【摘要】：雞大腸桿菌病是由大腸桿菌引起的一種傳染病,具有發(fā)病率高、死亡率高的特點,嚴重影響?zhàn)B雞業(yè)發(fā)展。所以如何防控大腸桿菌病是亟待解決的問題。目前防治該病主要依靠大量使用抗生素,容易造成菌群失調(diào)、產(chǎn)生耐藥性和藥物殘留,極大影響雞產(chǎn)品質(zhì)量,}f食品安全造成極大隱患。本研究應用益生菌發(fā)酵中藥技術,對益生菌復方制劑進行篩選,對復方中藥進行配比和篩選,進行發(fā)酵液、中藥液體外抑菌試驗,并用對照組、感染組、中藥組、發(fā)酵液組、西藥組對雞大腸桿菌病進行治療和對比,用藥三天后,測定SIgA、病理組織切片、抗氧化指標,通過整理數(shù)據(jù)分析治療效果差異性。結果顯示保加利亞乳桿菌與植物乳桿菌(1:4)復方制劑OD值最大,明顯高于保加利亞乳桿菌、植物乳桿菌。選用18味中藥組成抗大腸桿菌的復方制劑:黃芪20g,黃連10g,烏梅10g,白頭翁30g,茯苓30g,黃芩20g,金銀花20g,大青葉10g,葛根30g,千里光20g,神曲15g,甘草20g,山楂15g,澤瀉10g,訶子10g,明礬10g,黃柏10g,麥芽25g,總計315g。發(fā)酵條件:溫度37℃,時間48h,轉(zhuǎn)速200rpm,接種量20mL,裝液量480mL。發(fā)酵液體外抑菌實驗的抑菌圈直徑為25mm,中藥體外抑菌實驗的抑菌圈直徑為20mm,發(fā)酵液體外抑菌效果要高于中藥抑菌效果。病理組織切片結果顯示發(fā)酵液組試驗雞的十二指腸、盲腸病理損傷情況明顯輕于中藥組和西藥組;SIgA檢測結果表明,發(fā)酵液組雞盲腸內(nèi)容物中SIgA水平高于西藥組,與中藥組差異不顯著,發(fā)酵液組雞十二指腸內(nèi)容物中SIgA水與其他各組差異不明顯。血清丙二醛含量檢測結果表明發(fā)酵液組丙二醛含量高于中藥組和西藥組;血清谷胱甘肽過氧化物酶含量結果表明,發(fā)酵液組該酶含量明顯高于中藥組和西藥組;肝臟超氧化物歧化酶濃度檢測結果表明發(fā)酵液組高于西藥組,而低于中藥組;血清超氧化物酶濃度檢測結果表明發(fā)酵液組高于西藥組和中藥組。結論:通過中藥組、西藥組、發(fā)酵液組體外抑菌試驗、臨床動物試驗、指標檢測試驗,確定發(fā)酵液對大腸桿菌的治療效果好于中藥治療效果。
[Abstract]:E. coli disease is an infectious disease caused by Escherichia coli, which has the characteristics of high incidence and high mortality, which seriously affects the development of chicken industry. Therefore, how to prevent and control Escherichia coli disease is an urgent problem to be solved. At present, the prevention and treatment of the disease mainly rely on the use of a large number of antibiotics, which is easy to cause bacteriological disorders, drug resistance and drug residues, greatly affect the quality of chicken products,} f food safety caused great hidden trouble. In this study, probiotic fermentation Chinese medicine technology was used to screen probiotic compound formulations, mixture and screening of compound Chinese medicine, fermentation broth, liquid external bacteriostatic test of traditional Chinese medicine, and control group, infection group, traditional Chinese medicine group, fermentation broth group, and the control group, infection group, Chinese medicine group, fermentation broth group. The treatment and comparison of chicken colibacillosis were carried out in the western medicine group. After three days of treatment, the pathological sections of SIgA, and the antioxidant index were determined, and the difference of treatment effect was analyzed by sorting out the data. The results showed that the OD value of the compound preparation of Lactobacillus bulgaricus and Lactobacillus plantarum (1:4) was higher than that of Lactobacillus bulgaricus and Lactobacillus plantarum. 18 Chinese herbs were used to make up the compound preparation of anti-Escherichia coli: astragalus 20g, Coptis chinensis 10g, Wupai 10g, wurin 30g, Scutellaria baicalensis 20g, honeysuckle 20g, big blue leaf 10g, kudzu root 30g, Qianliguang 20g, Shenqu 15g, licorice 20g, Hawthorn 15g. Alisma 10g, Chebula chebula 10g, alum 10g, juniper 10g, malt 25g, total 315g. The fermentation conditions were as follows: temperature 37 鈩，

本文編號：2308164