四川部分地區(qū)山羊皮下膿腫的細(xì)菌學(xué)分析
發(fā)布時(shí)間：2018-11-12 15:36
【摘要】：山羊飼養(yǎng)歷史悠久,具有很高的經(jīng)濟(jì)價(jià)值。我國(guó)改革開(kāi)放30多年以來(lái),山羊畜牧產(chǎn)業(yè)發(fā)展迅速,占全國(guó)畜牧產(chǎn)業(yè)總產(chǎn)值的6%左右,四川作為山羊養(yǎng)殖大省,山羊存欄總量占據(jù)全國(guó)前五,顯得尤為重要。在畜牧生產(chǎn)中,山羊皮下膿腫是一種常見(jiàn)多發(fā)病,對(duì)養(yǎng)羊產(chǎn)業(yè)造成巨大的經(jīng)濟(jì)損失。近來(lái)對(duì)其的報(bào)道比較多,卻沒(méi)有很好的治療辦法,關(guān)鍵在于其病原菌來(lái)源的多樣性。針對(duì)此,本實(shí)驗(yàn)展開(kāi)對(duì)四川主要養(yǎng)羊地區(qū)的山羊皮下膿腫病原學(xué)調(diào)查,為判明四川省主要養(yǎng)羊區(qū)的山羊皮下或淋巴結(jié)膿腫的致病病原并為該病的預(yù)防治療提供依據(jù)。本研究于2015年4月至9月期間在四川宣漢、廣安、攀枝花、巴中、資陽(yáng)幾個(gè)重要養(yǎng)羊地區(qū)共采集47份山羊膿腫病料,分別在需氧和厭氧條件下培養(yǎng)分離純化出山羊皮下膿腫的致病菌。對(duì)分離純化的致病菌進(jìn)行菌落形態(tài)觀察和細(xì)菌生化特性分析,并結(jié)合16S rRNA基因序列測(cè)序分析進(jìn)行細(xì)菌鑒定。選擇致病菌測(cè)定其半數(shù)致死量(LDso)以判定其毒力強(qiáng)弱,并對(duì)鑒定的致病菌株進(jìn)行藥物敏感性試驗(yàn),篩選出致病菌株的敏感性藥物。1、對(duì)六個(gè)規(guī)模化養(yǎng)羊場(chǎng)共2440只存欄山羊進(jìn)行調(diào)查,有230只發(fā)病,該病在各羊場(chǎng)的發(fā)病率從8.36%-11.27%不等,平均發(fā)病率為9.43%。采集的47份病料中共分離得到118株細(xì)菌,其中革蘭氏陰性菌42株,占35.6%:包括大腸桿菌15株,占12.7%,彎曲假單胞菌27株,占22.9%：革蘭氏陽(yáng)性菌76株,占64.4%:包括偽結(jié)核棒狀桿菌22株,占18.6%,金黃色葡萄球菌13株,占11.0%,芽孢桿菌41株,占34.7%。2、對(duì)分離得到的致病菌偽結(jié)核棒狀桿菌和金黃色葡萄球菌進(jìn)行毒力檢測(cè),其中偽結(jié)核棒狀桿菌菌株對(duì)小鼠的致病力較強(qiáng),LD50為107.5cfu/ml,而金黃色葡萄球菌的LD50為109.7cfu/ml。3、對(duì)試驗(yàn)分離得到的22株偽結(jié)核棒狀桿菌和13株金黃色葡萄球菌采用美國(guó)臨床和實(shí)驗(yàn)室標(biāo)準(zhǔn)協(xié)會(huì)(CLSI)推薦的Kirby-Bauer紙片法分別進(jìn)行9種臨床常用抗菌藥物的耐藥性試驗(yàn),并選取標(biāo)準(zhǔn)菌株偽結(jié)核棒狀桿菌ATCC19410和金黃色葡萄球菌ATCC25923作為質(zhì)控菌株進(jìn)行試驗(yàn)結(jié)果可靠性驗(yàn)證。藥敏結(jié)果表明：試驗(yàn)菌株均表現(xiàn)出嚴(yán)重的耐藥性,但對(duì)藥物的敏感性不同。偽結(jié)核棒狀桿菌對(duì)利福平、復(fù)方新諾明、環(huán)丙沙星和鏈霉素具有一定的敏感性,對(duì)青霉素和慶大霉素耐藥。金黃色葡萄球菌對(duì)左氧氟沙星和利福平均具有較高的敏感性,對(duì)青霉素、氨芐西林、克林霉素和頭孢他啶耐藥。該研究發(fā)現(xiàn)偽結(jié)核棒狀桿菌和金黃色葡萄球菌為發(fā)病羊場(chǎng)的共同致病菌,并進(jìn)行了藥物篩選,為該地區(qū)山羊皮下膿腫病的防治提供了參考。
[Abstract]:Goat breeding has a long history and has high economic value. The goat animal husbandry industry has developed rapidly in China since the reform and opening up for more than 30 years, accounting for about 6% of the total output value of the national livestock industry. Sichuan, as a large province of goat farming, occupies the top five in the total amount of goat stock in the country, which is particularly important. In livestock production, goat subcutaneous abscess is a common disease, which causes huge economic loss to sheep industry. There are many reports about it recently, but there is no good treatment method. The key is the diversity of pathogen source. In order to identify the pathogeny of goat subcutaneous or lymph node abscess in the main goat raising area of Sichuan province and provide the basis for the prevention and treatment of the disease, this study investigated the etiology of goat subcutaneous abscess in the main goat raising area of Sichuan province. From