不同地區(qū)沙門菌的流行病學(xué)調(diào)查及腸炎沙門菌SDBL-1分離株的致病性和傳播途徑探究
發(fā)布時(shí)間：2018-11-12 16:33
【摘要】：沙門菌能夠?qū)е氯祟惖氖澄镏卸?有重要的公共衛(wèi)生學(xué)意義。本研究首先對(duì)部分地區(qū)不同品種雞群采集樣品分離沙門菌,從病原學(xué)和血清學(xué)兩方面對(duì)流行病學(xué)進(jìn)行了調(diào)查;其次選擇1株有代表性的腸炎沙門菌SDBL-1分離株對(duì)其生物學(xué)特性進(jìn)行探究,最后用腸炎沙門菌SDBL-1分離株對(duì)SPF雞進(jìn)行感染。初步探討了SDBL-1分離株對(duì)SPF雞的感染途徑及其致病性。為防控腸炎沙門菌的傳播奠定了科學(xué)基礎(chǔ)。不同沙門菌抗體診斷抗原及不同診斷方法對(duì)沙門菌抗體測(cè)定結(jié)果比較試驗(yàn)隨機(jī)選取210份樣品,其中血清樣品154份,卵黃樣品56份,用某公司4個(gè)批次的雞白痢、雞傷寒多價(jià)染色抗原以玻片凝集法測(cè)定雞白痢、雞傷寒抗體,比較同一廠家不同批次診斷抗原對(duì)其測(cè)定結(jié)果的差異;隨機(jī)選擇36份血清樣品,選擇一種商品化的雞白痢、雞傷寒多價(jià)染色抗原和實(shí)驗(yàn)室自制的腸炎沙門菌診斷抗原以玻片凝集法測(cè)定抗體;隨機(jī)選擇91份血清樣品,用實(shí)驗(yàn)室自制的腸炎沙門菌診斷抗原和國產(chǎn)、進(jìn)口的沙門菌抗體ELISA試劑盒測(cè)定抗體,比較結(jié)果差異性。試驗(yàn)結(jié)果表明,4個(gè)批次的雞白痢、雞傷寒多價(jià)染色抗原血清抗體測(cè)定吻合率在65.6%~89.6%之間,卵黃抗體測(cè)定的吻合率在98.2%~100%之間,商品化的診斷抗原與實(shí)驗(yàn)室自制診斷抗原的吻合率為61.1%,實(shí)驗(yàn)室自制抗原與國產(chǎn)及進(jìn)口的沙門菌抗體ELISA試劑盒吻合率均為69.2%。結(jié)果提示,不同診斷抗原及不同的診斷方法之間測(cè)定結(jié)果存在差異,各種方法之間不能完全代替。不同品種雞沙門菌的流行病學(xué)調(diào)查試驗(yàn)選取不同地區(qū)8個(gè)雞群,共計(jì)2971份血清樣品,用抗原抗體平板凝集試驗(yàn)測(cè)定沙門菌抗體。選取17個(gè)雞群,采集20胚齡的雞胚、臟器等樣品共計(jì)1658份,采用自行優(yōu)化的沙門菌分離方法,分離鑒定沙門菌。檢測(cè)結(jié)果表明,2971份血清樣品中雞白痢、雞傷寒陽性率為31.8%,不同雞群雞白痢、雞傷寒血清陽性率在6.2%~71.9%之間。1658份雞胚及臟器樣品中陽性樣品為80份,17個(gè)雞群中陽性雞群有8個(gè),不同雞群的陽性率在0%~31.5%之間。結(jié)果提示,所檢測(cè)雞群存在著沙門菌的感染。腸炎沙門菌SDBL-1分離株的致病性及傳播途徑探究試驗(yàn)選取腸炎沙門菌SDBL-1分離株探究其生物學(xué)特性,包括其生長(zhǎng)曲線、藥物敏感、毒力。以腸炎沙門菌SDBL-1分離株人工感染6周齡SPF雞(感染組),同時(shí)設(shè)立空氣傳播組和對(duì)照組,在感染后不同時(shí)間同時(shí)測(cè)定3組試驗(yàn)雞的體重、血液常規(guī)指標(biāo)、淋巴細(xì)胞亞群指標(biāo)及抗體變化和感染雞的排毒規(guī)律,并檢測(cè)環(huán)境(飼料、飲水、蚊蠅等)中的沙門菌。結(jié)果表明,腸炎沙門菌SDBL-1分離株的LD50為1.905×109CFU,其對(duì)青霉素g、苯唑西林、卡那霉素、紅霉素、麥迪霉素、呋喃妥因、萬古霉素、克拉霉素、克林霉素敏感;腸炎沙門菌SDBL-1分離株感染SPF雞對(duì)其體重沒有顯著影響(P0.05),但其淋巴細(xì)胞比率在2、4、6、8d時(shí)顯著下降,粒細(xì)胞比率、粒細(xì)胞數(shù)量在2、4、6、8d時(shí)以及中間細(xì)胞比率在4、6、8d時(shí)顯著上升;感染腸炎沙門菌SDBL-1分離株引起淋巴細(xì)胞亞群CD4+、CD8+比例分別在2d、4d先出現(xiàn)下降,在感染后的6d時(shí)達(dá)到最低,之后逐漸上升恢復(fù)平穩(wěn),空氣傳播組有相同趨勢(shì),但發(fā)生的時(shí)間推遲2d。而且,感染組及空氣傳播組在感染后的4d均檢測(cè)到抗體。結(jié)果提示,腸炎沙門菌SDBL-1分離株感染SPF雞后,會(huì)引起機(jī)體炎癥反應(yīng),造成免疫功能紊亂,引起器官不同程度損傷,沙門菌可通過空氣傳播,感染雞可通過糞便、口腔、泄殖腔間歇排毒。綜上所述,對(duì)于沙門菌的診斷,不同診斷抗原及不同診斷方法之間存在差異;不同地區(qū)雞群存在著沙門菌感染,主要以腸炎沙門菌為主;腸炎沙門菌SDBL-1會(huì)引起SPF雞感染,感染雞機(jī)體受到損傷,并且繼續(xù)向外界排出細(xì)菌,引起環(huán)境和雞群感染。
[Abstract]:Salmonella can cause food poisoning in humans and has important public health significance. In this study, the samples were collected from different breeds of chicken in some regions, and the epidemiology was investigated from the aspects of etiology and serology, and the biological characteristics of Salmonella enteritidis SDBL-1 were selected. The SPF chickens were infected with Salmonella enteritidis SDBL-1. The infection and pathogenicity of SDBL-1 isolates to SPF chickens were discussed. and lays a scientific foundation for preventing and controlling the spread of Salmonella enteritidis. A total of 210 samples were randomly selected from the test results of the different Salmonella antibody diagnostic antigen and the different diagnostic methods. The serum samples were 154 samples, the yolk samples were 56 samples, and four lots of chicken white diarrhea were used. the difference of the test results of the chicken white diarrhea and the chicken typhoid antibody is determined by a slide agglutination method by the chicken typhoid polyvalent staining antigen, 36 serum samples are randomly selected, a commercial chicken white diarrhea is selected, the antibody is detected by a slide agglutination method by a chicken typhoid multivalent stain antigen and a laboratory self-made Salmonella enteritidis diagnostic antigen; 91 serum samples are randomly selected, and an antibody is determined by using a laboratory self-made Salmonella enteritidis diagnostic antigen and a domestic and imported Salmonella antibody ELISA kit, and the difference of the results is compared. The results of the test showed that the matching rate of the antibody was 60.6% ~ 88.9%, the matching rate of the yolk antibody was 98.2% ~ 89. 