【摘要】：布氏桿菌病(Brucellosis),簡稱布病,是一種由布氏桿菌(Brucella)引起的人獸共患傳染病。近年來,甘肅省的綿羊布病疫情呈現(xiàn)上升趨勢,不僅制約綿羊養(yǎng)殖業(yè)的持續(xù)發(fā)展,而且影響人民群眾的身體健康。本論文對2014年甘肅省三個地區(qū)綿羊布病疫情進行調(diào)查。1.在無“金標準”情況下,為評價RBT和I-ELISA對綿羊布病的診斷能力,本研究用RBT和I-ELISA對采自某地的176份未免疫綿羊血清同時進行檢測,通過建立貝葉斯模型,計算兩種方法的靈敏度和特異度。結果顯示,RBT和I-ELISA的靈敏度分別為76.5%、89.6%,特異度分別為95.1%、93.4%,I-ELISA的診斷能力更好。2.在靖遠縣、崆峒區(qū)和永昌縣的54個養(yǎng)殖場采集血清1536份,經(jīng)I-ELISA檢測,個體總陽性率為3.58%(55/1536),群體總陽性率為25.93%(14/54)。靖遠縣、崆峒區(qū)和永昌縣的個體陽性率分別為3.35%(18/538)、6.02%(30/498)和1.40%(7/500);群體陽性率分別為22.22%(2/9)、33.33%(11/33)和8.30%(1/12)。3.采集流產(chǎn)胎兒病料,用布氏桿菌培養(yǎng)基分別在靖遠縣、崆峒區(qū)和永昌縣分離得到1株布氏桿菌,依次命名為GS0250、GS2620和GS2638。用傳統(tǒng)方法、AMOS-PCR和MLVA-16對3株布氏桿菌進行種型鑒定。傳統(tǒng)方法將3株菌均鑒定為羊種3型布氏桿菌、AMOS-PCR擴增均得到731bp的特異性片段,將其鑒定為羊種布氏桿菌。3株菌的MLVA型為1-5-3-13-2-2-3-2-4-23-8-3-4-3-8-5,編號0063,與其它93個國內(nèi)羊種布氏桿菌分離株的型不同,親緣關系較遠,具有獨特性。3株菌聚在一起,提示同一地理來源的可能。
[Abstract]:Brucellosis (Brucellosis),) is a zoonotic infectious disease caused by brucellosis (Brucella). In recent years, the epidemic situation of sheep cloth disease in Gansu Province is on the rise, which not only restricts the sustainable development of sheep breeding industry, but also affects the health of the people. In this paper, we investigated the epidemic situation of sheep cloth disease in three areas of Gansu province in 2014. 1. In order to evaluate the diagnostic ability of RBT and I-ELISA in sheep brucellosis without gold standard, RBT and I-ELISA were used to simultaneously detect 176 unimmunized sheep sera collected from a certain place, and a Bayesian model was established. The sensitivity and specificity of the two methods were calculated. The results showed that the sensitivity of RBT and I-ELISA were 76. 5% and 89. 6, and the specificity was 95. 1% and 93. 4% Elisa had better diagnostic ability. 2. 1536 serum samples were collected from 54 farms in Jingyuan County, Kongtong District and Yongchang County. The total positive rate of individuals was 3.58% (55 / 1536) and the total positive rate of population was 25.93% (14 / 54) by I-ELISA. The positive rates of individuals in Jingyuan County, Kongtong District and Yongchang County were 3.35% (18 / 538), 6.02% (30 / 498) and 1.40% (7 / 500) respectively, and the positive rates of population were 22.22% (2 / 9), 33.33% (11 / 33) and 8.30% (1 / 12) respectively. A strain of brucellosis was isolated from Jingyuan County, Kongtong District and Yongchang County in Jingyuan County, Kongtong District and Yongchang County by using brucellosis medium, which was named GS0250,GS2620 and GS2638. in turn. Three strains of Brucella were identified by AMOS-PCR and MLVA-16 by traditional methods. By traditional method, three strains of Brucella sp. 3 were identified as Brucella ovis, and the specific fragments of 731bp were obtained by AMOS-PCR amplification. The MLVA type of the three strains was 1-5-3-13-2-2-2-2-2-4-4-23-8-3-3-8-5, no. 0063, which was different from that of other 93 domestic strains of Brucella. Three strains of bacteria gathered together, indicating the possibility of the same geographical origin.
【學位授予單位】：甘肅農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.26

【參考文獻】

相關期刊論文 前2條

1 顧海雁,陳啟光;無金標準情況下診斷試驗的評價方法[J];中國衛(wèi)生統(tǒng)計;1999年04期

2 齊景文;布魯氏菌病概述[J];中國獸醫(yī)雜志;2004年09期

，

本文編號：2280354