April to September 2015, 47 goat abscesses were collected in Xuanhan, Guangan, Panzhihua, Bazhong and Ziyang, Sichuan Province. The pathogenic bacteria of goat subcutaneous abscess were isolated and purified under aerobic and anaerobic conditions. The colony morphology and biochemical characteristics of the isolated and purified pathogenic bacteria were observed and identified by 16s rRNA gene sequencing. In order to determine the virulence of pathogenic bacteria, (LDso) was selected to determine its virulence, and the susceptibility test of pathogenic strains was carried out to screen the sensitive drugs of pathogenic strains. A total of 2440 sheep were collected from six large-scale sheep farms. The incidence of the disease ranged from 8.36% to 11.27%, and the average incidence rate was 9.43%. A total of 118 strains of bacteria were isolated from 47 samples, 42 of which were Gram-negative bacteria, accounting for 35.6k.There were 15 strains of Escherichia coli, 12.7m, 27 strains of Pseudomonas flexor, 22.910 strains of Gram-positive bacteria, 76 strains of Gram-positive bacteria, 15 strains of Escherichia coli, 27 strains of Pseudomonas bending and 76 strains of Gram-positive bacteria. Accounting for 64.4%: including 22 strains of pseudotuberculosis Corynebacterium tuberculosis (18.6B), 13 strains of Staphylococcus aureus (11.0%), 41 strains of Bacillus, 34.70.2. The virulence of the isolated pathogenic bacteria, Corynebacterium tuberculosis and Staphylococcus aureus, was tested. The virulence of the strain was stronger in mice, LD50 was 107.5 cfurml. The LD50 of Staphylococcus aureus was 109.7 cfuml.3. A total of 22 strains of pseudotuberculosis and 13 strains of Staphylococcus aureus were tested for drug resistance of 9 common clinical antimicrobial agents by Kirby-Bauer disk method recommended by (CLSI) of American Association of Clinical and Laboratory Standards. The standard strains ATCC19410 and ATCC25923 were selected as the quality control strains to verify the reliability of the test results. The results of drug sensitivity showed that all of the tested strains showed severe drug resistance, but the sensitivity to drugs was different. Corynebacterium pseudotuberculosis is sensitive to rifampicin, compound sulfamethoxine, ciprofloxacin and streptomycin, and resistant to penicillin and gentamicin. Staphylococcus aureus showed high average sensitivity to levofloxacin and rifampicin, and was resistant to penicillin, ampicillin, clindamycin and ceftazidime. This study found that pseudotuberculosis corynebacterium and Staphylococcus aureus were the common pathogenic bacteria in sheep farm, and the drug screening was carried out, which provided a reference for the prevention and treatment of goat subcutaneous abscess disease in this area.