6%, the coincidence rate of the commercial diagnostic antigen and the self-made diagnostic antigen of the laboratory was 61.1%. The coincidence rate between the self-made antigen of the laboratory and the domestic and imported Salmonella antibody ELISA kits was 69.2%. The results suggested that there was a difference in the results between the different diagnostic antigens and the different diagnostic methods, and the different methods could not be replaced completely. A total of 2971 serum samples from different regions were selected according to the epidemiological investigation of Salmonella in different regions, and the antibody of Salmonella was determined by the antigen-antibody plate agglutination test. A total of 1658 samples of 20-embryo-age chicken embryos and organs were collected, and the Salmonella were isolated and identified by self-optimized isolation method. The results showed that the positive rate of chicken white diarrhea and chicken typhoid in 2971 serum samples was 31. 8%, and the positive rate of chicken's white diarrhea and chicken typhoid in different chicken group was between 6.2% and 71.9%. The positive samples in 1658 chicken embryo and organ samples were 80, and there were 8 positive chickens in 17 chickens. The positive rate of different chicken groups was between 0% and 31.5%. The results suggested that there was a Salmonella infection in the detected chicken group. The pathogenicity and the transmission route of Salmonella enteritidis SDBL-1 isolate were investigated. The biological characteristics of Salmonella enteritidis SDBL-1 were studied, including its growth curve, drug sensitivity and virulence. A 6-week-old SPF chicken (infected group) was infected with Salmonella enteritidis SDBL-1, and the air-spreading group and the control group were set up, and the body weight, blood routine index, lymphocyte subgroup index and antibody change and the toxin-expelling rule of the infected chicken were measured at different time after infection. and the salmonella in the environment (feed, drinking water, mosquito and fly, etc.) is detected. The results showed that the LD50 of Salmonella enteritidis SDBL-1 isolate was 1. 905-109CFU, which was sensitive to penicillins g, penethamate, kanamycin, erythromycin, clindamycin, psoralen, vancomycin, clarithromycin and klincomycin. There was no significant effect on the body weight of the isolated strains of Salmonella enteritidis SDBL-1 (P0.05), but the lymphocyte ratio decreased significantly in 2, 4, 6, and 8 days, and the ratio of the granulocytes and the number of granulocytes in 2, 4, 6, and 8d and the intermediate cell ratio increased significantly at 4,6,8days. The proportion of CD4 + and CD8 + in the subpopulations of the lymphocyte subpopulations in the Salmonella enteritidis SDBL-1 isolates was decreased in the 2d and 4d, respectively. After the infection, the level of CD4 + and CD8 + reached the lowest after the infection, and then the recovery was stable. The air-spreading group had