【摘要】：豬偽狂犬病是由豬偽狂犬病病毒引起的豬烈性傳染病,每年造成了數(shù)十億元的直接經(jīng)濟(jì)損失。本文從豬偽狂犬病疫苗免疫保護(hù)率的血清學(xué)調(diào)查、疫苗免疫程序制定及病毒凈化,探討臨床豬偽狂犬病防控技術(shù),為其提供理論依據(jù)和臨床實踐經(jīng)驗。對介休地區(qū)部分規(guī)模養(yǎng)豬場和散養(yǎng)戶血清樣本血清學(xué)調(diào)查,規(guī)模豬場疫苗抗體平均陽性率達(dá)91%,野毒抗體平均陽性率為2.6%；散養(yǎng)戶疫苗抗體保護(hù)率較低,平均陽性率僅為72.7%,野毒抗體平均陽性率為23.3%。規(guī)模豬場的豬偽狂犬野毒感染均不高但散養(yǎng)戶野毒感染偏高。為制定商品豬豬偽狂犬病疫苗免疫程序,試驗仔豬首免后14、21、28天,二免35天和三免35天測定血清中豬偽狂犬病病毒特異性抗體水平。對有豬偽狂犬野毒感染的豬場實施滴鼻免疫,效果理想；對保育期有感染的豬場在35日齡實施肌肉注射免疫,抗體水平較理想；對育肥期有感染的豬場,在70日齡實施肌肉注射免疫,效果較好。豬場應(yīng)根據(jù)自己生產(chǎn)中的具體問題,合理制定免疫程序。制定“免疫—檢測—淘汰—監(jiān)測”循環(huán)體系,多次循環(huán)后,祖代豬偽狂犬野毒血清抗體檢測均為陰性,豬偽狂犬病疫苗首免后,血清疫苗抗體顯示為陽性；父母代豬血清抗體在初次免疫4周后均顯示為陽性,成功完成自繁自養(yǎng)的豬場豬偽狂犬病凈化。
[Abstract]:Porcine pseudorabies virus (PRV) is an infectious disease of swine caused by pseudorabies virus (PRV), which causes billions of yuan of direct economic losses every year. Based on the serological investigation of immune protection rate of porcine pseudorabies vaccine, the formulation of vaccine immunization procedure and virus purification, the prevention and control techniques of pseudorabies in pigs were discussed in this paper, which provided theoretical basis and clinical experience for the prevention and control of pseudorabies. The serological investigation of serum samples from some scale pig farms and scattered households in Jiexiu area showed that the average positive rate of vaccine antibody and wild virus antibody were 91% and 2.6% respectively, and the protective rate of vaccine antibody was lower in the bulk family, the average positive rate of vaccine antibody was 91%, and the average positive rate of wild virus antibody was 2.6% in Jiexiu area. The average positive rate was only 72.7 and the average positive rate of wild antibody was 23.3. The infection rate of pseudorabies wild virus in pig farm was not high, but the infection rate of wild virus was higher in bulk farm. In order to establish the immunization procedure of porcine pseudorabies vaccine, the specific antibody levels of porcine pseudorabies virus in serum were measured at 14 ~ 21g / 28 days, 35 days after first immunization and 35 days after immunization for three days after the first immunization of piglets. The effect of nasal immunization on pig farms infected with pseudorabies virus is ideal. The antibody level of infected pig farms in the care period is better than that of the pigs infected in fattening stage. At 70 days of age, the effect of intramuscular immunization is better. Pig farms should be based on their own production of specific problems, reasonable formulation of immunization procedures. The circulating system of "immuno-examination-elimination monitoring" was established. After repeated cycles, the serum antibodies of pig pseudorabies virus of ancestral generation were all negative, and the antibody of serum vaccine was positive after the first immunization of pig pseudorabies vaccine. The serum antibodies of parents on behalf of pigs were all positive after the first immunization for 4 weeks, and the purification of pseudorabies was successfully completed.
【學(xué)位授予單位】：山西農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28

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