【摘要】：牛傳染性鼻氣管炎(IBR)、牛病毒性腹瀉(BVD)和牛副流感3型(PI-3)是危害養(yǎng)牛業(yè)的三種重要病毒性傳染病,他們都能引起牛呼吸道疾病綜合征(BRDC),臨床表現(xiàn)為高熱、食欲下降、精神沉郁、呼吸困難、咳嗽、流涎、眼分泌物增多等,且廣泛分布于世界各地,給養(yǎng)牛業(yè)造成了巨大的經(jīng)濟(jì)損失,我國(guó)進(jìn)出境牛及其產(chǎn)品大多有對(duì)這三種疫病檢疫的要求。本文測(cè)定了在國(guó)外接種上述3種疫苗的進(jìn)口種牛的血清抗體效價(jià),評(píng)估了它對(duì)入境檢驗(yàn)檢疫工作的影響,提出了防控上述疾病的建議。研究結(jié)果如下:1.IBR血清抗體效價(jià)的測(cè)定及對(duì)IBR防控的建議本文采用ELISA、中和試驗(yàn)和PCR方法,測(cè)定了 126頭在國(guó)外接種滅活I(lǐng)BR疫苗的進(jìn)口種牛的血清抗體效價(jià)和病毒,分析了其對(duì)檢疫工作的影響,并提出了以接種能區(qū)分免疫和自然感染的基因缺失疫苗代替接種滅活常規(guī)疫苗的建議。2.BVD血清抗體效價(jià)的測(cè)定及對(duì)BVD防控的建議對(duì)126頭注射牛病毒性腹瀉疫苗的進(jìn)口牛群血清抗體效價(jià)進(jìn)行了測(cè)定,全面分析了其對(duì)后續(xù)檢疫工作的影響,進(jìn)而提出取消進(jìn)境種牛境外免疫BVDV疫苗的建議。3.PI-3血清抗體效價(jià)的測(cè)定及對(duì)PI-3防控的建議本文使用ELISA方法,對(duì)31頭注射牛副流感3型疫苗的進(jìn)口牛群血清抗體效價(jià)進(jìn)行測(cè)定,結(jié)合國(guó)內(nèi)外牛副流感3型疫苗的研究進(jìn)展,建議取消進(jìn)境種牛境外免疫BPIV3疫苗,并繼續(xù)研發(fā)更加有效的新型疫苗以防控該病的發(fā)生和傳播。綜上所述,本文全面分析了牛傳染性鼻氣管炎、牛病毒性腹瀉及牛副流感3型的防控措施,提出了中肯的建議,為我國(guó)從國(guó)外進(jìn)口種牛政策提供了新思路。
[Abstract]:Bovine infectious rhinotracheitis (IBR), bovine viral diarrhea (BVD) and bovine parainfluenza type 3 (PI-3) are three important viral infectious diseases which can cause bovine respiratory disease syndrome (BRDC),). Clinical manifestations include high fever, decreased appetite, depression, dyspnea, coughing, salivation, increased eye discharge, and widespread distribution around the world, causing enormous economic losses to the cattle industry. Most of China's imported and outgoing cattle and their products have quarantine requirements for these three diseases. In this paper, the titer of serum antibody of imported cattle inoculated with the above three kinds of vaccines abroad was determined, its influence on entry inspection and quarantine work was evaluated, and some suggestions for prevention and control of the above diseases were put forward. The results are as follows: determination of 1.IBR serum antibody titer and suggestions for IBR prevention and control. In this paper, the serum antibody titers and viruses of 126 imported cattle inoculated with inactivated IBR vaccine were determined by ELISA, neutralization test and PCR method. Its influence on quarantine work is analyzed. The suggestion of replacing the inactivated routine vaccine with the gene deletion vaccine which can distinguish immunity from natural infection was put forward. The titer of serum antibody of 2.BVD and the prevention and control of BVD for 126 vaccinated bovine viral diarrhea were suggested. The titers of serum antibodies of imported cattle were determined. In this paper, the influence of 3.PI-3 on the subsequent quarantine work was analyzed, and then the suggestion of canceling the BVDV vaccine from imported cattle was put forward. The titer of 3.PI-3 serum antibody and the suggestion of PI-3 prevention and control were determined in this paper, and the ELISA method was used in this paper. The titer of serum antibody against imported bovine parainfluenza type 3 vaccine was determined in 31 cattle injected with bovine parainfluenza 3 vaccine. Combined with the research progress of bovine parainfluenza type 3 vaccine at home and abroad, it was suggested that the BPIV3 vaccine of imported cattle should be cancelled. And continue to develop more effective new vaccines to prevent the occurrence and transmission of the disease. To sum up, the prevention and control measures of bovine infectious rhinotracheitis, bovine viral diarrhea and bovine parainfluenza type 3 are analyzed in this paper.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.23

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