豬Pit-1基因多態(tài)性及其與生長(zhǎng)性狀的關(guān)聯(lián)分析
發(fā)布時(shí)間:2018-10-25 14:15
【摘要】:利用現(xiàn)代分子標(biāo)記技術(shù)尋找影響豬生長(zhǎng)性狀的標(biāo)記基因座,為應(yīng)用標(biāo)記輔助選擇培育高生產(chǎn)性能豬的品種或品系提供幫助。本試驗(yàn)采用PCR-RFLP(RsaⅠ限制性內(nèi)切酶)分子標(biāo)記技術(shù),分析了杜洛克、長(zhǎng)白、大白及長(zhǎng)大二元雜交4個(gè)品種共381頭豬Pit-1基因exon 5至intron 5內(nèi)多態(tài)性,并采用最小二乘法分析了Pit-1基因多態(tài)性對(duì)生長(zhǎng)性狀影響的遺傳效應(yīng)。經(jīng)檢測(cè),4個(gè)品種Pit-1基因RsaⅠ位點(diǎn)均存在多態(tài)性。等位基因A的基因頻率分別為0.831 7、0.830 2、0.676 7、0.752 3,A為優(yōu)勢(shì)基因。被測(cè)豬群Pit-1基因的基因頻率在被測(cè)豬群中均處于Hardy-Weinberg平衡狀態(tài)(p0.05)。最小二乘分析表明:Pit-1基因3種基因型的豬在出生重、斷奶重及70日齡重性狀上均呈現(xiàn)BBABAA趨勢(shì)(其中,長(zhǎng)白豬只存在AB,AA 2種基因型),B為優(yōu)勢(shì)基因。Pit-1基因exon 5至intron 5 RsaⅠ位點(diǎn)多態(tài)性對(duì)測(cè)試豬群生長(zhǎng)性狀有一定影響,但是否是影響生長(zhǎng)性狀的一個(gè)候選基因之一或是與影響豬生長(zhǎng)性狀的QTL連鎖的基因,還需進(jìn)一步研究。
[Abstract]:Using the modern molecular marker technology to find the marker loci which affect the growth traits of pigs, it is helpful for the selection and breeding of high performance pig breeds or strains with marker-assisted selection. The polymorphism of Pit-1 gene exon 5 to intron 5 in 381 pigs was analyzed by PCR-RFLP (Rsa 鈪,
本文編號(hào):2293936
[Abstract]:Using the modern molecular marker technology to find the marker loci which affect the growth traits of pigs, it is helpful for the selection and breeding of high performance pig breeds or strains with marker-assisted selection. The polymorphism of Pit-1 gene exon 5 to intron 5 in 381 pigs was analyzed by PCR-RFLP (Rsa 鈪,
本文編號(hào):2293936
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