【摘要】：新疆伊犁某地區(qū)一些馬群中不斷出現(xiàn)以全身多發(fā)性膿腫為特征的患病馬匹,為了查明其是否為馬腺疫,以及由何種馬鏈球菌感染所致,無菌采集典型患病馬匹膿汁樣品,常規(guī)THB增菌,綿羊鮮血平板劃線分離單菌落后,用透射電鏡對(duì)分離菌的微觀形態(tài)進(jìn)行觀察,PCR對(duì)其fneB基因進(jìn)行克隆與序列分析,最后用生化試驗(yàn)進(jìn)行驗(yàn)證。結(jié)果表明,從12匹患病馬的膿腫組織中,共采集了25份樣品,從中分離鑒定出來源于不同患病馬的2株馬鏈球菌馬亞種菌株,這2株菌的形態(tài)均符合鏈球菌的特點(diǎn);基因克隆測(cè)序結(jié)果表明,其中1株與英國源的獸疫亞種H70和馬亞種4047核苷酸同源性較高,均達(dá)97%~98%,另1株與英國源的馬亞種4047和瑞典源某馬亞種核苷酸同源性較高,均達(dá)98%~99%。這2株菌之間核苷酸序列同源性達(dá)98.3%,氨基酸同源性則達(dá)99.3%。馬鏈球菌馬亞種是引起伊犁某地區(qū)這些患馬全身多發(fā)性膿腫的主要致病菌,它們可能來源于之前從歐洲和美洲引進(jìn)的馬匹,提示在進(jìn)行疫病防控工作時(shí),要特別注意海關(guān)檢疫,積極防止疫病的傳入。
[Abstract]:In a certain area of Yili, Xinjiang, some horses have been found to be sick with multiple abscesses. In order to find out whether the horses are infected with adenosis and by what kind of Streptococcus equine infection, aseptic samples of pus from a typical sick horse are collected. The micromorphology of the isolated bacteria was observed by transmission electron microscope (TEM), the fneB gene was cloned and sequenced by PCR, and the results were verified by biochemical test. The results showed that 25 samples were collected from the abscess tissues of 12 diseased horses. Two strains of Streptococcus equine Maya strains from different diseased horses were isolated and identified, and the morphology of the two strains were all in accordance with the characteristics of streptococcus. The results of gene cloning and sequencing showed that one strain had a high nucleotide homology with 4047 nucleotides of H70 and Maya, and the other had high nucleotide homology with Maya 4047 and Maya from Sweden. The nucleotide sequence homology and amino acid homology between the two strains were 98.3 and 99.3 respectively. The Maya strain of Streptococcus equi is the main pathogen causing multiple abscesses of horses in a certain area of Yili. They may have originated from horses imported from Europe and America, suggesting that special attention should be paid to customs quarantine in the prevention and control of epidemic diseases. Actively prevent the introduction of disease.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)學(xué)院;新疆伊犁種馬場(chǎng);
【基金】：國家科技支撐計(jì)劃項(xiàng)目(2012BAD46B02)
【分類號(hào)】：S852.611

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