豬胸膜肺炎放線桿菌小鼠感染模型的建立及在體內(nèi)移行分布的研究
發(fā)布時間：2018-09-08 15:11
【摘要】：豬傳染性胸膜肺炎(Porcine Contagious Pleuropneumonia, PCP)是胸膜肺炎放線桿菌（Actinobacillus pleuropneumoniae,APP）引起的一種高度傳染性、致死性豬呼吸道傳染病。近年來，規(guī)�；i場的保育豬多發(fā)，APP有15個血清型，我國主要有血清1型、血清3型、血清5型和7型，本病的致病機制尚不明確，為揭示病原感染后各組織臟器的病理變化及體內(nèi)的移行分布規(guī)律，本實驗擬建立豬傳染性胸膜肺炎放線桿菌病的動物模型。 實驗采用豬傳染性胸膜肺炎放線桿菌7型菌株，通過人工途徑感染實驗動物，探索其對實驗動物的易感性和最佳感染途徑，并利用免疫組化的方法檢測動物感染后，其在模型動物體內(nèi)的移行分布規(guī)律。 實驗結(jié)果表明APP-7型菌株對小鼠具有較強的致病性，對小鼠的半數(shù)致死量為6.7×107CFU，篩選出的最佳感染途徑為腹腔注射。通過對小鼠感染后的臨床癥狀、組織器官的病理變化和死亡率等情況觀察，發(fā)現(xiàn)與豬臨床感染本病的表現(xiàn)具有高度的相似性，確定小鼠適合作為豬傳染性胸膜肺炎放線桿菌感染試驗的理想動物模型。免疫組化結(jié)果顯示，細(xì)菌感染小鼠2小時后，，小鼠肺臟中能檢測到細(xì)菌存在，感染4小時后，脾臟能檢測到細(xì)菌的存在，隨著感染時間的延長在小鼠肝臟、腎臟、心臟等主要組織器官中相繼監(jiān)測到細(xì)菌的分布，從感染后小鼠體內(nèi)各臟器中再分離的細(xì)菌與攻毒菌株一致。 本研究通過感染小鼠試驗，成功建立了APP-7菌株的小鼠動物模型，探索在細(xì)菌感染小鼠的不同時間后，體內(nèi)各主要組織器官細(xì)菌的分布規(guī)律，揭示了小鼠組織器官內(nèi)細(xì)菌的分布情況與造成動物機體組織損傷之間的關(guān)系，為進一步研究豬傳染性胸膜肺炎放線桿菌的致病機理，疫苗的研發(fā)以及本病的科學(xué)防治奠定堅實的理論基礎(chǔ)。
[Abstract]:Porcine infectious pleuropneumonia (Porcine Contagious Pleuropneumonia, PCP) is a highly infectious and fatal respiratory infection caused by Actinobacillus pleuropneumoniae (Actinobacillus pleuropneumoniae,APP). In recent years, there are 15 serotypes of app in large scale pig farms. In China, serotype 1, serotype 3, serotype 5 and serotype 7 are the main serotypes. The pathogenesis of the disease is not clear. In order to reveal the pathological changes of tissues and organs and the translocation and distribution of organs after pathogen infection, an animal model of actinomycosis of porcine infectious pleuropneumoniae was established in this experiment. In the experiment, Actinobacillus pleuropneumoniae type 7 was used to infect experimental animals by artificial way, to explore the susceptibility and the best way of infection to the experimental animals, and to detect the infection of animals by immunohistochemical method. Its migration and distribution in model animals. The results showed that the strain of APP-7 had strong pathogenicity to mice, and the median lethal dose to mice was 6.7 脳 107 CFU. The best route of infection was intraperitoneal injection. By observing the clinical symptoms, pathological changes of tissues and organs and mortality after infection in mice, it was found that there was a high similarity between the clinical manifestations of the disease and that of pigs. To determine that mice are suitable as an ideal animal model for actinobacillus pleuropneumoniae infection test. The results of immunohistochemistry showed that the presence of bacteria could be detected in the lungs of mice 2 hours after bacterial infection, and 4 hours after infection, the presence of bacteria could be detected in the spleen of mice with the prolongation of infection time in the liver and kidney of mice. The distribution of bacteria was detected in the heart and other major tissues and organs, and the bacteria isolated from the organs of the infected mice were consistent with the strains that attacked the virus. In this study, the animal model of APP-7 strain was successfully established by the experiment of infected mice, and the distribution of bacteria in main tissues and organs of mice was explored after different time of bacterial infection. In order to further study the pathogenic mechanism of Actinobacillus pleuropneumoniae in swine, the relationship between the distribution of bacteria in tissues and organs of mice and the injury of animal tissues was revealed. The research and development of vaccine and the scientific prevention and cure of this disease lay a solid theoretical foundation.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28
本文編號：2230884
[Abstract]:Porcine infectious pleuropneumonia (Porcine Contagious Pleuropneumonia, PCP) is a highly infectious and fatal respiratory infection caused by Actinobacillus pleuropneumoniae (Actinobacillus pleuropneumoniae,APP). In recent years, there are 15 serotypes of app in large scale pig farms. In China, serotype 1, serotype 3, serotype 5 and serotype 7 are the main serotypes. The pathogenesis of the disease is not clear. In order to reveal the pathological changes of tissues and organs and the translocation and distribution of organs after pathogen infection, an animal model of actinomycosis of porcine infectious pleuropneumoniae was established in this experiment. In the experiment, Actinobacillus pleuropneumoniae type 7 was used to infect experimental animals by artificial way, to explore the susceptibility and the best way of infection to the experimental animals, and to detect the infection of animals by immunohistochemical method. Its migration and distribution in model animals. The results showed that the strain of APP-7 had strong pathogenicity to mice, and the median lethal dose to mice was 6.7 脳 107 CFU. The best route of infection was intraperitoneal injection. By observing the clinical symptoms, pathological changes of tissues and organs and mortality after infection in mice, it was found that there was a high similarity between the clinical manifestations of the disease and that of pigs. To determine that mice are suitable as an ideal animal model for actinobacillus pleuropneumoniae infection test. The results of immunohistochemistry showed that the presence of bacteria could be detected in the lungs of mice 2 hours after bacterial infection, and 4 hours after infection, the presence of bacteria could be detected in the spleen of mice with the prolongation of infection time in the liver and kidney of mice. The distribution of bacteria was detected in the heart and other major tissues and organs, and the bacteria isolated from the organs of the infected mice were consistent with the strains that attacked the virus. In this study, the animal model of APP-7 strain was successfully established by the experiment of infected mice, and the distribution of bacteria in main tissues and organs of mice was explored after different time of bacterial infection. In order to further study the pathogenic mechanism of Actinobacillus pleuropneumoniae in swine, the relationship between the distribution of bacteria in tissues and organs of mice and the injury of animal tissues was revealed. The research and development of vaccine and the scientific prevention and cure of this disease lay a solid theoretical foundation.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28
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