【學(xué)位授予單位】：四川農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S858.27
[Abstract]:Goat breeding has a long history and has high economic value. The goat animal husbandry industry has developed rapidly in China since the reform and opening up for more than 30 years, accounting for about 6% of the total output value of the national livestock industry. Sichuan, as a large province of goat farming, occupies the top five in the total amount of goat stock in the country, which is particularly important. In livestock production, goat subcutaneous abscess is a common disease, which causes huge economic loss to sheep industry. There are many reports about it recently, but there is no good treatment method. The key is the diversity of pathogen source. In order to identify the pathogeny of goat subcutaneous or lymph node abscess in the main goat raising area of Sichuan province and provide the basis for the prevention and treatment of the disease, this study investigated the etiology of goat subcutaneous abscess in the main goat raising area of Sichuan province. From April to September 2015, 47 goat abscesses were collected in Xuanhan, Guangan, Panzhihua, Bazhong and Ziyang, Sichuan Province. The pathogenic bacteria of goat subcutaneous abscess were isolated and purified under aerobic and anaerobic conditions. The colony morphology and biochemical characteristics of the isolated and purified pathogenic bacteria were observed and identified by 16s rRNA gene sequencing. In order to determine the virulence of pathogenic bacteria, (LDso) was selected to determine its virulence, and the susceptibility test of pathogenic strains was carried out to screen the sensitive drugs of pathogenic strains. A total of 2440 sheep were collected from six large-scale sheep farms. The incidence of the disease ranged from 8.36% to 11.27%, and the average incidence rate was 9.43%. A total of 118 strains of bacteria were isolated from 47 samples, 42 of which were Gram-negative bacteria, accounting for 35.6k.There were 15 strains of Escherichia coli, 12.7m, 27 strains of Pseudomonas flexor, 22.910 strains of Gram-positive bacteria, 76 strains of Gram-positive bacteria, 15 strains of Escherichia coli, 27 strains of Pseudomonas bending and 76 strains of Gram-positive bacteria. Accounting for 64.4%: including 22 strains of pseudotuberculosis Corynebacterium tuberculosis (18.6B), 13 strains of Staphylococcus aureus (11.0%), 41 strains of Bacillus, 34.70.2. The virulence of the isolated pathogenic bacteria, Corynebacterium tuberculosis and Staphylococcus aureus, was tested. The virulence of the strain was stronger in mice, LD50 was 107.5 cfurml. The LD50 of Staphylococcus aureus was 109.7 cfuml.3. A total of 22 strains of pseudotuberculosis and 13 strains of Staphylococcus aureus were tested for drug resistance of 9 common clinical antimicrobial agents by Kirby-Bauer disk method recommended by (CLSI) of American Association of Clinical and Laboratory Standards. The standard strains ATCC19410 and ATCC25923 were selected as the quality control strains to verify the reliability of the test results. The results of drug sensitivity showed that all of the tested strains showed severe drug resistance, but the sensitivity to drugs was different. Corynebacterium pseudotuberculosis is sensitive to rifampicin, compound sulfamethoxine, ciprofloxacin and streptomycin, and resistant to penicillin and gentamicin. Staphylococcus aureus showed high average sensitivity to levofloxacin and rifampicin, and was resistant to penicillin, ampicillin, clindamycin and ceftazidime. This study found that pseudotuberculosis corynebacterium and Staphylococcus aureus were the common pathogenic bacteria in sheep farm, and the drug screening was carried out, which provided a reference for the prevention and treatment of goat subcutaneous abscess disease in this area.
【學(xué)位授予單位】：四川農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S858.27
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