the same trend, but the time of the occurrence was delayed by 2d. In addition, the infection group and the air distribution group all detected the antibody after the infection. The results suggest that the Salmonella enteritidis SDBL-1 isolate can cause the inflammation of the body, cause the immune function disorder, cause the organ to different degree damage, the salmonella can spread through the air, the infected chicken can pass through the stool, the oral cavity and the cloaca chamber for intermittent detoxification. To sum up, there is a difference between different diagnostic antigens and different diagnostic methods for the diagnosis of Salmonella, and there are Salmonella infections in different regions of the chicken group, mainly in the form of Salmonella enteritidis; the Salmonella enteritidis SDBL-1 can cause the SPF chicken to be infected, and the infected chicken body is damaged, and continue to discharge the bacteria to the outside, causing the environment and the chicken population to be infected.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.31
本文編號(hào)：2327606
[Abstract]:Salmonella can cause food poisoning in humans and has important public health significance. In this study, the samples were collected from different breeds of chicken in some regions, and the epidemiology was investigated from the aspects of etiology and serology, and the biological characteristics of Salmonella enteritidis SDBL-1 were selected. The SPF chickens were infected with Salmonella enteritidis SDBL-1. The infection and pathogenicity of SDBL-1 isolates to SPF chickens were discussed. and lays a scientific foundation for preventing and controlling the spread of Salmonella enteritidis. A total of 210 samples were randomly selected from the test results of the different Salmonella antibody diagnostic antigen and the different diagnostic methods. The serum samples were 154 samples, the yolk samples were 56 samples, and four lots of chicken white diarrhea were used. the difference of the test results of the chicken white diarrhea and the chicken typhoid antibody is determined by a slide agglutination method by the chicken typhoid polyvalent staining antigen, 36 serum samples are randomly selected, a commercial chicken white diarrhea is selected, the antibody is detected by a slide agglutination method by a chicken typhoid multivalent stain antigen and a laboratory self-made Salmonella enteritidis diagnostic antigen; 91 serum samples are randomly selected, and an antibody is determined by using a laboratory self-made Salmonella enteritidis diagnostic antigen and a domestic and imported Salmonella antibody ELISA kit, and the difference of the results is compared. The results of the test showed that the matching rate of the antibody was 60.6% ~ 88.9%, the matching rate of the yolk antibody was 98.2% ~ 89. 6%, the coincidence rate of the commercial diagnostic antigen and the self-made diagnostic antigen of the laboratory was 61.1%. The coincidence rate between the self-made antigen of the laboratory and the domestic and imported Salmonella antibody ELISA kits was 69.2%. The results suggested that there was a difference in the results between the different diagnostic antigens and the different diagnostic methods, and the different methods could not be replaced completely. A total of 2971 serum samples from different regions were selected according to the epidemiological investigation of Salmonella in different regions, and the antibody of Salmonella was determined by the antigen-antibody plate agglutination test. A total of 1658 samples of 20-embryo-age chicken embryos and organs were collected, and the Salmonella were isolated and identified by self-optimized isolation method. The results showed that the positive rate of chicken white diarrhea and chicken typhoid in 2971 serum samples was 31. 