【摘要】：對(duì)仙居雞和白耳黃雞2個(gè)蛋用型地方品種mtDNA D-loop區(qū)全序列進(jìn)行PCR擴(kuò)增和測(cè)序,分析其線粒體D-loop區(qū)多態(tài)性,并結(jié)合GenBank中洛島紅和白來(lái)航的D-loop區(qū)序列進(jìn)行進(jìn)化分析。結(jié)果表明:2個(gè)蛋用型地方品種mtDNA D-loop區(qū)全長(zhǎng)1 231~1 232bp,共發(fā)現(xiàn)18處單核苷酸變異位點(diǎn)和8種單倍型,核苷酸多樣性為0.006 51±0.001 09,單倍型多態(tài)性為0.781±0.011。系統(tǒng)發(fā)育分析發(fā)現(xiàn),2個(gè)蛋用型地方品種主要分布在A、B和C 3個(gè)進(jìn)化分支,B和C為優(yōu)勢(shì)分支,而2個(gè)標(biāo)準(zhǔn)品種則分布在A和E 2個(gè)進(jìn)化分支,E為優(yōu)勢(shì)分支。結(jié)果提示2個(gè)蛋用型地方品種遺傳多態(tài)性呈中等,與標(biāo)準(zhǔn)品種親緣關(guān)系較遠(yuǎn),母系來(lái)源也有所不同。這為2個(gè)品種的選育利用和有效保種提供遺傳背景資料和理論依據(jù)。
[Abstract]:The whole mtDNA D-loop region of Xianju chicken and white ear yellow chicken were amplified by PCR and sequenced, the polymorphism of mitochondrial D-loop region was analyzed, and the sequence of D-loop region of Luodaohong and Bailaihang in GenBank was analyzed. The results showed that the total length of mtDNA D-loop region was 1231 BP, 18 single nucleotide mutation sites and 8 haplotypes were found in 2 native egg varieties. The nucleotide diversity was 0.006 51 鹵0.001 09, and the haplotype polymorphism was 0.781 鹵0.011. Phylogenetic analysis showed that two native varieties of egg type were mainly distributed in three evolutionary branches of Agna B and C, B and C were dominant branches, while two standard varieties were distributed in two evolutionary branches of A and E, and E was the dominant branch. The results suggested that the genetic polymorphisms of the two native varieties were moderate, and the genetic relationship with the standard varieties was far away, and the matriarchal origin was also different. This provides genetic background data and theoretical basis for breeding, utilization and effective conservation of two varieties.
【作者單位】： 江蘇省家禽科學(xué)研究所;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(31372277;31501917;31672382)
【分類號(hào)】：S831.2

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