【摘要】：旨在探討長期飼喂高脂飼料對大鼠血脂、肝及腸道菌群的影響。本研究選擇成年健康SD大鼠隨機分為兩組:空白對照組和高脂飼料組(n=10),分別采用維持飼料和高脂飼料飼喂13周,在第8及第13周,采集血清進行血脂分析,并于第13周采集肝組織及盲腸內(nèi)容物,檢測肝病變及腸道菌群變化,并預(yù)測與其相關(guān)的代謝通路。結(jié)果表明,高脂飼料可顯著升高TG、TCH及LDL水平(P0.01),并導(dǎo)致嚴重的肝脂肪變性;相比于第8周結(jié)果,延長飼喂時間可顯著增高TCH和HDL(P0.01)。高脂飼料可顯著降低腸道菌群Shannon及Chao指數(shù)(P0.001),降低菌群多樣性,并顯著改變菌群組成,引起厚壁菌門及放線菌門的增加和擬桿菌門的減少(P0.01);PICRUSt預(yù)測對照組差異代謝通路,主要涉及細菌的生存與繁殖等,而高脂飼料組主要與能量、氨基酸代謝相關(guān),這些通路的改變可能與血脂異常及腸道菌群多樣性的降低緊密聯(lián)系。本研究證實了長期飼喂高脂飼料對大鼠血脂、肝及腸道菌群的危害,為血脂異常成因的闡明提供了依據(jù),也對寵物健康飲食管理有一定的指導(dǎo)作用。
[Abstract]:The aim of this study was to investigate the effects of high fat diet on blood lipids, liver and intestinal flora in rats. In this study, adult healthy SD rats were randomly divided into two groups: blank control group and high fat diet group (nr10). The rats were fed with maintenance diet and high fat diet for 13 weeks, and serum lipid levels were analyzed at the 8th and 13th weeks. The contents of liver tissue and cecum were collected at the 13th week to detect the changes of hepatic lesions and intestinal flora and to predict the metabolic pathways associated with them. The results showed that high fat diet could significantly increase the levels of TG,TCH and LDL (P0.01) and lead to severe hepatic steatosis, and the prolongation of feeding time could significantly increase TCH and HDL (P0.01) compared with the results of the 8th week. High fat diet significantly decreased the Shannon and Chao index (P0.001) of intestinal flora, decreased the diversity of flora, and significantly changed the composition of flora, resulting in the increase of phylum and actinomycetes and the decrease of Bacteroides (P0.01). PICRUSt predicted that the differential metabolic pathways in the control group were mainly involved in the survival and reproduction of bacteria, while those in the high-fat diet group were mainly related to the metabolism of energy and amino acids. The changes of these pathways may be closely related to the abnormal blood lipid and the decrease of the diversity of intestinal flora. This study confirmed the harm of long-term feeding high fat diet on blood lipids, liver and intestinal microflora in rats, provided the basis for elucidating the causes of dyslipidemia, and also had a certain guiding effect on the management of healthy diet for pets.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院蘭州畜牧與獸藥研究所農(nóng)業(yè)部獸用藥物創(chuàng)制重點實驗室甘肅省新獸藥工程重點實驗室;
【基金】：國家自然科學(xué)基金(31402254;31572573) 甘肅省青年科技基金計劃(1506RJYA148)
【分類號】：S816

【參考文獻】

相關(guān)期刊論文 前9條

1 黃紅麗;周永健;鄭啼嬰;聶玉強;杜艷蕾;;腸道菌群在非酒精性脂肪性肝病大鼠中的變化及意義[J];廣東醫(yī)學(xué);2016年09期

2 羅朵生;李坤平;樸勝華;郭姣;;高脂血癥大鼠造模過程中血液代謝組學(xué)研究[J];中藥新藥與臨床藥理;2016年01期

3 朱超霞;倉楨;加孜熱亞·再依拿提;李琴;夏芳珍;陸穎理;;鹽酸小檗堿對高脂飲食誘導(dǎo)的非酒精性脂肪性肝病大鼠腸道菌群的影響[J];上海交通大學(xué)學(xué)報(醫(yī)學(xué)版);2015年04期

