝及腸道菌群的影響。本研究選擇成年健康SD大鼠隨機(jī)分為兩組:空白對照組和高脂飼料組(n=10),分別采用維持飼料和高脂飼料飼喂13周,在第8及第13周,采集血清進(jìn)行血脂分析,并于第13周采集肝組織及盲腸內(nèi)容物,檢測肝病變及腸道菌群變化,并預(yù)測與其相關(guān)的代謝通路。結(jié)果表明,高脂飼料可顯著升高TG、TCH及LDL水平(P0.01),并導(dǎo)致嚴(yán)重的肝脂肪變性;相比于第8周結(jié)果,延長飼喂時間可顯著增高TCH和HDL(P0.01)。高脂飼料可顯著降低腸道菌群Shannon及Chao指數(shù)(P0.001),降低菌群多樣性,并顯著改變菌群組成,引起厚壁菌門及放線菌門的增加和擬桿菌門的減少(P0.01);PICRUSt預(yù)測對照組差異代謝通路,主要涉及細(xì)菌的生存與繁殖等,而高脂飼料組主要與能量、氨基酸代謝相關(guān),這些通路的改變可能與血脂異常及腸道菌群多樣性的降低緊密聯(lián)系。本研究證實(shí)了長期飼喂高脂飼料對大鼠血脂、肝及腸道菌群的危害,為血脂異常成因的闡明提供了依據(jù),也對寵物健康飲食管理有一定的指導(dǎo)作用。
[Abstract]:The aim of this study was to investigate the effects of high fat diet on blood lipids, liver and intestinal flora in rats. In this study, adult healthy SD rats were randomly divided into two groups: blank control group and high fat diet group (nr10). The rats were fed with maintenance diet and high fat diet for 13 weeks, and serum lipid levels were analyzed at the 8th and 13th weeks. The contents of liver tissue and cecum were collected at the 13th week to detect the changes of hepatic lesions and intestinal flora and to predict the metabolic pathways associated with them. The results showed that high fat diet could significantly increase the levels of TG,TCH and LDL (P0.01) and lead to severe hepatic steatosis, and the prolongation of feeding time could significantly increase TCH and HDL (P0.01) compared with the results of the 8th week. High fat diet significantly decreased the Shannon and Chao index (P0.001) of intestinal flora, decreased the diversity of flora, and significantly changed the composition of flora, resulting in the increase of phylum and actinomycetes and the decrease of Bacteroides (P0.01). PICRUSt predicted that the differential metabolic pathways in the control group were mainly involved in the survival and reproduction of bacteria, while those in the high-fat diet group were mainly related to the metabolism of energy and amino acids. The changes of these pathways may be closely related to the abnormal blood lipid and the decrease of the diversity of intestinal flora. This study confirmed the harm of long-term feeding high fat diet on blood lipids, liver and intestinal microflora in rats, provided the basis for elucidating the causes of dyslipidemia, and also had a certain guiding effect on the management of healthy diet for pets.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院蘭州畜牧與獸藥研究所農(nóng)業(yè)部獸用藥物創(chuàng)制重點(diǎn)實(shí)驗(yàn)室甘肅省新獸藥工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(31402254;31572573) 甘肅省青年科技基金計(jì)劃(1506RJYA148)
【分類號】：S816

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