【摘要】：為分析并預(yù)測弓形蟲致密顆粒蛋白GRA12基因編碼蛋白的結(jié)構(gòu)、功能以及其作為疫苗候選抗原的可能性,本研究從Gen Bank數(shù)據(jù)庫獲取GRA12基因序列,通過在線蛋白分析專家系統(tǒng)(Expasy)并結(jié)合GENERUNR、DNAStar、DNAMAN等生物信息學(xué)分析軟件對GRA12序列的編碼區(qū)進行分析、對該基因編碼蛋白質(zhì)的理化性質(zhì)、信號肽、跨膜結(jié)構(gòu)域、二級結(jié)構(gòu)、親/疏水性、抗原表位等信息預(yù)測。結(jié)果表明:該基因全長1 311 bp,編碼436個氨基酸,蛋白性質(zhì)穩(wěn)定,理論分子質(zhì)量約為47.8 ku,無信號肽,有6個跨膜區(qū),14個絲氨酸磷酸化位點、7個蘇氨酸磷酸化位點、3個酪氨酸磷酸化位點、18個O-糖基化位點、3個乙�；鞍赘街稽c、1個酪氨酸硫酸化位點、1個潛在的N-糖基化位點、1個棕櫚�；稽c,15個潛在的抗原表位。研究結(jié)果分析說明GRA12有潛力成為弓形蟲疫苗候選抗原。
[Abstract]:In order to analyze and predict the structure and function of GRA12 gene encoding protein of Toxoplasma gondii compact granule protein and its possibility as vaccine candidate antigen, GRA12 gene sequence was obtained from Gen Bank database. The coding region of GRA12 sequence was analyzed by on-line protein analysis expert system (Expasy) and bioinformatics analysis software such as GENERUNR,DNAStar,DNAMAN. The physicochemical properties, signal peptide, transmembrane domain and secondary structure of the encoded protein were analyzed. Prediction of hydrophilic / hydrophobic and antigenic epitopes. The results showed that the total length of 1 311 bp, encodes 436 amino acids, and the protein is stable. The theoretical molecular weight is about 47.8 ku, signal free peptide, with 6 transmembrane regions, 14 serine phosphorylation sites and 7 threonine phosphorylation sites. There were 3 tyrosine phosphorylation sites, 18 O-glycosylation sites, 3 acetylated protein attachment sites, 1 tyrosine sulfation site, 1 potential N-glycosylation site, 1 palmitoacylation site and 15 potential antigen epitopes. The results show that GRA12 has the potential to be a vaccine candidate antigen for Toxoplasma gondii.
【作者單位】： 吉林農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;吉林省畜牧獸醫(yī)科學(xué)研究院;吉林大學(xué)畜牧獸醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金青年基金項目(31302076);國家自然科學(xué)基金(31472183) 吉林省留學(xué)人員科技創(chuàng)新創(chuàng)業(yè)項目(2012) 吉林省公益性科研單位基本科研專項社會公益研究(2060302)
【分類號】：S852.7
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本文編號：2316128