4 葉妍琳;白正廣;張斌愷;張海燕;王廣龍;肖嘯;;犬、貓肥胖癥病因分析及管理[J];上海畜牧獸醫(yī)通訊;2015年01期

5 Qiong Wu;Hai Zhang;Xin Dong;Xiao-Fei Chen;Zhen-Yu Zhu;Zhan-Ying Hong;Yi-Feng Chai;;UPLC-Q-TOF/MS based metabolomic profiling of serum and urine of hyperlipidemic rats induced by high fat diet[J];Journal of Pharmaceutical Analysis;2014年06期

6 孟良艷;陳秀琴;石達友;黃海定;郭世寧;;四君子湯對脾虛大鼠腸道菌群多樣性的影響[J];畜牧獸醫(yī)學(xué)報;2013年12期

7 周云楓;李沙;蘇文;黃世錚;蒲丹;管又飛;;不同脂肪含量的高脂純化配方飼料對大、小鼠代謝綜合征發(fā)生的影響[J];基礎(chǔ)醫(yī)學(xué)與臨床;2012年03期

8 滿都日娃;馬利芹;利凱;楊永紅;;犬、貓肥胖癥的防治[J];河北北方學(xué)院學(xué)報(自然科學(xué)版);2009年03期

9 陳眷華;盧占軍;徐在品;鄧小燕;毛以智;霍欣;;大黃醇提液抗家兔實驗性動脈粥樣硬化作用的病理形態(tài)學(xué)研究[J];畜牧獸醫(yī)學(xué)報;2007年07期

【共引文獻】

相關(guān)期刊論文 前10條

1 馬寧;劉希望;孔曉軍;李世宏;秦哲;焦增華;楊亞軍;李劍勇;;長期飼喂高脂飼料對大鼠血脂、肝及腸道菌群的影響[J];畜牧獸醫(yī)學(xué)報;2017年07期

2 何毅芳;楊欽河;張玉佩;鄧遠軍;梁曙;陳妍凝;;從肝脾相關(guān)理論探討中醫(yī)藥防治慢性肝病的思路與方法[J];中醫(yī)學(xué)報;2017年07期

3 肖翠霞;吳科銳;韓凌;;中醫(yī)藥對腸道菌群的影響研究進展[J];中成藥;2017年06期

4 邢宇;劉鑫;林園;張勇;;小檗堿藥理作用及其臨床應(yīng)用研究進展[J];中國藥理學(xué)與毒理學(xué)雜志;2017年06期

5 馬堯;姜維娜;江紅;;2型糖尿病伴高脂血癥患者氧化應(yīng)激反應(yīng)與血脂代謝紊亂的關(guān)系研究[J];臨床和實驗醫(yī)學(xué)雜志;2017年11期

6 付蓉;榮向路;郭姣;;復(fù)方貞術(shù)調(diào)脂方對非酒精性脂肪肝的肝PPARα及其下游基因的影響[J];中國中西醫(yī)結(jié)合雜志;2017年06期

7 哈申圖雅;孟根杜希;云蘇日娜;張沖;敖德德;李姹潤;;蒙藥達日布-8對動脈粥樣硬化兔內(nèi)皮祖細胞的影響[J];中華中醫(yī)藥雜志;2017年06期

8 張海博;王宇;;非酒精性脂肪肝的現(xiàn)代研究進展[J];海南醫(yī)學(xué);2017年10期

9 范紅晶;虞立;金偉鋒;李敏;周靜;李曉紅;張宇燕;;原兒茶醛與羥基紅花黃色素A單用與合用在高脂血癥大鼠體內(nèi)的藥動學(xué)-藥效學(xué)相關(guān)性研究[J];中國中藥雜志;2017年13期

10 王璐璇;劉sズ，

本文編號：2423130