日糧精氨酸的添加對(duì)肉雞先天性免疫和抗氧化能力調(diào)節(jié)作用的研究
發(fā)布時(shí)間：2019-01-10 18:45
【摘要】：本論文研究了日糧精氨酸添加對(duì)肉雞先天性免疫功能和抗氧化能力的影響。探討了精氨酸調(diào)節(jié)肉雞免疫功能的機(jī)制,為畜禽生產(chǎn)優(yōu)質(zhì)肉雞提供基礎(chǔ)數(shù)據(jù)。同時(shí)對(duì)精氨酸在肉雞生產(chǎn)中的合理應(yīng)用提供重要的理論指導(dǎo)意義。本試驗(yàn)共分為兩個(gè)部分：試驗(yàn)一探討了日糧精氨酸的添加對(duì)肉雞先天性免疫功能的影響。本試驗(yàn)選擇1日齡愛拔益加(AA)商品肉雞192只,隨機(jī)分成4個(gè)處理組,每組8個(gè)重復(fù),每個(gè)重復(fù)6只雞,試驗(yàn)采用單因素完全隨機(jī)設(shè)計(jì),包含4個(gè)精氨酸水平(10.0、15.0、20.0、25.0g/kg),分別在第1、14、和21天統(tǒng)計(jì)生產(chǎn)性能。于21d采集法氏囊、脾臟、盲腸扁桃體和血清用于實(shí)時(shí)熒光定量PCR檢測(cè)、酶聯(lián)免疫檢測(cè)以及淋巴細(xì)胞分離。本試驗(yàn)結(jié)果顯示,隨著日糧精氨酸的添加極顯著提高了(P0.001)肉雞14d的體重,肉雞體增重呈現(xiàn)二次曲線增長(P0.001),顯著提高了(P0.05)肉雞14-21d的采食量,料肉比呈現(xiàn)線性下降的趨勢(shì)(P0.001)。隨著日糧精氨酸的添加線性提高了盲腸扁桃體MIP-1β mRNA表達(dá)量(P0.001),顯著影響了肉雞盲腸扁桃體IFN-γ mRNA表達(dá)量,但是未呈現(xiàn)出線性或二次曲線趨勢(shì)。低水平(10g/kg)和高水平(25g/kg)精氨酸添加組相比,25g/kg的精氨酸添加顯著提高了肉雞盲腸扁桃體IFN-γ的mRNA表達(dá)量。對(duì)肉雞盲腸扁桃體的TLR5 mRNA表達(dá)量無顯著性差異(P0.05)。隨著日糧精氨酸水平的升高,肉雞脾臟IL-1p的mRNA表達(dá)量呈現(xiàn)出二次曲線變化趨勢(shì)(P=0.015)。同時(shí)顯著影響了IL-18的mRNA表達(dá)量,并呈現(xiàn)出線性和二次曲線變化趨勢(shì)(P0.05),IL-6的mRNA表達(dá)量呈現(xiàn)出二次曲線變化趨勢(shì)(P0.05)。隨著日糧精氨酸水平的提高,IgG和IgM濃度呈現(xiàn)出線性下降趨勢(shì)(P0.05),血清中IgA濃度未出現(xiàn)顯著性差異(P0.05),但隨著日糧精氨酸水平的增加,血清IgA濃度呈現(xiàn)上升的趨勢(shì)。于21d分離法氏囊細(xì)胞,進(jìn)行淋巴細(xì)胞分群,細(xì)胞增殖,和細(xì)胞周期以及細(xì)胞凋亡的檢測(cè)。試驗(yàn)結(jié)果表明：隨著日糧精氨酸水平的升高,細(xì)胞壞死率呈現(xiàn)出線性下降趨勢(shì)(P0.05),晚期凋亡細(xì)胞與壞死細(xì)胞比例呈現(xiàn)線性下降趨勢(shì)(P=0.003),日糧精氨酸的添加顯著增加了存活細(xì)胞的數(shù)量(P0.05),早期凋亡細(xì)胞呈現(xiàn)遞增趨勢(shì)(P0.05)。試驗(yàn)二探討了日糧精氨酸的添加對(duì)肉雞抗氧化能力調(diào)節(jié)作用的研究。試驗(yàn)設(shè)計(jì)同試驗(yàn)一。于21d采集肝臟用于過氧化氫酶、溶菌酶、過氧化物酶、總超氧化物歧化酶、丙二醛以及谷胱甘肽過氧化氫酶的測(cè)定。試驗(yàn)結(jié)果表明,隨著日糧精氨酸水平的添加,肉雞肝臟中GSH-Px活力線性增加(P0.05),而日糧精氨酸的添加并未對(duì)肉雞肝臟中MDA含量產(chǎn)生影響,而隨著日糧精氨酸水平的升高,肉雞肝臟中CAT活力線性增加(P=0.07),肝臟中溶菌酶含量出現(xiàn)顯著性差異,并隨著精氨酸水平的升高溶菌酶含量也隨之增加(P=0.001)。然而日糧精氨酸水平的增加并未對(duì)肝臟SOD、POD活力產(chǎn)生影響(P0.05)。綜上所述,日糧中添加高水平的精氨酸可能潛在的抑制肉雞的先天性免疫功能,同時(shí)日糧精氨酸添加能夠增強(qiáng)肉雞的抗氧化能力。
[Abstract]:The effects of dietary arginine on the innate immune function and the anti-oxidation ability of broiler chickens were studied in this paper. The mechanism of the regulation of the immune function of the broiler chickens was discussed, and the basic data was provided for the production of high-quality broiler chickens. and also provides important theoretical guiding significance for the reasonable application of the arginine in the production of the broiler. The experiment was divided into two parts: one study of the effect of the addition of arginine on the first-day immune function of the broiler. In this study, 192 broilers were randomly divided into 4 treatment groups, each group was divided into 4 treatment groups, each group was divided into 4 treatment groups, each group of 6 chickens was designed with single factor, and four arginine levels (10. 0, 15. 0, 20. 0, 25. 0g/ kg) were selected, and the production performance was counted at day 1, 14, and 21, respectively. The bursa of Fabricius, the spleen, the tonsil and the serum of the cecum were collected for real-time fluorescence quantitative PCR, enzyme-linked immunosorbent assay and lymphocyte separation. The results showed that, with the increase of dietary arginine (P. 001), the body weight of broiler (P. 001) and body weight of broiler were increased (P 0.001), and the feed intake of 14-21d in broiler was significantly improved (P 0.01), and the ratio of feed to meat decreased linearly (P 0.001). With the addition of day-to-day arginine, the expression of MIP-1 mRNA in the cecum tonsils was increased linearly (P 0.001), and the