【摘要】：妊娠相關(guān)糖蛋白(pregnancy-associated glycoprotein,PAG)是一類動物妊娠后胎盤產(chǎn)生的特異性蛋白質(zhì),是判斷妊娠的重要信號物質(zhì)。為了獲得綿羊妊娠相關(guān)糖蛋白PAG3的制備方法,以期通過檢測綿羊體內(nèi)的妊娠相關(guān)糖蛋白的含量來進行早期妊娠診斷。本試驗通過原核表達生物工程合成的方法,將綿羊妊娠相關(guān)糖蛋白編碼基因序列插入到表達載體pET20b中,利用原核表達轉(zhuǎn)化大腸桿菌獲得了融合His標簽的目的蛋白PAG3。經(jīng)過超聲波裂解、His標簽親和層析的方法純化獲得了妊娠相關(guān)糖蛋白PAG3。
[Abstract]:Pregnancy-associated glycoprotein (pregnancy-associated glycoprotein,PAG) is a kind of specific protein produced by placenta after pregnancy. In order to obtain the preparation method of pregnancy associated glycoprotein (PAG3) in sheep, we hope to detect the content of pregnancy-associated glycoprotein in sheep for early pregnancy diagnosis. In this experiment, the encoding gene sequence of sheep pregnancy related glycoprotein was inserted into the expression vector pET20b by prokaryotic expression of bioengineering synthesis. The target protein PAG3. of fusion His tag was obtained by prokaryotic expression transformation into E. coli. Pregnancy associated glycoprotein (PAG3.) was purified by ultrasonic lysis and affinity chromatography with His label.
【作者單位】： 河北農(nóng)業(yè)大學(xué)動物科技學(xué)院;
【基金】：國家絨毛羊產(chǎn)業(yè)技術(shù)體系資助項目(CARS-40)
【分類號】：S826
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本文編號：2269745