延邊黃牛ANGPTL4基因組織表達(dá)規(guī)律與肌內(nèi)脂肪沉積相關(guān)性研究
發(fā)布時(shí)間：2018-09-01 14:25
【摘要】：為分析ANGPTL4基因在延邊黃牛各組織中的表達(dá)差異,進(jìn)一步探討該基因在肌內(nèi)脂肪沉積中的作用,本實(shí)驗(yàn)利用q RT-PCR法比較分析了ANGPTL4基因在延邊黃牛7個(gè)組織(心、肝、脾、肺、腎、背最長肌和后腿肌)中的表達(dá)水平及其與肌內(nèi)脂肪沉積的相關(guān)性。結(jié)果表明:ANGPTL4基因在7個(gè)組織中表達(dá)水平從高到低依次為肝、肺、腎、心、脾、背最長肌和后腿肌,肝中表達(dá)量極顯著高于心、脾、肺、腎、后腿肌和背最長肌組織(P0.01),肺中表達(dá)量顯著高于心、脾、腎、后腿肌和背最長肌組織(P0.05);背最長肌中ANGPTL4基因的表達(dá)量與背最長肌的IMF含量呈顯著正相關(guān)(P0.05),相關(guān)系數(shù)為0.924;ANGPTL4基因在后腿肌的表達(dá)量與后腿肌的IMF含量呈顯著正相關(guān)(P0.05),相關(guān)系數(shù)為0.998。結(jié)果顯示,ANGPTL4基因影響延邊黃牛肌內(nèi)脂肪沉積。
[Abstract]:In order to analyze the difference of expression of ANGPTL4 gene in the tissues of Yanbian cattle, and to explore the role of ANGPTL4 gene in intramuscular fat deposition, ANGPTL4 gene was compared in seven tissues (heart, liver, spleen, lung, kidney) of Yanbian cattle by using Q RT-PCR method. The expression level of longissimus dorsi and hind leg muscle and its correlation with intramuscular fat deposition. The results showed that the expression levels of the gene in liver, lung, kidney, heart, spleen, longissimus dorsi and hind leg were significantly higher than those in heart, spleen, lung and kidney. The expression in the lung was significantly higher than that in the heart, spleen and kidney (P0.01) in the hind leg muscle and the longissimus dorsi muscle (P0.01). The expression of ANGPTL4 gene in the longissimus dorsi muscle was positively correlated with the IMF content of the longissimus dorsi muscle (P0.05), the correlation coefficient was 0.924 ANGPTL4 gene expression in the leg muscle and the IMF content in the hind leg muscle (P0.05), the correlation coefficient was 0.998. The results showed that ANGPTL 4 gene affected intramuscular fat deposition in Yanbian cattle.
【作者單位】： 延邊大學(xué)農(nóng)學(xué)院;
【基金】：吉林省科技發(fā)展計(jì)劃重點(diǎn)科技攻關(guān)項(xiàng)目(20160204017NY) 吉林省現(xiàn)代肉牛產(chǎn)業(yè)技術(shù)體系建設(shè)研究課題(201635) 吉林省教育廳“十三五”科學(xué)技術(shù)項(xiàng)目
【分類號】：Q78;S823.81
本文編號：2217478
[Abstract]:In order to analyze the difference of expression of ANGPTL4 gene in the tissues of Yanbian cattle, and to explore the role of ANGPTL4 gene in intramuscular fat deposition, ANGPTL4 gene was compared in seven tissues (heart, liver, spleen, lung, kidney) of Yanbian cattle by using Q RT-PCR method. The expression level of longissimus dorsi and hind leg muscle and its correlation with intramuscular fat deposition. The results showed that the expression levels of the gene in liver, lung, kidney, heart, spleen, longissimus dorsi and hind leg were significantly higher than those in heart, spleen, lung and kidney. The expression in the lung was significantly higher than that in the heart, spleen and kidney (P0.01) in the hind leg muscle and the longissimus dorsi muscle (P0.01). The expression of ANGPTL4 gene in the longissimus dorsi muscle was positively correlated with the IMF content of the longissimus dorsi muscle (P0.05), the correlation coefficient was 0.924 ANGPTL4 gene expression in the leg muscle and the IMF content in the hind leg muscle (P0.05), the correlation coefficient was 0.998. The results showed that ANGPTL 4 gene affected intramuscular fat deposition in Yanbian cattle.
【作者單位】： 延邊大學(xué)農(nóng)學(xué)院;
【基金】：吉林省科技發(fā)展計(jì)劃重點(diǎn)科技攻關(guān)項(xiàng)目(20160204017NY) 吉林省現(xiàn)代肉牛產(chǎn)業(yè)技術(shù)體系建設(shè)研究課題(201635) 吉林省教育廳“十三五”科學(xué)技術(shù)項(xiàng)目
【分類號】：Q78;S823.81
【相似文獻(xiàn)】
相關(guān)期刊論文 前4條
1 李博;李偉;王恬;;肌內(nèi)脂肪細(xì)胞生長及調(diào)控因子的研究進(jìn)展[J];食品工業(yè)科技;2013年22期
2 江新業(yè),宋煥祿;部分家禽肉肌內(nèi)脂肪及脂肪酸含量的測定與分析[J];無錫輕工大學(xué)學(xué)報(bào);2004年05期
3 張立霞;高旭;宋建臣;孫平杰;夏曉輝;許應(yīng)天;;延邊黃牛白細(xì)胞介素18基因的克隆及序列分析[J];中國畜牧獸醫(yī);2012年05期
4 吳瓊;邢秀梅;榮敏;楊福合;;中國環(huán)頸雉肌內(nèi)脂肪相關(guān)候選基因的克隆與序列分析[J];特產(chǎn)研究;2009年03期
，本文編號：2217478
本文鏈接：http://sikaile.net/yixuelunwen/dongwuyixue/2217478.html
最近更新
教材專著