expression of IFN-1 mRNA in the cecum of the cecum was significantly affected, but there was no linear or quadratic trend. At a low level (10 g/ kg) and a high level (25g/ kg) of the arginine addition group, the addition of 25g/ kg of arginine significantly increased the mRNA expression of the cecum tonsils of the cecum of the broiler. There was no significant difference in the expression of TLR5 mRNA in the cecum tonsils (P0.05). The expression of IL-1p in the spleen of the broiler chickens showed a quadratic curve with the increase of the level of arginine (P = 0.015). At the same time, the mRNA expression of IL-18 was significantly affected, and the change trend of the linear and secondary curve was presented (P0.05), and the expression of IL-6 mRNA in the second curve (P0.05). The concentration of IgG and IgM in serum increased linearly with the increase of the level of arginine (P <0.05), but there was no significant difference in the concentration of IgA in the serum (P0.05). In the isolation of the bursa of Fabricius, the lymphocyte subpopulation, cell proliferation, cell cycle and cell apoptosis were detected. The results showed that with the increase of arginine level, the rate of cell necrosis showed a linear decrease (P <0.05), and the proportion of late apoptotic cells and necrotic cells decreased linearly (P = 0.003), and the addition of day-to-day arginine increased the number of viable cells (P0.05). The early apoptotic cells showed an increasing trend (P0.05). The effect of the addition of arginine on the anti-oxidation ability of broiler chickens was studied in this paper. The test design is the same as the test one. The liver is used for the determination of catalase, lysozyme, peroxidase, total superoxide dismutase, malondialdehyde and glutathione catalase at 21d. The results showed that the activity of GSH-Px in the liver of the broiler chickens increased linearly with the addition of the daily ration of arginine (P0.05), while the addition of the day-to-day arginine did not affect the content of MDA in the liver of the broiler. The activity of CAT in the liver of the broiler was increased linearly (P = 0.07), and the content of lysozyme in the liver was significantly different, and the content of lysozyme increased with the increase of the level of arginine (P = 0.001). However, the increase of arginine level did not affect the activity of SOD and POD in the liver (P0.05). To sum up, the addition of high levels of arginine in daily food may potentially inhibit the innate immune function of the broiler, while the addition of dietary arginine can enhance the anti-oxidation ability of the broiler.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831.5
[Abstract]:The effects of dietary arginine on the innate immune function and the anti-oxidation ability of broiler chickens were studied in this paper. The mechanism of the regulation of the immune function of the broiler chickens was discussed, and the basic data was provided for the production of high-quality broiler chickens. and also provides important theoretical guiding significance for the reasonable application of the arginine in the production of the broiler. The experiment was divided into two parts: one study of the effect of the addition of arginine on the first-day immune function of the broiler. In this study, 192 broilers were randomly divided into 4 treatment groups, each group was divided into 4 treatment groups, each group was divided into 4 treatment groups, each group of 6 chickens was designed with single factor, and four arginine levels (10. 