8%, and the positive rate of chicken's white diarrhea and chicken typhoid in different chicken group was between 6.2% and 71.9%. The positive samples in 1658 chicken embryo and organ samples were 80, and there were 8 positive chickens in 17 chickens. The positive rate of different chicken groups was between 0% and 31.5%. The results suggested that there was a Salmonella infection in the detected chicken group. The pathogenicity and the transmission route of Salmonella enteritidis SDBL-1 isolate were investigated. The biological characteristics of Salmonella enteritidis SDBL-1 were studied, including its growth curve, drug sensitivity and virulence. A 6-week-old SPF chicken (infected group) was infected with Salmonella enteritidis SDBL-1, and the air-spreading group and the control group were set up, and the body weight, blood routine index, lymphocyte subgroup index and antibody change and the toxin-expelling rule of the infected chicken were measured at different time after infection. and the salmonella in the environment (feed, drinking water, mosquito and fly, etc.) is detected. The results showed that the LD50 of Salmonella enteritidis SDBL-1 isolate was 1. 905-109CFU, which was sensitive to penicillins g, penethamate, kanamycin, erythromycin, clindamycin, psoralen, vancomycin, clarithromycin and klincomycin. There was no significant effect on the body weight of the isolated strains of Salmonella enteritidis SDBL-1 (P0.05), but the lymphocyte ratio decreased significantly in 2, 4, 6, and 8 days, and the ratio of the granulocytes and the number of granulocytes in 2, 4, 6, and 8d and the intermediate cell ratio increased significantly at 4,6,8days. The proportion of CD4 + and CD8 + in the subpopulations of the lymphocyte subpopulations in the Salmonella enteritidis SDBL-1 isolates was decreased in the 2d and 4d, respectively. After the infection, the level of CD4 + and CD8 + reached the lowest after the infection, and then the recovery was stable. The air-spreading group had the same trend, but the time of the occurrence was delayed by 2d. In addition, the infection group and the air distribution group all detected the antibody after the infection. The results suggest that the Salmonella enteritidis SDBL-1 isolate can cause the inflammation of the body, cause the immune function disorder, cause the organ to different degree damage, the salmonella can spread through the air, the infected chicken can pass through the stool, the oral cavity and the cloaca chamber for intermittent detoxification. To sum up, there is a difference between different diagnostic antigens and different diagnostic methods for the diagnosis of Salmonella, and there are Salmonella infections in different regions of the chicken group, mainly in the form of Salmonella enteritidis; the Salmonella enteritidis SDBL-1 can cause the SPF chicken to be infected, and the infected chicken body is damaged, and continue to discharge the bacteria to the outside, causing the environment and the chicken population to be infected.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.31
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