【摘要】：屎腸球菌(Enterococcus faecium)發(fā)酵飼料中的活菌數(shù)是保證其功能特性的關(guān)鍵因素,為了提高屎腸球菌發(fā)酵過(guò)程中的活菌總數(shù),本研究以屎腸球菌為試驗(yàn)菌株,選用4種不同特性的酵母浸出物來(lái)考察其對(duì)屎腸球菌活菌數(shù)的影響。通過(guò)單因素試驗(yàn),確定以酵母浸出物為唯一氮源培養(yǎng)屎腸球菌的活菌數(shù),根據(jù)酵母浸出物檢測(cè)指標(biāo)找出影響屎腸球菌活菌的關(guān)鍵因子。試驗(yàn)結(jié)果表明,培養(yǎng)基中主要提供生長(zhǎng)因子和微量元素的酵母浸出物對(duì)屎腸球菌的活性有明顯的影響,其中游離核苷酸的含量是影響屎腸球菌發(fā)酵活菌數(shù)重要因子,通過(guò)外源添加一定量的游離核苷酸,厭氧培養(yǎng)12 h,獲得的最終活菌數(shù)為176×10~8 CFU/m L,與未添加游離核苷酸相比,活菌數(shù)提高了將近40%。結(jié)果顯示,在培養(yǎng)基中添加一定量的游離核苷酸可提高屎腸球菌在發(fā)酵液中活菌數(shù)量,為工業(yè)化生產(chǎn)高活菌數(shù)的飼用屎腸球菌提供參考和借鑒。
[Abstract]:The number of live bacteria in the fermenting feed of Enterococcus faecium (Enterococcus faecium) is the key factor to ensure its functional characteristics. In order to increase the total number of live bacteria in the fermentation process of Enterococcus faecium, this study took Enterococcus faecium as the experimental strain. Four kinds of yeast extracts with different characteristics were used to study the effect of yeast extract on the number of live bacteria of Enterococcus faecium. According to the single factor test, the number of live bacteria of Enterococcus faecium cultured with yeast extract as the sole nitrogen source was determined, and the key factors affecting the living bacteria of Enterococcus faecium were found out according to the detection index of yeast extract. The results showed that the yeast extract, which mainly provided growth factors and trace elements, had a significant effect on the activity of Enterococcus faecium, and the content of free nucleotides was an important factor affecting the number of viable bacteria in fermentation of Enterococcus faecium. By adding a certain amount of free nucleotides and anaerobic culture for 12 h, the final viable bacteria number was 176 脳 10 ~ (8) CFU/m / L, which increased the number of live bacteria by nearly 40% compared with that without free nucleotides. The results showed that adding a certain amount of free nucleotides to the culture medium could increase the number of live bacteria of Enterococcus faecium in fermentation broth and provide a reference for the industrialized production of Enterococcus faecium with high number of live bacteria.
【作者單位】： 中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶生物技術(shù)研究所;湖北海宜生物科技有限公司;江南大學(xué)生物工程學(xué)院;
【基金】：中國(guó)熱帶農(nóng)業(yè)科學(xué)院基本科研業(yè)務(wù)費(fèi)基金(1630052016007、1630052016012、1630052016021)
【分類(lèi)號(hào)】：S816.3

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