0, 15. 0, 20. 0, 25. 0g/ kg) were selected, and the production performance was counted at day 1, 14, and 21, respectively. The bursa of Fabricius, the spleen, the tonsil and the serum of the cecum were collected for real-time fluorescence quantitative PCR, enzyme-linked immunosorbent assay and lymphocyte separation. The results showed that, with the increase of dietary arginine (P. 001), the body weight of broiler (P. 001) and body weight of broiler were increased (P 0.001), and the feed intake of 14-21d in broiler was significantly improved (P 0.01), and the ratio of feed to meat decreased linearly (P 0.001). With the addition of day-to-day arginine, the expression of MIP-1 mRNA in the cecum tonsils was increased linearly (P 0.001), and the expression of IFN-1 mRNA in the cecum of the cecum was significantly affected, but there was no linear or quadratic trend. At a low level (10 g/ kg) and a high level (25g/ kg) of the arginine addition group, the addition of 25g/ kg of arginine significantly increased the mRNA expression of the cecum tonsils of the cecum of the broiler. There was no significant difference in the expression of TLR5 mRNA in the cecum tonsils (P0.05). The expression of IL-1p in the spleen of the broiler chickens showed a quadratic curve with the increase of the level of arginine (P = 0.015). At the same time, the mRNA expression of IL-18 was significantly affected, and the change trend of the linear and secondary curve was presented (P0.05), and the expression of IL-6 mRNA in the second curve (P0.05). The concentration of IgG and IgM in serum increased linearly with the increase of the level of arginine (P <0.05), but there was no significant difference in the concentration of IgA in the serum (P0.05). In the isolation of the bursa of Fabricius, the lymphocyte subpopulation, cell proliferation, cell cycle and cell apoptosis were detected. The results showed that with the increase of arginine level, the rate of cell necrosis showed a linear decrease (P <0.05), and the proportion of late apoptotic cells and necrotic cells decreased linearly (P = 0.003), and the addition of day-to-day arginine increased the number of viable cells (P0.05). The early apoptotic cells showed an increasing trend (P0.05). The effect of the addition of arginine on the anti-oxidation ability of broiler chickens was studied in this paper. The test design is the same as the test one. The liver is used for the determination of catalase, lysozyme, peroxidase, total superoxide dismutase, malondialdehyde and glutathione catalase at 21d. The results showed that the activity of GSH-Px in the liver of the broiler chickens increased linearly with the addition of the daily ration of arginine (P0.05), while the addition of the day-to-day arginine did not affect the content of MDA in the liver of the broiler. The activity of CAT in the liver of the broiler was increased linearly (P = 0.07), and the content of lysozyme in the liver was significantly different, and the content of lysozyme increased with the increase of the level of arginine (P = 0.001). However, the increase of arginine level did not affect the activity of SOD and POD in the liver (P0.05). To sum up, the addition of high levels of arginine in daily food may potentially inhibit the innate immune function of the broiler, while the addition of dietary arginine can enhance the anti-oxidation ability of the broiler.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831.5
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